Ask a Biosafety Expert Service at ABSA 2013

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A description of DIYbio.org's Ask a Biosafety Expert (ABE) service, as it was presented at the annual ABSA meeting in Kansas City on October 23, 2013.

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  • {"12":"From Left to Right, Top to Bottom\nPearl Biotech, GelBox\nMFC\nOpenPCR\nDremelfuge\n(5) LavaAmp\n","8":"(1) A cancer biotech company incubated its business in a garage in Mountainview, although they have since vacated it and moved to a commercial facility. (2) Kay Aull built a small lab in her bedroom closet in Cambridge, MA and used it to perform allele-specific genotyping for HFE, a disease that runs in her family. (3) Meredith Patterson working from her kitchen in the Bay Area on a project to engineer yogurt bacteria to sense the food contaminant, melamine. (4) The start-up company OTYP, an educational biotechnology business, ran an R&D lab out of the founder’s college apartment in Ann Arbor.\nhttp://www.flickr.com/photos/sugargliders/4561317461/in/pool-1296598@N22/\nhttp://www.boston.com/news/science/articles/2009/05/11/do_it_yourself_genetic_sleuthing/\nhttp://www.msnbc.msn.com/id/28390773/ns/technology_and_science-innovation/\nhttp://www.biotechniques.com/news/biotechniquesNews/biotechniques-301745.html\n","3":"Issues that a Biosafety Professional Sees Here:\n1. Unlabeled, unknown solutions/chemicals\n2. Potential electrical hazard\nIs this thermometer filled with mercury?\nUsing a household air purifier – to attempt to control what kind of hazard? Chemical fumes = won’t work b/c HEPA filters won’t capture vapors only particles\nOutdated (unsafe?) centrifuge\nHeat block if left on at high temperature = fire hazard, especially if lab is in garage with paint/solvents/gasoline stored nearby.\n","10":"San Francisco community lab – membership to use lab and equipment\nBuy your own reagents\nSafety training and intro courses\n"}
  • Ask a Biosafety Expert Service at ABSA 2013

    1. 1. October 23, 2013 Ask a Biosafety Expert: User-driven advisory service for the Do-It-Yourself Biology (DIYbio) Community ABSA Annual Meeting Kansas City, MO Jason Bobe Co-founder, DIYbio.org jason@diybio.org twitter:@jasonbobe 1
    2. 2. New Teen Hobby: DIY DNA labs Photo credit: Fred Turner, 2013 UK Young Engineer of the Year
    3. 3. New Teen Hobby: DIY DNA labs Photo credit: Fred Turner, 2013 UK Young Engineer of the Year
    4. 4. Dawn of the Biohackers. Discover Magazine. Oct 5, 2011. Photo by Grant Delin
    5. 5. Vibrant Hobby Communities CHEMISTRY Lab Technician Set for Girls c. 1958 PERSONAL COMPUTING Homebrew Computer Club September 1976 ASTRONOMY Hanny’s Voorwerp discovered August 13, 2007 ROCKETRY Amateur Saturn V launch in MD April 25, 2009 Credits: Chemical Heritage Foundation; DigiBarn Computer Museum; Sloan Digital Sky Survey; Dick Stafford, RocketryPlanet.com.
    6. 6. Biology is Next: Global DIYbio Community NORTH AMERICA Atlanta Baltimore Boston Brooklyn Cambridge Carlsbad Chicago Houston Los Angeles Nashville New York City Oakland Portland San Diego San Francisco Seattle Sunnyvale Toronto Vancouver Victoria EUROPE Budapest Copenhagen Cork Eindhoven Graz Groningen Kiev Lausanne London Manchester Munich Namur The Hague Paris Prague Switzerland ASIA Singapore Tel-Aviv OCEANIA Auckland Sydney
    7. 7. Scope of Activities Exploratory Biology Environmental Sensing Personal BioMonitoring Constructive Biology Re-imagining Laboratory Devices Genetic Engineering 7
    8. 8. Home Workshops 1.Garage: cancer biotech start-up 2.Closet: Personal genotyping facility 3.Kitchen: yogurt engineered to sense melamine (toxin) 4.Dorm: R&D lab for low-cost PCR machine
    9. 9. Community Labs Genspace (Brooklyn, NY) NYTimes. December 19, 2010. Photo Michael Nagle
    10. 10. Community Labs BioCurious (Sunnyvale, CA) http://biocuriosity.files.wordpress.com/2011/07/welcome-to-biocurious-sign.jpg?w=630 http://biocuriosity.files.wordpress.com/2011/09/img_0551.jpg?w=630
    11. 11. DIY BioPrinter Biocurious Project: BioPrinter 6/ 2/ 13 2:50 PM (/files/deriv/FK7/UUYW/HAQ3D3X1/FK7UUYWHAQ3D3X1.LARGE.jpg) http://www.instructables.com/id/DIY-BioPrinter/ (/files/deriv/F8C/V1Y8/HBXWRTDL/F8CV1Y8HBXWRTDL.LARGE.jpg) (/files/deriv/F7P/LTPM/HAQ3D3Z2/F7PLTPMHAQ3D3Z2.LARGE.jpg) http://www.technologyreview.com/sites/default/files/styles/view_body_embed/p http://www.technologyreview.com/sites/default/files/styles/view_body_embed/p ublic/images/bioprinter_0.jpg?itok=ZkO-8Tay ublic/images/biocurious.jpg?itok=CAGwqHG9
    12. 12. Innovation Engine: Small Start-Ups Creating Low-Cost Kits & Devices --> Crowdsourcing $$$ Open GelBox 2.0 Microbial Fuel Cell Dremelfuge OpenPCR LavaAmp
    13. 13. iGEM: International Genetically Engineered Machines Competition • 3200 active members • 215 teams & 200 labs • Sections http://2012.igem.org/wiki/images/6/65/Biobrick_trophy.jpg http://www.flickr.com//photos/igemhq/sets/72157625225590751/show/ – – – – High school Undergraduate Overgraduate DIY / Community Lab section planned for 2014
    14. 14. Ask a Biosafety Expert Outreach, education, and good, practical biosafety advice http://ask.diybio.org
    15. 15. Some of the questions so far…
    16. 16. How can I tell if the bacteria that I’m growing on agar plates in my room are toxic?
    17. 17. How should I dispose of my bacterial plates if I don’t have access to an autoclave?
    18. 18. How can I make bioart pieces with embedded non-pathogenic bacteria safe to sell to the public?
    19. 19. Real Opportunities for Biosafety Professionals • Movement is growing rapidly, but is still relatively small • An opportunity to build in safety culture from the earliest stages • Challenging questions, new technology, new contexts, new types of clients, open access resources
    20. 20. Call for Volunteers please join our expert advisory board •Required training and experience – – – – All levels RBP and CBSP highly desirable Different roles for different levels of training, experience, certification Need diverse mix of domain expertise •Minimum volunteer commitments – – – respond to 1 question per month Monthly 1 hour conference call ~5 hours per month •About the Organization – – – DIYbio.org is nonprofit corporation ABE is a free educational service Insured
    21. 21. BIG THANKS TO OUR CURRENT ADVISORY BOARD MEMBERS Molly Stitt-Fischer, Ph.D., CPH. Ted Myatt, Sc.D., RBP. J. Craig Reed, Ph.D., RBP. Special thanks to Woodrow Wilson Center for International Scholars, Synthetic Biology Project, Dave Rejeski and Todd Kuiken. Email: jason@diybio.org Twitter: @jasonbobe 21
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