Plantas medicinales y terapias alternativas en     Alzheimer. Una tierra desconocida Laboratorio de Fisiología de la Repro...
http://www.alz.org/alzheimers_disease_alternative_treatments.asp                  Caprylic acid and coconut oil           ...
Test                     Placebo                  Ginko       Memory                           0,15                     0,...
From: Docosahexaenoic Acid Supplementation and Cognitive Decline in Alzheimer Disease: A RandomizedTrialJAMA. 2010;304(17)...
Donepezil and Memantine for Moderate-to-                               Severe Alzheimers Disease                          ...
Palmer 2002 Trends in Phar
Benilova et al 2012 Nature Neuroscience
Agregados A                 1-40   se localizan en regiones sinápticas                MAP1B – Cy3         A               ...
Parodi et al (2010) JBC
Savonenko et al (2005) Neurobiology of Disease
Dinamarca et al (2006) Molecular Psychiatry
Inestrosa et al (2011) Translation Psychiatry
Montesinos et al (2012) in report
1,2                                                                  Control                                              ...
A   BC   D
A                                         B                        LYS-28                                  LYS-28         ...
A                                                     B    12                                                             ...
2,5                                                             Actividad Sináptica Espontánea                            ...
A                                                                       B                                                 ...
A                                                                                  B              Control                 ...
NR                                              15 min Aggregate             0 min                    15 min100 μM ATP    ...
AgradecimientosDr. Luis AguayoDr. Nibaldo InestrosaFernando SepulvedaDavid OrmeñoLaboratorio de Fisiología de la Reproducc...
Presentación nucleo uct 2012 ii
Presentación nucleo uct 2012 ii
Presentación nucleo uct 2012 ii
Presentación nucleo uct 2012 ii
Presentación nucleo uct 2012 ii
Presentación nucleo uct 2012 ii
Presentación nucleo uct 2012 ii
Presentación nucleo uct 2012 ii
Presentación nucleo uct 2012 ii
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Presentación nucleo uct 2012 ii

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Presentación nucleo uct 2012 ii

  1. 1. Plantas medicinales y terapias alternativas en Alzheimer. Una tierra desconocida Laboratorio de Fisiología de la Reproducción Jorge Parodi jparodi@uct.cl
  2. 2. http://www.alz.org/alzheimers_disease_alternative_treatments.asp Caprylic acid and coconut oil Concerns Coenzyme Q10 Coral calcium Ginkgo biloba Huperzine A Omega-3 fatty acids Phosphatidylserine Tramiprosate
  3. 3. Test Placebo Ginko Memory 0,15 0,14 Executive test 3,27 3,47 Conclusion Compared with placebo, the use of G biloba, 120 mg twice daily, did not result in less cognitive decline in older adults with normal cognition or with mild cognitive impairment.Snitz et al 2009 JAMA
  4. 4. From: Docosahexaenoic Acid Supplementation and Cognitive Decline in Alzheimer Disease: A RandomizedTrialJAMA. 2010;304(17):1903-1911. doi:10.1001/jama.2010.1510 Copyright © 2012 American MedicalDate of download: 7/14/2012 Association. All rights reserved.
  5. 5. Donepezil and Memantine for Moderate-to- Severe Alzheimers Disease Conclusions In patients with moderate or severe Alzheimers disease, continued treatment with donepezil was associated with cognitive benefits that exceeded the minimum clinically important difference and with significant functional benefits over the course of 12 months JAMA 2012Danitz and Parsons 2008 IJNP
  6. 6. Palmer 2002 Trends in Phar
  7. 7. Benilova et al 2012 Nature Neuroscience
  8. 8. Agregados A 1-40 se localizan en regiones sinápticas MAP1B – Cy3 A DA – DAC (1:2)24 horas H0.5 M A - DAC B G SV2 – Cy5 C G H 10 m
  9. 9. Parodi et al (2010) JBC
  10. 10. Savonenko et al (2005) Neurobiology of Disease
  11. 11. Dinamarca et al (2006) Molecular Psychiatry
  12. 12. Inestrosa et al (2011) Translation Psychiatry
  13. 13. Montesinos et al (2012) in report
  14. 14. 1,2 Control 5 mM Etanol 1,0 10 mM Etanol A B 50 mM Etanol (D.O. 405 nm) Turbimetria 0,8 0,6 0,4 0,2 0,0 0 50 100 150 200 Tiempo (min) C D Modeling EOrmeño et al (2012) in review
  15. 15. A BC D
  16. 16. A B LYS-28 LYS-28 2.7Ẳ ASP-23 ASP-23C LYS-28 D 12.7Ẳ LYS-28 6.3Ẳ ASP-23 ASP-23
  17. 17. A B 12 25 Control Control Ethanol 10 % Ethanol 1% 10 20 Deviation (RMSD) 8 15Ẳ 6 10 4 5 2 0 0 0,0 0,5 1,0 1,5 2,0 2,5 0,0 0,5 1,0 1,5 2,0 2,5 Time (ns) Time (ns)
  18. 18. 2,5 Actividad Sináptica Espontánea Agregados amilodes 2,0 Frecuencia (Hz) 1,5 * 1,0 * Control * 0,5 20 pA * 0.2 s 0,0 Agregados A 0.5 M (24 h) 10 25 50 0 1 5 as ol 10 tr ad on 20 pA at Etanol (mM) C Tr 0.2 s Agregados A 0.5 M + 10 mM Etanol (24 h) 20 pA 0.2 s Agregados A 0.5 M + 50 mM Etanol (24 h) Sv2 20 pA 0.2 s 50 mM Etanol (24 h) 20 pA 0.2 s A aggregates 0.5 MOrmeño et al (2012) in review
  19. 19. A B C 8 1,5 -1000 Aggregate Membrane conductances (nS) Control Gramicidin Gramicidin 6Number of events Amphotericin B Amphotericin Aggregate -800 * Control 4 1,0 * -600 * 2 mV 0 -400 -200 -150 -100 -50 0 50 100 0,5 -2 -200 -4 0,0 0 -6 -6 -5 -4 -3 -2 -1 0 1 s ol in B te nA tr id in Amplitude (nA) ga on ic ic -8 re m er C ra gg ot G ph A m AParodi et al (2012) in review
  20. 20. A B Control 300 (% of control at 20 mV) High Ca2+ * Current amplitude NR 250 + 20 mV 0 mV + 200 -100 mV * 150 * Aggregate 100 nA 250 ms 100 High Ca2+ NR 50 + 20 mV 0 mV 0 -100 mV NR High Ca2+ NR High Ca2+ Aggregate 200 C D Current amplitude * (% of control ) NR 15 min Aggregate 150 NR 0 min 15 minRabbit serum 100 50 200 nA 0 4 min 0 min 15 min 15 min Aggregate
  21. 21. NR 15 min Aggregate 0 min 15 min100 μM ATP 120 Current (% of control) 100 80 60 40 * 200 nA 20 0 60 s 0 min 15 min 15 min Aggregate
  22. 22. AgradecimientosDr. Luis AguayoDr. Nibaldo InestrosaFernando SepulvedaDavid OrmeñoLaboratorio de Fisiología de la ReproducciónFelipeGuillermoDanielaColaboradoresDr. Alexander OrtloffMsC Patricio Peña

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