Poster 54 hematologie

2011 Hematologie Hematology Poster 54 PREVALENCE OF HAEMOGLOBIN VARIANTS IN GREEK PATIENTS Marilena Stamouli , Anastasios Skliris, Grigorios Totos, Dimitrios Kokoulas and Efrosini Karagianni Department of Microbiology and Immunology, Naval and Veterans Hospital, Athens, Greece

Hematologie Hematology Poster 54
Hemoglobinopathies are inherited disorders of hemoglobin synthesis or of its structure, which cause significant morbidity and mortality worldwide
The prevalence of carriers of abnormal hemoglobin is within the range of 5 %-7 % globally
the number of new cases of affected infants is estimated at 300000.0 per year
The aim of this study was the estimation of the prevalence of hemoglobin variants in Greek patients .
Facts and Objectives 2011

2011 Hematologie Hematology Poster 54
The present study was conducted on 875 blood samples derived from patients:
aged between 3 and 86 years
born in different regions of Greece.
510 (58 %) samples were males and 376 (42 %) were females.
Participants were hospital inpatients and outpatients, and candidates for diving certification .
Materials and Methods (I)

2011 Hematologie Hematology Poster 54
Venous blood samples were collected by venipuncture into K + EDTA anticoagulated tubes. Sample evaluation included:
measurement of total Hb, Hct, RBC, MCH, MCV, RDW
sickle tests
iron and ferritin status
Identification and quantification of hemoglobins was carried out by High Performance Liquid Chromatography (HPLC) on the MENARINI HA-8160 automated analyzer.
HPLC is suitable for the simultaneous detection and quantification of hemoglobin fractions.
Abnormal cases were confirmed by hemoglobin electrophoresis at pH 8.6 on the SEBIA Hydrasys fully automated electrophoresis system.
Materials and Methods (II)

Out of the 875 patients:
660 (86.86 %) had normal HbA, HbA 2 and HbF levels, and
115 (13.14 %) had at least one abnormal such level.
2011 Hematologie Hematology Poster 54 Results (I)

98 (11.2 %) patients had β -thalassaemia trait with elevated HbA 2 levels,
8 patients (0.91%) had sickle cell trait,
7 patients (0.8%) had both β -thalassaemia and sickle cell traits, and
2 patients (0,22 %) had hereditary persistence of fetal hemoglobin, presenting HbF levels of 15% and 30 % respectively
2011 Hematologie Hematology Poster 54 Results (II)

The hemoglobinopathies constitute a major public health problem, because of their high frequency and clinical severity.
Greece is a country with considerable frequency of both β -thalassemia and sickle cell traits.
Our finding of a prevalence of 11.2 % for the β -thalassemia trait and 0.91 % for sickle cell trait is consistent with previous reports.
In national scale studies, the prevalence range of β -thalassemia trait was found 5 % to 15 % and the prevalence range of sickle cell trait was found 1 %;
considerable regional differences have been observed.
2011 Hematologie Hematology Poster 54 Discussion

Hemoglobinopathies are now encountered in most countries of the world as a result of global population movements and their distribution changes continuously over the years.
In the future there will be a strong need for collaboration on information strategies, screening and genetic counseling among countries, in order to deal effectively with this serious health problem.

2011 Hematologie Hematology Poster 54 Concluding Remarks

Poster 54 hematologie

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