×
  • Share
  • Email
  • Embed
  • Like
  • Private Content
 

Genotyping by sequencing on barley with Ion PGM™ Sequencer

by on Jun 05, 2012

  • 1,986 views

Drs. Nils Stein (IPK, Gatersleben, Germany) and Jesse Poland (USDA-ARS2, Manhattan, KS, USA) partnered with Life Technologies to develop a protocol for plant genotyping by sequencing (GBS) in barley ...

Drs. Nils Stein (IPK, Gatersleben, Germany) and Jesse Poland (USDA-ARS2, Manhattan, KS, USA) partnered with Life Technologies to develop a protocol for plant genotyping by sequencing (GBS) in barley using two restriction enzymes. From their first pilot, they concluded that Ion PGM™ Sequencer has a great potential for large GBS studies due to the high SNP calling accuracy, attractive cost per sample, and unmatched speed in the sequencing workflow.

Whether used for the discovery and identification of SNPs or as a screen for panels of thousands of known markers, genotyping by sequencing (GBS) using next-generation sequencing technologies is becoming increasingly important as a cost-effective and unique tool for association studies and genomics-assisted breeding in a range of plant species—including those with complex genomes that lack a reference sequence. The Ion PGM™ Sequencer is the perfect tool for GBS due to its scalability, simplicity, and speed.

Statistics

Views

Total Views
1,986
Views on SlideShare
1,986
Embed Views
0

Actions

Likes
1
Downloads
36
Comments
0

0 Embeds 0

No embeds

Accessibility

Categories

Upload Details

Uploaded via SlideShare as Adobe PDF

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
Post Comment
Edit your comment

Genotyping by sequencing on barley with Ion PGM™ Sequencer Genotyping by sequencing on barley with Ion PGM™ Sequencer Presentation Transcript