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SeedEZ 3D cell culture methods and protocols – tissue culture coating. 3D culturing conditions influence selection and application of coatings for anchorage-dependent cells. Depending on cell types and research objectives, SeedEZ may be coated or uncoated. If coated, SeedEZ may be coated with ligands which promote cell adhesion, or molecules which prevent cell adhesion to the SeedEZ substrate. The former provides a 3D network of cells adhered to and spread inside the SeedEZ. The latter provides an aggregate 3D cell culture model or 3D cell spheroids cultured suspended within the interior of the SeedEZ. Follow our user guidelines to learn which coatings are best suited for your application, whether spheroid cultures or 3D cell cultures of substrate adhered cells. Coating recommendations for diverse 3D cells culture models and detailed coating protocols for bone 3D cell culture models (cultured up to 8 weeks) are provided.