Overview of research in the USDA Infectious Bacterial Diseases Unit
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Overview of research in the USDA Infectious Bacterial Diseases Unit

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Presented by Steven Olsen at a Workshop: An Integrated Approach to Controlling Brucellosis in Africa, Addis Ababa, 29-31 January 2013

Presented by Steven Olsen at a Workshop: An Integrated Approach to Controlling Brucellosis in Africa, Addis Ababa, 29-31 January 2013

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    Overview of research in the USDA Infectious Bacterial Diseases Unit Overview of research in the USDA Infectious Bacterial Diseases Unit Presentation Transcript

    • Overview of ResearchIn the USDA Infectious Bacterial Diseases UnitWorkshop: An Integrated Approach to Controlling Brucellosisin Africa, Addis Ababa, 29-31 January 2013Dr. Steven OlsenDr. Steven OlsenNational Animal Disease CenterNational Animal Disease CenterAmes, IA 50010Ames, IA 50010
    • USDA Animal Health Facilities andUSDA Animal Health Facilities andExpertiseExpertiseState-of-the-art:State-of-the-art:•ABSL2 and ABSL3 smallABSL2 and ABSL3 smalland large animal facilitiesand large animal facilities•BSL3 Agriculture largeBSL3 Agriculture largeanimal facilityanimal facilityAnimal care and facilitiesAnimal care and facilitiesoperations expertise thatoperations expertise thatsupports high-containmentsupports high-containmentresearch in all majorresearch in all majorlivestock and severallivestock and severalwildlife specieswildlife species
    • abortusabortus) in the United) in the UnitedStatesStates0200040006000800010000120001982 1985 1988 1991 1994 1997 199919912011
    • Lipopolysaccharide structureLipopolysaccharide structureof virulent and vaccine strainsof virulent and vaccine strainsLipid ABrucella abortus strain 19Brucella abortus field strainsBrucella abortus strain RB51Sugars O-side chainKDOLipid A KDO Sugars O-side chainLipid A SugarsKDOThe O-side chain is the immunodominant antigenof Brucella for antibody responses
    • Efficacy of RB51 as aEfficacy of RB51 as aCalfhood Vaccine for CattleCalfhood Vaccine for CattleAge atVaccinationProtection from abortionRB51 Strain 19 Control% protected (# aborted/ #challenged)10 months 100% (0/20) 100% (0/6) 45% (6/11)7 months 100% (0/22) 100% (0/5) 63% (4/11)5-6 months 92% (2/25) 100% (0/4) 57% (6/14)3 months 87% (2/15) 100% (0/4) 50% (5/10)Overall 95% (4/82) 100% (0/19) 54% (21/46)
    • Distribution of Feral Swine in theDistribution of Feral Swine in theUSUSGPS mapping at http://128.192.20.53/infsms/
    • Brucellosis in the GreaterBrucellosis in the GreaterYellowstone Area (GYA)Yellowstone Area (GYA)
    • = =Variance in ImmunologicVariance in ImmunologicResponses between RuminantsResponses between Ruminants==
    • “Lysine decarboxylase”Ab GAAGATGTGA GGAGTAGGGC AGTAGGGC AGT::::: :::::::: ::::::: ::::AAAGAMel GAAGATGTGA GGAGTAGGGC AGT::::: :::::::: :::::::: ::::::: ::::AAAGASuis GAAGATGTGA GGAGTAGGGC AGTAGGGC AGTAGGGC AGTAGGGC AGTAGGGC AGTAAAGA
    • Alleles(numberofrepeatunitsperlocus)12345678910111213So.CaliforniaSo.FloridaCent.FloridaAlabamKansasArkansasElk-WYOklahomaElk-MTBison-MTCent.FloridaCent.Florida14 1717 2121 1616 1919VNTR-1VNTR-1 VNTR-2VNTR-2 VNTR-3VNTR-3 VNTR-4VNTR-4 VNTR-5VNTR-5 VNTR-6VNTR-6 VNTR-7VNTR-7 VNTR-8VNTR-8 VNTR-9VNTR-9 VNTR-10VNTR-10