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The Role of Light and Growth Regulators in Seed Germination
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The Role of Light and Growth Regulators in Seed Germination

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This is about the role of phytohormones and light in the germination of seeds.

This is about the role of phytohormones and light in the germination of seeds.


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  • 1. GONZALES-MANOLONG-SOLIJON
  • 2. • A temporary suspension of visible growth of any plant structure containing a meristem (Lang 1987).• After embryo and endosperm development• To initiate germination, three conditions must be met;
  • 3. 1. Must be viable2. Must be subjected to appropriateenvironmental conditions3. Any primary dormancy must be removed
  • 4. • Light has been recognized around the mid- nineteenth century as a critical factor controlling germination (Crocker, 1930).• The photochemically reactive pigment Phytochrome has been shown to be involved in the mechanism of light sensitivity in seeds (Bewley and Black, 1985).
  • 5. • When an imbibed seed is exposed to red light (600-760 nm) phytochrome (P) changes to Pfr which promotes germination.• When exposed to far-red light (760-800 nm), inhibits germination
  • 6. • Specific endogenous plant growth substances have important roles in the control of dormancy.• Most of these are; – Abscisic Acid (ABA) – Gibberellinc Acid (GA) – Cytokinins – Others (Indoleacetic Acid, Kinetin, Auxin)
  • 7. In this experiment the effect of light and certaingrowth regulators on seed germination will beinvestigated.
  • 8. TREATMENTS REPLICATE 1 REPLICATE 2 Germinated Percent Germinated Percent seeds germination seeds germination1.Dark (Control) 10% 4 8% 52. White Light 4 8% 4 8%3. W/ GA3 + Dark 10 20% 10 20%4. w/ IAA + Dark 4 8% 1 2%5. w/ ABA + White 4 8% 1 2%6. Blue Light 0 0 0 07. Green Light 0 0 0 08. Red Light 8 16% 6 12%9. Infrared 1 2% 0 0
  • 9. Red light promotes germinationPhytochrome 660 Phytochrome 730(red absorbing) (far-red absorbing) Far-red light inhibits germinationFigure 1. Effects of red and far-red light on the photochemically reactivepigment phytochrome and lettuce seed germinatio
  • 10. • The membranes of the seed coats and/or the endosperm act as light sensors; once removed light control disappears.
  • 11. Red Light 60 50 40Percent transmittance (%) 30 20 10 0 400 500 600 700 Wavelengths (nm)
  • 12. Blue Light 50 45 40 35Percent Transmittance (%) 30 25 20 15 10 5 0 400 500 600 700 Wavelengths (nm)
  • 13. Green Light 60 50 40Percent Transmittance (%) 30 20 10 0 400 500 600 700 Wavelengths (nm)
  • 14. Far-red Light 4.5 4 3.5 3Percent Transmittance (%) 2.5 2 1.5 1 0.5 0 400 500 600 700 Wavelength (nm)

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