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Vicam Ia Technology Working Principle
 

Vicam Ia Technology Working Principle

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    Vicam Ia Technology Working Principle Vicam Ia Technology Working Principle Presentation Transcript

    •  
    • Seminar Schedule
          • 1. Vicam products, mycotoxin Tests
          • 2. IA column vs. ELISA
    • Mycotoxin Affinity Column Antibody Bound Resin
    • Monoclonal Antibody Support Bead
    • Impurities Mycotoxins
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    • Mycotoxins bound to monoclonal antibodies Impurities
    • Column Wash
    • Mycotoxins bound to monoclonal antibodies
    • Methanol elution
    • Purified mycotoxins in methanol Methanol Mycotoxins
    • AflaTest
        • Limit of detection as low as 0.5 ppb (fluorometer) 0.05 ppb (HPLC)
        • Wide range as high as 320 ppb
        • AOAC Official Method status
        • USDA-FGIS Certificate of Conformance
    • FumoniTest
        • 0.25 ppm (fluorometer) 0.016 ppm (HPLC) limit of detection
        • Wide range of 0-10 ppm
        • Excellent linearity: r = 0.998
        • Excellent precision: %CV < 11% from 1-10 ppm
        • Fluorometer method correlates well with HPLC linear regression r = 0.995
        • USDA-FGIS Certificate of Conformance
    • ZearalaTest
        • 0.10 ppm (fluorometer) 0.0025 ppm (HPLC) limit of detection
        • Wide range of 0-5 ppm
        • Excellent linearity r = 0.999
        • Excellent precision: %CV = 13% over the assay range
        • Fluorometer method correlates well with HPLC linear regression r = 0.990
    • OchraTest
        • Limit of detection as low as 1 ppb (fluorometer) 0.25 ppb (HPLC)
        • Wide range as high as 100 ppb
        • Excellent linearity with correlation coefficient (r) of 0.997-0.999 for wheat, dried fruit and green coffee
        • Excellent precision with %CV <10% at 20 ppb
        • Fluorometer method correlates well with HPLC when analyzed by linear regression
    • DON FQ
      • Quick - Results in less than 30 minutes (after sample extraction.
      • Sensitive - Detects levels as low as 0.5 ppm
      • Wide Range - Measures up to 5 ppm
      • Convenient - Uses same instrumentation as other VICAM mycotoxin tests
      • USDA-FGIS Certificate of Conformance
    • DONtest HPLC
        • limit of detection : 0.1 ppm (HPLC)
        • Wide range : 0 -10 ppm
        • Excellent linearity with correlation coefficient (r) of 0.999 : r 2 = 0.999
        • Excellent precision : with %CV 8.3%
        • DONtest-HPLC method correlates well with GC-ECD when analyzed by linear regression: r 2 = 0.982
    • T-2
      • Quick - Results in less than 20 minutes (after sample extraction.
      • Sensitive - Detects levels as low as 0.15 ppm
      • Wide Range - Measures up to 2.0 ppm
      • Convenient - Uses same instrumentation as other VICAM mycotoxin tests
      • Safe - Requires less toxic materials than other methods.
    • T-2 HPLC
        • limit of detection : 0.005 ppm
        • Wide range : 0 –1.5 ppm
        • Excellent linearity with correlation coefficient (r) of 0.999 : r 2 = 0.999
        • Excellent precision : RSD 3.68%
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    • Elute AflaToxin with 1.0 ml Methanol Add Developer Solution Measure in Fluorometer Dilute with H 2 O Inject into HPLC
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    • Vicam Immunoaffinity Column vs. ELISA
    • Training
      • Vicam IA: Can be trained in an hour. Concept of method easy to understand.
      • ELISA: Operator must measure and mix very small volumes, which can bead to errors. Concept of method difficult to understand.
    • Time to run test
      • Vicam IA: Less than 15 minutes.
      • ELISA: 20 minutes or more.
      • Test requires 2 incubations of 5 or 10 minutes.
    • Storage of products
      • Vicam IA: Room temperature and shelf life up to 1.5 years from date of production.
      • ELISA: Must be refrigerated and 6 month shelf life. Must throwing away expired product.
    • Standards
      • Vicam IA: Reusable standards to calibrate the fluorometer. One time low cost.
      • ELISA: Must run 3 to 5 standards with every batch. Customer must pay to run each standard, which is costly.
    • Data display
      • Vicam IA: Fluorometer printout in ppb or ppm for each sample.
      • ELISA: Data printout in groups or must be calculated by using a separate computer and special software.
    • Ordering
      • Vicam IA: Can order supplies separately as needed.
      • ELISA: Must order as a kit. ELISA Wells can only be used with reagents in the kit (matched reagents).
    • Additional Advantage
      • Vicam IA:
      • LOD: 0.5ppb.
      • Can be used with an HPLC clean up.
      • ELISA:
      • LOD: 1 - 5 ppb
      • Can’t be used for HPLC clean up
    • Team Medical & Scientific Sdn. Bhd. 41, Jalan Anggerik Vanilla T31/T, Kota Kemuning, 40460 Shah Alam, Selangor Tel : 603-5122 5108 Fax : 603-5122 1608 Email : info@tms-lab.com Blog : tms-lab.blogspot.com Solely Distribute by :