Effect of Varying pH on therelease of Betacyanin from Beta vulgaris root cells Brittney Fiselier and Kierra Walker Red Deer College - Red Deer
Introduction• If the pH of the surrounding environment of a Beta vulgaris beet root is lowered, then more red pigment, betacyanin, will be released because more cell damage will be done.• The variation that was presented to the Beta vulgaris root cells was varying pH levels of 2.0, 7.7 and 12.0.• According to Reid et al, who studied the effects of pH on betacyanin leakage from beet root discs, the lower the pH, the more pigment is released.• According to Jacobsohn et al, who studied cell membranes and the inﬂuence of pH on solvent induced damage, betacyanin is released at its maximum at a pH of 2.0 and little leakage at pH of 6.6 and 10.0.• According to Thimmaraju et al, who studied the kinetics of pigment release from hairy root cultures of Beta vulgaris under the inﬂuence of pH, sonication, temperature and oxygen stress, about 70% of the betacyanin was obtained within 30 mins when the beet root was placed in an acidic medium of pH 2.0.
Methods Ten 5 mm discs of a sample of a Beta vulgaris beet root was obtained and rinsed for 10 mins. Nine test tubes were labelled three of HCl, three of NaOH, and the remaining three of H20. Asample size of 3 was used and three discs were placed in each solution for exactly 2 mins. Each disc sample was placed in the appropriate test tube containing distilled water for 30 mins,being vortexed every 10 mins. Using a pipette, 10 mL of each sample was obtained and placed in a cuvette which was used in the Spectrophotometer 20 to measure the absorbency at a wavelength of 525 nm.Our control was the sample of pH of 7.7 (distilled water). This mimics the natural pH range of a Beta vulgaris beet root.
Discussion• Our hypothesis was supported by our results; Beta vulgaris membrane cells are damaged when the pH of its environment decreases.• It can be concluded that Beta vulgaris beet roots would not grow as healthy in low pH soil because their membrane cells would not be able to withstand the acidity, causing damage to the central vacuole which contains the red pigment, betacyanin.• Experimental errors include the precision of the disc samples of the beet root because a non-scientiﬁc ruler was used to measure 5 mm, misreadings of the Spectrophotometer 20 could have been incorrect if careful measures were not taken by using the mirror behind the needle and the blank sample used to calibrate the Spectrophotometer may have contained some betacyanin on the outside of the cuvette.• Modiﬁcations to incorporate could be to take care in more precision when taking any measurements and to test more variations of pH ranges to get more exact results.