Evaluation of stress resistant Sweetpotato  Varieties and their Low cost Micro-  propagation: Ethiopian Experience    Eman...
Introduction
Objectives
Materials and Methods1. Sweet potato varietal screening against   drought and sweetpotato weevil• Twenty one varieties in ...
Materials and Methods• The varieties tested were:• Southern Ethiopia (PIPI, Kudade, Kero, Koka12, Koka6,  Boreda, Damota, ...
Participatory evaluation
Participatory evaluation
Materials and Methods2. Low Cost Micropropagation of Sweetpotato• Four varieties of sweetpotato namely Awassa-83  and Bele...
Materials and Methods
Materials and Methods
Micro-propgation
Micro-propagation
Results and Discussion
Table 1. Performance of sweetpotato varieties                         evaluated at HumboVarieties       Sc (%)     FE (1-5...
Table 2. Performance of sweetpotato varieties                           evaluated at BabileVarieties        Sc (%)        ...
Tab 3. Height, number off shoots /node, fresh and dry weight of shoots of 5 weeks   old Beletech variety grown on differen...
Table 4.Height, number of shoots per node, fresh and dry weight of shoots of 5 weeks oldAwassa- 83 variety grown on differ...
Cost analysis of the substituted low cost medium             Table 5. Comparative costs of culture medium componentsCompon...
Fig. 1. Comparative costs of culture medium          with various substituted components.                                 ...
Conclusion and                  RecommendationConclusion                             Recommendation•   6 varieties in sout...
Acknowledgment• We are very much grateful to Bio-Innovate for  funding and Addis Ababa University for  hosting the project
Thank you very much! Thank you for your Attention!
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Evaluation of stress resistant sweetpotato varieties and their low cost micro-propagation: Ethiopian experience

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Presented by Emana Getu, Tileye Feyissa and Addisu Nega (Addis Ababa University, College of Natural Sciences) at the First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013


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Evaluation of stress resistant sweetpotato varieties and their low cost micro-propagation: Ethiopian experience

