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  • 1. -Its a type of electromagnetic spectroscopy which analyze fluorescence from a sample. - it involves using abeam of light , usually ultraviolet light thatexcites the electron in molecule ofcertain compound and causes them to emit light of a lower energy
  • 2. CONCENTRATION AND FLUORESCENCE INTENSITY : The power of fluorescent radiation, F, is proportional to theradiant power of the excitation beam absorbed by the species able to undergo fluorescence: F = K(P0 - P) where P0 is the power incident on the sample, P isthe power after it traverses a length b of the solution and K is a constant which depends upon experimental factors and the quantum efficiency of fluorescence. Beers law can be rearranged to give: P/P0 = 10- bc where A =  is the absorbance. bc Substitution gives: F = KP0(1 - 10- bc) This is the fluorescence law Unlike Beer’s Law fluorescence isn’t in general linear with concentration This expression can be expanded (Taylor series): To a good approximation if  is small (< 0.05) the higher- bc order terms are nearly zero, we have: F = 2.3KbcP0
  • 3. -which demonstrates twoimportant points:1-that at low concentrationsfluorescence intensity isproportional to concentration;-at the absorption frequencyFor a concentration above c1the calibration curve is nolonger linear2-that fluorescence isproportional to the incidentpower in the incident radiation
  • 4. http://en.wikipedia.org/wiki/Fluorescence_spectroscop y http://medical- dictionary.thefreedictionary.com/fluorometry http://www.answers.com/topic/fluorometryhttp://www.google.com.eg/search?q=Fluorometry&hl=ar&gbv=2&prmd=imvnsb&tbm=isch&tbo=u&source=univ&sa=X&ei=iwbXTsiBCcmv8gOtoOjMDQ&ved=0CJMBELAE&biw= 1280&bih=656
  • 5. 1- Aya Ahmed Saber Yosif 86 2- Jehan Essam Mahmoud 1123- Eman Mohammed Mostafa 834- Basma Sayed Saeed Ahmed5- Bosy Nabil6- Ayat Fwzy7- Eman Gamal8- Gourge Reda9- Andro Esmat10- Jhon Esmat