Seminar Schedule <ul><ul><ul><li>1. Vicam products, mycotoxin Tests </li></ul></ul></ul><ul><ul><ul><li>2. IA column vs. E...
Mycotoxin Affinity Column Antibody Bound Resin
Monoclonal Antibody Support Bead
Impurities Mycotoxins
 
Mycotoxins bound to monoclonal antibodies Impurities
Column Wash
Mycotoxins bound to monoclonal antibodies
Methanol elution
Purified mycotoxins in methanol Methanol Mycotoxins
AflaTest <ul><ul><li>Limit of detection as low as 0.5 ppb (fluorometer) 0.05 ppb (HPLC) </li></ul></ul><ul><ul><li>Wide ra...
FumoniTest <ul><ul><li>0.25 ppm (fluorometer) 0.016 ppm (HPLC)  limit of detection </li></ul></ul><ul><ul><li>Wide range o...
ZearalaTest <ul><ul><li>0.10 ppm  (fluorometer)  0.0025 ppm (HPLC) limit of detection </li></ul></ul><ul><ul><li>Wide rang...
OchraTest <ul><ul><li>Limit of detection as low as 1 ppb (fluorometer) 0.25 ppb (HPLC)  </li></ul></ul><ul><ul><li>Wide ra...
DON FQ <ul><li>Quick - Results in less than 30 minutes (after sample extraction. </li></ul><ul><li>Sensitive - Detects lev...
DONtest  HPLC <ul><ul><li>limit of detection  :  0.1 ppm (HPLC)  </li></ul></ul><ul><ul><li>Wide range : 0 -10 ppm </li></...
T-2 <ul><li>Quick - Results in less than 20 minutes (after sample extraction. </li></ul><ul><li>Sensitive - Detects levels...
T-2 HPLC <ul><ul><li>limit of detection  :  0.005 ppm  </li></ul></ul><ul><ul><li>Wide range : 0 –1.5 ppm </li></ul></ul><...
 
 
 
 
 
 
 
 
 
 
Elute AflaToxin with 1.0 ml Methanol Add Developer Solution Measure in Fluorometer Dilute with H 2 O Inject into HPLC
 
 
 
 
 
 
Vicam Immunoaffinity Column vs. ELISA
Training  <ul><li>Vicam IA:   Can be trained in an hour. Concept of method easy to understand. </li></ul><ul><li>ELISA:   ...
Time to run test <ul><li>Vicam IA:  Less than 15 minutes. </li></ul><ul><li>ELISA:   20 minutes or more.  </li></ul><ul><l...
Storage of products <ul><li>Vicam IA:  Room temperature and shelf life up to 1.5 years from date of production. </li></ul>...
Standards <ul><li>Vicam IA:  Reusable standards to calibrate the fluorometer. One time low cost. </li></ul><ul><li>ELISA: ...
Data display <ul><li>Vicam IA:  Fluorometer printout in ppb or ppm for each sample. </li></ul><ul><li>ELISA:   Data printo...
Ordering <ul><li>Vicam IA:  Can order supplies separately as needed. </li></ul><ul><li>ELISA:  Must order as a kit. ELISA ...
Additional Advantage <ul><li>Vicam IA:   </li></ul><ul><li>LOD: 0.5ppb. </li></ul><ul><li>Can be used with an HPLC clean u...
Team Medical & Scientific Sdn. Bhd. 41, Jalan Anggerik Vanilla T31/T, Kota Kemuning,  40460 Shah Alam, Selangor Tel : 603-...
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Vicam Ia Technology Working Principle

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Vicam Ia Technology Working Principle

