Differential Diagnosis ofBrucellosis Serologic ReactionsSteven G. HennagerBacterial and Hemoparasitic Serology TeamNational Veterinary Services LaboratoriesWorkshop: An Integrated Approach to Controlling Brucellosis in Africa, AddisAbaba, 29-31 January 2013Safeguarding Animal Health
Dominant Brucella AntigensSpecies• Brucella melitensis• Brucella abortus• Brucella suis• Brucella canis• Brucella ovisPrimary Antigen• M and A• A and M• A and M• Rough• Rough
Safeguarding Animal HealthImmune response to Brucella infection• Cell mediated response• Humoral or antibody production
Safeguarding Animal HealthType: JPGWebsite for this image... and faster response - antibody titre is much higher, occurs much faster, ...classes.midlandstech.edu•Full-size image•760 × 600 (1.2x larger), 66KB•More sizes•Search by image•Similar imagesImages may be subject to copyright.
Safeguarding Animal HealthSerologic Assays Antibody detection Serum Milk Rapid Inexpensive Sensitivity and Specificity Assays can be impacted by Vaccination Environmental conditionsBrucella cardtest
Brief History 19th century – Bruce isolated organismfrom human spleens Linked organism to raw goat’s milk Genus named after him 19th century – Bang discoveredorganism that caused abortions incattle Named organism Bacillus abortus 20th century – Evans recognized therelationship between Bruce’s organismand Bang’s organism Advocated pasteurization of milk Re-named organism Brucella abortusSir DavidBruceL.F. BernhardBangAlice C. Evans
Acknowledge Superior LaboratorySupport• De Anne Wall, Diane Davis, BarbKesl, Molly Fritz– Serology Laboratory TechniciansQuestions?