  1. 1. Evaluation of stress resistant Sweetpotato Varieties and their Low cost Micro- propagation: Ethiopian Experience Emana Getu, Tileye Feyissa and Addisu Nega Addis Ababa University, College of Natural SciencesFirst Bio-Innovate Regional Scientific Conference United Nations Conference Centre (UNCC-ECA) Addis Ababa, Ethiopia, 25-27 February 2013
  2. 2. Introduction
  3. 3. Objectives
  4. 4. Materials and Methods1. Sweet potato varietal screening against drought and sweetpotato weevil• Twenty one varieties in southern Ethiopia and nineteen varieties in eastern Ethiopia were screened against drought and sweetpotato weevil under field conditions (at two sites in each region ) for two seasons.
  5. 5. Materials and Methods• The varieties tested were:• Southern Ethiopia (PIPI, Kudade, Kero, Koka12, Koka6, Boreda, Damota, Resisto, Eujumula, Temesgen, Dubo, Ukerewe, Mayai, Orodollo, Beletch, Belela, Kulfo, Awassa83, Guntute and Tulla)• Eastern Ethiopia (Berkume, TIS 8250-7, Cuba-1, CN-1753-17, Korojo-2, Korojo, Bekale-A, Bukariso, Bekale-B, TIS-9465-2, TIS9465-2, TIS-9068-8, TIS-8250-1, Awassa-83, TIS-70357-5, CN-1752-9, TIS-9065-1, TIS8441-3 and TIS82/0602-11)• Design used: RCBD with Replications• Plot size: 6m X 2m• Data collected: Stand count, Dry weight, Weevil density, tubers infested by weevils, yield, etc.
  6. 6. Participatory evaluation
  7. 7. Participatory evaluation
  8. 8. Materials and Methods2. Low Cost Micropropagation of Sweetpotato• Four varieties of sweetpotato namely Awassa-83 and Beletech from HARC, and Adu and Barkumie from Haramaya University were collected and used as a stock source for micropropagation.• For low cost micro-propagation study, so far Beletech and Awassa-83 were used
  9. 9. Materials and Methods
  10. 10. Materials and Methods
  11. 11. Micro-propgation
  12. 12. Micro-propagation
  13. 13. Results and Discussion
  14. 14. Table 1. Performance of sweetpotato varieties evaluated at HumboVarieties Sc (%) FE (1-5 scale) EE (1-5 scale) % tubers infested Yield (t/ha) by SPwAwassa-83 50±2.3a 1 1 5.00±0.20a 6.43±0.12aBelela 50±2.3a 3 3 45.00±2.10b 4.17±0.01bBeletch 65±3.2a 1 1 8.00±0.31a 6.35±0.12aKulfo 25±0.8b 1 1 3.00±0.11a 7.640.23aResisto 20±0.6b 1 1 6.50±0.28a 8.61±0.33aTemesgen 65±3.2a 1 1 4.80±0.20a 7.35±0.34aTulla 40±1.8ab 1 1 9.70±0.4a 6.25±0.12aOrdolo 0±0c - - - -Falha 5±0.03c - - - -
  15. 15. Table 2. Performance of sweetpotato varieties evaluated at BabileVarieties Sc (%) FE (1-5 scale) EE (1-5 scale) Yield (t/ha)Berkume 100.00±7a 2 2 12.44±0.8aTis-8250-7 100.00±7a 5 3 2.22±0.2dCuba-1 100.00±7a 3 5 7.97±0.5bCN-1753-17 98.33±6.5a 5 3 4.47±0.3cKorojo-2 98.33±6.5a 3 5 5.33±0.4cCN-1753-14 95.00±5.8a 3 3 1.33±0.1dKorojo 93.33±5.3a 5 5 1.11±0.1dBekale-A 95.00±5.8a 5 5 2.48±0.2dBukariso 98.33±6a 4 3 5.92±0.4cBekale-b 95.00±5.8a 4 3 2.56±0.2dTis-9465-2 98.33±a6 2 2 9.56±0.7aTis-9068-8 65.00±32b 4 3 3.81±0.2dTis-8250-1 93.04±5.7a 1 2 10.74±0.7aAwassa-83 95.00±5.8a 3 2 5.22±0.4cTis-70357-5 100.00±7a 2 3 10.44±0.7aCN-1752-9 100.00±7a 1 1 12.14±0.8aTis-9065-1 100.00±7a 3 2 7.33±0.5bTis-8441-3 88.21±5.6ab 5 5 2.44±0.2dTis-82/0602-11 98.33±6b 5 4 3.22±0.2d
  16. 16. Tab 3. Height, number off shoots /node, fresh and dry weight of shoots of 5 weeks old Beletech variety grown on different low cost media, values given as mean ± SEMedia Height (cm) No Shoot/Node FW (g) DW(g)M1 6.6±0.258a 2.90±0.475a 1.52±0.097a 0.1373±0.010aM2 6.7±0.341ab 2.97±0.195ab 1.44±0.108ab 0.1230±0.008abM3 5.9±0.254abc 2.63±0.206abc 1.28±0.100abc 0.1109±0.009bcM4 6.5±0.309acd 2.96±0.200abcd 1.36±0.137abcd 0.1248±0.012abcdM5 5.7±0.343bcde 2.50±0.178abcde 1.32±0.086abcde 0.1120±0.007bcdeM6 5.7±0.257bcdef 2.27±0.209abcdef 1.18±0.064abcdef 0.1055±0.005bcdefM7 5.8±0.280bcdef 2.67±0.175abcdef 1.42±0.084abcdef 0.1270±0.007bcdefMeans followed by the same letter (s) within a column are not significantly dfferent fromeach other at 5% (LSD) .
  17. 17. Table 4.Height, number of shoots per node, fresh and dry weight of shoots of 5 weeks oldAwassa- 83 variety grown on different low cost medium, values given as mean ± SE.Media Height (cm) No Shoot/Node FW (g) DW(g)M1 7.4±0.362a 2.83±0.424a 1.69±0.140a 0.1480±0.012aM2 6.9±0.406ab 2.73±0.159ab 1.58±0.114ab 0.1393±0.010abM3 5.6±0.392c 2.23±0.164abc 1.45±0.117abc 0.1226±0.009abcM4 5.8±0.326cd 2.90±0.154abcd 1.51±0.119abcd 0.1260±0.009abcdM5 5.8±0.317cde 2.43±0.207abcde 1.40±0.094abcde 0.1161±0.008bcdeM6 5.5±0.338cde 2.43±0.123abcdef 1.19±0.102cde 0.1052±0.008cdefM7 6.5±0.342abcde 2.70±0.174abcdef 1.53±0.104abcde 0.1218±0.008abcdef Means within each column connected by the same superscript (a-f) are not significantly different at 5% probability level
  18. 18. Cost analysis of the substituted low cost medium Table 5. Comparative costs of culture medium componentsComponent of Cost/Kg, L Conc./L (%, w/v) Cost/L (Birr) Cost reductionsthe media (Birr) compared to the standard (%)Agar 2700 0.8 21 0Starch 300 6 18 14.3Cassava flour 10 8 0.80 96.2Lab. Sucrose 640 3 19.20 0Table sugar 14.50 3 0.44 97ddH2O 5 5 0Rain Water 0 0 100Use of rain water instead of ddH2O; the substitution of laboratory grade sucrose with tablesugar; Substitution of gelling agents with cassava flour and starch alone reduced the cost of
  19. 19. Fig. 1. Comparative costs of culture medium with various substituted components. Medium Totally substituted medium M7 (rain water instead of ddH2O; table sugar as carbonsource and starch as a gelling agent) reduced the total cost of the medium by 59.2% and totally substituted medium M6 (rain water instead of ddH2O; table sugar as carbon
  20. 20. Conclusion and RecommendationConclusion Recommendation• 6 varieties in southern Ethiopia • The best varieties should and 4 varieties from eastern go through national Ethiopia were found to be resistant to stresses Variety release system and• For all the parameter be released measured, the plantlets • Agronomic performance obtained on all low cost media of sweetpotato derived showed a good growth from low cost micro- performance. propagation should be• The low cost media was especially good for the number tested. of shoots/ node.
  21. 21. Acknowledgment• We are very much grateful to Bio-Innovate for funding and Addis Ababa University for hosting the project
  22. 22. Thank you very much! Thank you for your Attention!
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