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Differential diagnosis of brucellosis serologic reactions

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Presented by Steven G. Hennager at a Workshop: An Integrated Approach to Controlling Brucellosis in Africa, Addis Ababa, 29-31 January 2013 …

Presented by Steven G. Hennager at a Workshop: An Integrated Approach to Controlling Brucellosis in Africa, Addis Ababa, 29-31 January 2013

Published in: Technology, Business

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  • 1. Differential Diagnosis ofBrucellosis Serologic ReactionsSteven G. HennagerBacterial and Hemoparasitic Serology TeamNational Veterinary Services LaboratoriesWorkshop: An Integrated Approach to Controlling Brucellosis in Africa, AddisAbaba, 29-31 January 2013Safeguarding Animal Health
  • 2. Dominant Brucella AntigensSpecies• Brucella melitensis• Brucella abortus• Brucella suis• Brucella canis• Brucella ovisPrimary Antigen• M and A• A and M• A and M• Rough• Rough
  • 3. Blue colonies = Smooth Brucella sp.Crystal colonies = Rough Brucella sp.
  • 4. Safeguarding Animal HealthImmune response to Brucella infection• Cell mediated response• Humoral or antibody production
  • 5. Safeguarding Animal HealthType: JPGWebsite for this image... and faster response - antibody titre is much higher, occurs much faster, ...classes.midlandstech.edu•Full-size image•760 × 600 (1.2x larger), 66KB•More sizes•Search by image•Similar imagesImages may be subject to copyright.
  • 6. Safeguarding Animal HealthSerologic Assays Antibody detection Serum Milk Rapid Inexpensive Sensitivity and Specificity Assays can be impacted by Vaccination Environmental conditionsBrucella cardtest
  • 7. Brucellosis Diagnostic Tests
  • 8. Brucellosis Diagnostic Tests
  • 9. Safeguarding Animal HealthBrucellosis Diagnostic Tests
  • 10. Brucellosis Diagnostic Tests
  • 11. Brucellosis Diagnostic Tests
  • 12. Brucellosis Diagnostic Tests
  • 13. Other Brucella Diagnostic Tests• Polymerase Chain Reaction (PCR)• Culture Identification• Western Blot
  • 14. Amount of Isotype (ng)Test IgM IgG1 IgG2 IgATube 20 ----- 125 650Card ----- 600 7500 -----Riv ----- 1550 2750 -----CF 6500 290 ----- -----ELISA 155 190 220 700
  • 15. Brief History 19th century – Bruce isolated organismfrom human spleens Linked organism to raw goat’s milk Genus named after him 19th century – Bang discoveredorganism that caused abortions incattle Named organism Bacillus abortus 20th century – Evans recognized therelationship between Bruce’s organismand Bang’s organism Advocated pasteurization of milk Re-named organism Brucella abortusSir DavidBruceL.F. BernhardBangAlice C. Evans
  • 16. Acknowledge Superior LaboratorySupport• De Anne Wall, Diane Davis, BarbKesl, Molly Fritz– Serology Laboratory TechniciansQuestions?