Differential diagnosis of brucellosis serologic reactions

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Presented by Steven G. Hennager at a workshop on an integrated approach to controlling brucellosis in Africa, Addis Ababa, Ethiopia, 29-31 January 2013.

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Differential diagnosis of brucellosis serologic reactions

  1. 1. Differential Diagnosis ofBrucellosis Serologic ReactionsSteven G. HennagerBacterial and Hemoparasitic Serology TeamNational Veterinary Services LaboratoriesWorkshop: An Integrated Approach to Controlling Brucellosis in Africa, AddisAbaba, 29-31 January 2013Safeguarding Animal Health
  2. 2. Dominant Brucella AntigensSpecies• Brucella melitensis• Brucella abortus• Brucella suis• Brucella canis• Brucella ovisPrimary Antigen• M and A• A and M• A and M• Rough• Rough
  3. 3. Blue colonies = Smooth Brucella sp.Crystal colonies = Rough Brucella sp.
  4. 4. Safeguarding Animal HealthImmune response to Brucella infection• Cell mediated response• Humoral or antibody production
  5. 5. Safeguarding Animal HealthType: JPGWebsite for this image... and faster response - antibody titre is much higher, occurs much faster, ...classes.midlandstech.edu•Full-size image•760 × 600 (1.2x larger), 66KB•More sizes•Search by image•Similar imagesImages may be subject to copyright.
  6. 6. Safeguarding Animal HealthSerologic Assays Antibody detection Serum Milk Rapid Inexpensive Sensitivity and Specificity Assays can be impacted by Vaccination Environmental conditionsBrucella cardtest
  7. 7. Brucellosis Diagnostic Tests
  8. 8. Brucellosis Diagnostic Tests
  9. 9. Safeguarding Animal HealthBrucellosis Diagnostic Tests
  10. 10. Brucellosis Diagnostic Tests
  11. 11. Brucellosis Diagnostic Tests
  12. 12. Brucellosis Diagnostic Tests
  13. 13. Other Brucella Diagnostic Tests• Polymerase Chain Reaction (PCR)• Culture Identification• Western Blot
  14. 14. Amount of Isotype (ng)Test IgM IgG1 IgG2 IgATube 20 ----- 125 650Card ----- 600 7500 -----Riv ----- 1550 2750 -----CF 6500 290 ----- -----ELISA 155 190 220 700
  15. 15. Brief History 19th century – Bruce isolated organismfrom human spleens Linked organism to raw goat’s milk Genus named after him 19th century – Bang discoveredorganism that caused abortions incattle Named organism Bacillus abortus 20th century – Evans recognized therelationship between Bruce’s organismand Bang’s organism Advocated pasteurization of milk Re-named organism Brucella abortusSir DavidBruceL.F. BernhardBangAlice C. Evans
  16. 16. Acknowledge Superior LaboratorySupport• De Anne Wall, Diane Davis, BarbKesl, Molly Fritz– Serology Laboratory TechniciansQuestions?

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