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Poster 45 biochimie

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RAPID MEASUREMENT OF

RAPID MEASUREMENT OF
H-FABP IN SERUM WITH AN IMMUNOTURBIDIMETRIC
ASSAY KIT

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    Poster 45 biochimie Poster 45 biochimie Presentation Transcript

    • 2011 Biochimie Biochemistry Poster 45 RAPID MEASUREMENT OF H-FABP IN SERUM WITH AN IMMUNOTURBIDIMETRIC ASSAY KIT Piper, K., Lowry, P., Gilmore, K., Szabo, M., Rachidi, P., McConnell, R.I., Campbell, J., Lamont, J.V., Fitzgerald, S.P. Randox Laboratories Ltd., Crumlin, United Kingdom
      • INTRODUCTION
      • Heart-type fatty acid-binding protein (H-FABP) is a small cytoplasmic protein, involved in
      • fatty acid metabolism. It is abundant in heart muscle and is an early, highly sensitive
      • marker of myocardial ischemia. H-FABP has been shown to aid in the diagnosis of acute
      • myocardial infarction complementing cardiac troponin I as it is elevated at a much earlier
      • time point. As such, its measurement provides early diagnostic and prognostic
      • information, which is relevant for patient management.
      •  
      • Analytical methods enabling the rapid detection of H-FABP in biological samples are
      • advantageous in clinical settings where a short time span for the assessment is critical.
      • This study reports an immunoturbidimetric assay kit applicable to automated analyzers
      • for the rapid measurement of H-FABP in serum .
      • METHODOLOGY
      • Principle
      • H-FABP assay
      • The principle of the assay is immunoturbidimetric.
      • A latex agglutination complex is formed between H-FABP
      • and antibody coated latex particles
      • The assay kit (FB 4025, Randox Laboratories, Crumlin, UK) was applied to different
      • automated analyzers and compared with another commercially available biochip array
      • based assay on the Evidence Investigator analyser
      • (EV3602, Randox Laboratories Ltd., Crumlin, UK).
      • Sample
      • Serum
      • Reagents and sample volumes
      • Liquid reagents ready for use
      • RESULTS
      • H-FABP immunoturbidimetric assay:
      • Performance outline on RX series analysers
      Precision Assay range* ng/ml 2.94 - 123 H-FABP Concentration ng/ml Intra-assay precision %CV Level 1(n = 20) 5.98 8.03 Level 2 (n = 20) 30.8 2.08 Level 3 (n = 20) 91.3 1.93
      • Comparison with commercially available H-FABP assay on biochip array platform
      •  
      • H-FABP: RX series analysers (Daytona)/Biochip array platform (on Evidence Investigator analyser)
      Y = 1.19X - 1.370 r = 0.976 n=53
      • H-FABP immunoturbidimetric assay:
      • Performance outline on Advia 1650
      Precision Assay range* ng/ml 0.747-120 H-FABP Concentration ng/ml Intra-assay precision %CV Level 1(n = 20) 5.75 7.94 Level 2 (n = 20) 40.3 1.33 Level 3 (n = 20) 91.9 1.47
    • *99th Percentile cut-off 6.32 ng/ml H-FABP measurement in patient samples on a Konelab analyzer Sample collection interval after first chest pain 1-3 hours n=19 Sample ID Within 1 hour Sample ID Within 3 hours H-FABP (ng/ml)* Trop I (ug/L ) H-FABP (ng/ml)* Trop I (ug/L) 1 3.71 <0.04 5 44.04 1.1 2 6.46 <0.04 6 5.03 <0.04 3 12.97 <0.04 7 6.51 <0.04 4 3.86 <0.04 8 3.05 <0.04 9 3.48 <0.04 10 5.61 <0.04 11 48.94 1.1 12 12.35 <0.04 13 5.14 <0.04 14 9.81 <0.04 15 5.82 <0.04 16 5.76 <0.04 17 12.52 <0.04 18 12.55 <0.04 19 7.87 <0.04
      • CONCLUSION
      • The data indicates that this immunoturbidimetric assay kit for the measurement
      • of H-FABP is applicable to automated analyzers and facilitates the rapid
      • assessment of early myocardial ischemia in clinical settings.
      • Acknowledgment
      • Authors would like to thank Mr. W. Shaw, Withybush General Hospital, U.K. and Ms. N. James, University of Wales, U.K. for the sample analysis on the Konelab analyzer.