Your SlideShare is downloading. ×
0
Gas Chromatography
Compiled by : Imanuelle Orchidea
3325102413
Definition
• A chromatographic technique in which the
mobile phase is a gas
Gas

Gas –Liquid/
Gas-Solid
Chromatography
Liq...
Rangkaian
penghubung &
penguat

Tabung gas

perekam
Mobile phase
• Inert gas ( He, Ar, N2)
• Carry the sample
• Flow rates determined with a flow meter
placed at a column out...
Packed columns

Capillary columns
Packed Columns
– Glass/stainless steel/copper/aluminum
– 2-6 m in length, internal Ø 2-4mm
– Particle Ø ranging from 37-44...
Capillary Columns
• Open tubular column
– Wall-coated
– Support-coated

• Fused silica coated with protective polymer
• Up...
• Photo of capillary column, taken from Harvey,
David .T. Modern Analytical Chemistry
Stationary phase
• Choice of stat. phase  selectivity
• Criteria :
– CHEMICALLY INERT
– THERMALLY STABLE
– LOW VOLATILITY...
STAT.PHASE

POLARITY

TRADE NAME (T)LIMIT

APPLICATIONS

Squalane

Non-polar

Squalane

150

Low boiling aliphatic hydroca...
Introduction of samples
1. All constituents must be volatile
2. Appropriate concentration
3. Must not degrade the separati...
Injecting the sample
• Split injection
• Splitless injection
• On-column injection

Schematic diagram of an injector
for p...
Detectors in GC

Thermal Conductivity
Another detectors
•
•
•
•

Flame photometric detector
Thermionic detector
FT-IR (GC-FT-IR)
MS (GC-MS)
Quantitative applications
•
•
•
•

Environmental analysis
Clinical analysis/forensic may include
Consumer goods
Petroleum ...
Gas chromatography
Gas chromatography
Gas chromatography
Upcoming SlideShare
Loading in...5
×

Gas chromatography

493

Published on

0 Comments
1 Like
Statistics
Notes
  • Be the first to comment

No Downloads
Views
Total Views
493
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
21
Comments
0
Likes
1
Embeds 0
No embeds

No notes for slide
  • Split injection :dipakaikalosampelkadarnyatinggi, splitlessdipakaikalosampelkadarnyarendahbangetdanbutuhdipekatkandikit, on column kalosampelmudahrusakpadasuhutinggi
  • Transcript of "Gas chromatography"

    1. 1. Gas Chromatography Compiled by : Imanuelle Orchidea 3325102413
    2. 2. Definition • A chromatographic technique in which the mobile phase is a gas Gas Gas –Liquid/ Gas-Solid Chromatography Liquid Solid
    3. 3. Rangkaian penghubung & penguat Tabung gas perekam
    4. 4. Mobile phase • Inert gas ( He, Ar, N2) • Carry the sample • Flow rates determined with a flow meter placed at a column outlet Column type Flow rate Packed column 25-150 mL/min Capillary column 1-25 mL/min
    5. 5. Packed columns Capillary columns
    6. 6. Packed Columns – Glass/stainless steel/copper/aluminum – 2-6 m in length, internal Ø 2-4mm – Particle Ø ranging from 37-44 µm to 250-354 µm – diatomaceous earth, glass beads, fluorocarbons polymers – Handle larger samples – Pressure needed to move mobile phase limits it’s length
    7. 7. Capillary Columns • Open tubular column – Wall-coated – Support-coated • Fused silica coated with protective polymer • Up to 100 m in length • Internal Ø 150-300 µm, megabore up to 530 µm • Handle smaller samples ( < 10-2 µL)
    8. 8. • Photo of capillary column, taken from Harvey, David .T. Modern Analytical Chemistry
    9. 9. Stationary phase • Choice of stat. phase  selectivity • Criteria : – CHEMICALLY INERT – THERMALLY STABLE – LOW VOLATILITY – APPROPRIATE POLARITY • R – SiR2 – O - (SiR2 – O)n – SiR3 • HO – CH2 – CH2 – (O – CH2 – CH2)n – OH
    10. 10. STAT.PHASE POLARITY TRADE NAME (T)LIMIT APPLICATIONS Squalane Non-polar Squalane 150 Low boiling aliphatic hydrocarbons Apezion L Non-polar Apezion L 300 Amides Fatty acid methyl esters High boiling aliphatic HC Terpenoids Polydimethyl siloxane Slightly SE-30 300-350 Alkaloids* Amino acid derivatives ** Drugs# pesticides## Phenols & steroids 50% methyl50%phenyl polysiloxane Moderate OV-17 375 *, #, ##, Polyaromatic hydrocarbons Polychlorinated biphenyls 50%TFP50%MPSO Moderate OV-210 275 *,**, #, halogenated compounds, ketones, phenols 50% CYP-50% phe-me-PSO Polar OV-225 275 Nitriles, pesticides, steroids PEG Polar Carbowax 20M 225 Aldehydes, esters, ethers, phenols
    11. 11. Introduction of samples 1. All constituents must be volatile 2. Appropriate concentration 3. Must not degrade the separation Volatile analyte from Non volatile matrix: 1. 2. 3. 4. Liquid-liquid extraction Solid phase extraction  SPME Headspace sampling Cryogenic focusing
    12. 12. Injecting the sample • Split injection • Splitless injection • On-column injection Schematic diagram of an injector for packed column GC
    13. 13. Detectors in GC Thermal Conductivity
    14. 14. Another detectors • • • • Flame photometric detector Thermionic detector FT-IR (GC-FT-IR) MS (GC-MS)
    15. 15. Quantitative applications • • • • Environmental analysis Clinical analysis/forensic may include Consumer goods Petroleum industry
    1. A particular slide catching your eye?

      Clipping is a handy way to collect important slides you want to go back to later.

    ×