  1. 2. Seminar Schedule <ul><ul><ul><li>1. Vicam products, mycotoxin Tests </li></ul></ul></ul><ul><ul><ul><li>2. IA column vs. ELISA </li></ul></ul></ul>
  2. 3. Mycotoxin Affinity Column Antibody Bound Resin
  3. 4. Monoclonal Antibody Support Bead
  4. 5. Impurities Mycotoxins
  5. 7. Mycotoxins bound to monoclonal antibodies Impurities
  6. 8. Column Wash
  7. 9. Mycotoxins bound to monoclonal antibodies
  8. 10. Methanol elution
  9. 11. Purified mycotoxins in methanol Methanol Mycotoxins
  10. 12. AflaTest <ul><ul><li>Limit of detection as low as 0.5 ppb (fluorometer) 0.05 ppb (HPLC) </li></ul></ul><ul><ul><li>Wide range as high as 320 ppb </li></ul></ul><ul><ul><li>AOAC Official Method status </li></ul></ul><ul><ul><li>USDA-FGIS Certificate of Conformance </li></ul></ul>
  11. 13. FumoniTest <ul><ul><li>0.25 ppm (fluorometer) 0.016 ppm (HPLC) limit of detection </li></ul></ul><ul><ul><li>Wide range of 0-10 ppm </li></ul></ul><ul><ul><li>Excellent linearity: r = 0.998 </li></ul></ul><ul><ul><li>Excellent precision: %CV < 11% from 1-10 ppm </li></ul></ul><ul><ul><li>Fluorometer method correlates well with HPLC linear regression r = 0.995 </li></ul></ul><ul><ul><li>USDA-FGIS Certificate of Conformance </li></ul></ul>
  12. 14. ZearalaTest <ul><ul><li>0.10 ppm (fluorometer) 0.0025 ppm (HPLC) limit of detection </li></ul></ul><ul><ul><li>Wide range of 0-5 ppm </li></ul></ul><ul><ul><li>Excellent linearity r = 0.999 </li></ul></ul><ul><ul><li>Excellent precision: %CV = 13% over the assay range </li></ul></ul><ul><ul><li>Fluorometer method correlates well with HPLC linear regression r = 0.990 </li></ul></ul>
  13. 15. OchraTest <ul><ul><li>Limit of detection as low as 1 ppb (fluorometer) 0.25 ppb (HPLC) </li></ul></ul><ul><ul><li>Wide range as high as 100 ppb </li></ul></ul><ul><ul><li>Excellent linearity with correlation coefficient (r) of 0.997-0.999 for wheat, dried fruit and green coffee </li></ul></ul><ul><ul><li>Excellent precision with %CV <10% at 20 ppb </li></ul></ul><ul><ul><li>Fluorometer method correlates well with HPLC when analyzed by linear regression </li></ul></ul>
  14. 16. DON FQ <ul><li>Quick - Results in less than 30 minutes (after sample extraction. </li></ul><ul><li>Sensitive - Detects levels as low as 0.5 ppm </li></ul><ul><li>Wide Range - Measures up to 5 ppm </li></ul><ul><li>Convenient - Uses same instrumentation as other VICAM mycotoxin tests </li></ul><ul><li>USDA-FGIS Certificate of Conformance </li></ul>
  15. 17. DONtest HPLC <ul><ul><li>limit of detection : 0.1 ppm (HPLC) </li></ul></ul><ul><ul><li>Wide range : 0 -10 ppm </li></ul></ul><ul><ul><li>Excellent linearity with correlation coefficient (r) of 0.999 : r 2 = 0.999 </li></ul></ul><ul><ul><li>Excellent precision : with %CV 8.3% </li></ul></ul><ul><ul><li>DONtest-HPLC method correlates well with GC-ECD when analyzed by linear regression: r 2 = 0.982 </li></ul></ul>
  16. 18. T-2 <ul><li>Quick - Results in less than 20 minutes (after sample extraction. </li></ul><ul><li>Sensitive - Detects levels as low as 0.15 ppm </li></ul><ul><li>Wide Range - Measures up to 2.0 ppm </li></ul><ul><li>Convenient - Uses same instrumentation as other VICAM mycotoxin tests </li></ul><ul><li>Safe - Requires less toxic materials than other methods. </li></ul>
  17. 19. T-2 HPLC <ul><ul><li>limit of detection : 0.005 ppm </li></ul></ul><ul><ul><li>Wide range : 0 –1.5 ppm </li></ul></ul><ul><ul><li>Excellent linearity with correlation coefficient (r) of 0.999 : r 2 = 0.999 </li></ul></ul><ul><ul><li>Excellent precision : RSD 3.68% </li></ul></ul>
  18. 30. Elute AflaToxin with 1.0 ml Methanol Add Developer Solution Measure in Fluorometer Dilute with H 2 O Inject into HPLC
  19. 37. Vicam Immunoaffinity Column vs. ELISA
  20. 38. Training <ul><li>Vicam IA: Can be trained in an hour. Concept of method easy to understand. </li></ul><ul><li>ELISA: Operator must measure and mix very small volumes, which can bead to errors. Concept of method difficult to understand. </li></ul>
  21. 39. Time to run test <ul><li>Vicam IA: Less than 15 minutes. </li></ul><ul><li>ELISA: 20 minutes or more. </li></ul><ul><li>Test requires 2 incubations of 5 or 10 minutes. </li></ul>
  22. 40. Storage of products <ul><li>Vicam IA: Room temperature and shelf life up to 1.5 years from date of production. </li></ul><ul><li>ELISA: Must be refrigerated and 6 month shelf life. Must throwing away expired product. </li></ul>
  23. 41. Standards <ul><li>Vicam IA: Reusable standards to calibrate the fluorometer. One time low cost. </li></ul><ul><li>ELISA: Must run 3 to 5 standards with every batch. Customer must pay to run each standard, which is costly. </li></ul>
  24. 42. Data display <ul><li>Vicam IA: Fluorometer printout in ppb or ppm for each sample. </li></ul><ul><li>ELISA: Data printout in groups or must be calculated by using a separate computer and special software. </li></ul>
  25. 43. Ordering <ul><li>Vicam IA: Can order supplies separately as needed. </li></ul><ul><li>ELISA: Must order as a kit. ELISA Wells can only be used with reagents in the kit (matched reagents). </li></ul>
  26. 44. Additional Advantage <ul><li>Vicam IA: </li></ul><ul><li>LOD: 0.5ppb. </li></ul><ul><li>Can be used with an HPLC clean up. </li></ul><ul><li>ELISA: </li></ul><ul><li>LOD: 1 - 5 ppb </li></ul><ul><li>Can’t be used for HPLC clean up </li></ul>
  27. 45. Team Medical & Scientific Sdn. Bhd. 41, Jalan Anggerik Vanilla T31/T, Kota Kemuning, 40460 Shah Alam, Selangor Tel : 603-5122 5108 Fax : 603-5122 1608 Email : info@tms-lab.com Blog : tms-lab.blogspot.com Solely Distribute by :

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