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Biopesticide seed treatments for improved growth of maize seedlings

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Presented by Daniel M. Kang`ethe at the First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013 …

Presented by Daniel M. Kang`ethe at the First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013

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  • 1. Biopesticide seed treatments forimproved growth of maize seedlings Daniel M. Kang`ethe Research and Development Team, The Real IPM Company, (Kenya) Ltd First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013 © The Real IPM Company Thika, Kenya
  • 2. summary- Objective- Background- Methodology/procedure- Criteria of evaluating growth promotion.- Assessment- Conclusion © The Real IPM Company Thika, Kenya
  • 3. Back groundConsortium of:Jomo Kenyatta University of Agriculture andTechnology (Kenya) (JKUAT)Alpha Seeds, Tanzania.The Real IPM Company (K) Ltd, Kenya © The Real IPM Company Thika, Kenya
  • 4. Real IPMBiocontrol company based in Thika, KenyaDelivery of Biopesticide on the seed.Many advantages of seed delivery © The Real IPM Company Thika, Kenya
  • 5. Research ObjectiveTo assess the growth promoting effect of Biopesticides (T. asperellum, B. subtilis, M. anisopiliae and T. asperellum + M. anisopiliae) as seed treatment on maize. © The Real IPM Company Thika, Kenya
  • 6. ProcedureOrigin of Biopesticides Manufactured by Real IPM Kenya. Formulated specially for seed coating. Maize was used as test plant.Design for on farm application © The Real IPM Company Thika, Kenya
  • 7. Procedure cont… © The Real IPM Company Thika, Kenya
  • 8. Procedure cont.. © The Real IPM Company Thika, Kenya
  • 9. Procedure cont…Above plate pictures of (a) Seed coated maize sample and the controland (b) colony growth © The Real IPM Company Thika, Kenya
  • 10. Treatments1. T. asperellum2. M. anisopiliae3. B. subtilis4. T. asperellum + M. anisopiliae5. ControlGermination andseedling growth assessments © The Real IPM Company Thika, Kenya
  • 11. Parameters• Germination• Tap root length• Number of feeder roots• Shoot height• Fresh weight of the seedlings © The Real IPM Company Thika, Kenya
  • 12. Results - Germination 100 90 83.07 83.33 79.6 77.33 80GERMINATION % 70 54 60 50 40 30 20 10 0 BACILLUS CONTROL METARHIZIUM TRICHODERMA TRICHODERMA + METARHIZIUM TREATMENTS © The Real IPM Company Thika, Kenya
  • 13. Shoot length 10 8.92 9 8.31 7.34 8SHOOT LENGTH (cm) 7.27 7 6 4.72 5 4 3 2 1 0 BACILLUS CONTROL MET + METARH TRICHO TRICHO TREATMENTS © The Real IPM Company Thika, Kenya
  • 14. Tap root length 25 21.53TAP ROOT LENGTH (cm) 20.53 19.21 20 15.59 15 12.01 10 5 0 BACILLUS CONTROL MET + METARH TRICHO TRICHO © The Real IPM Company Thika, Kenya
  • 15. Number of feeder roots 7 5.93 5.6 6 5.4NUMBER OF FEEDER ROOTS 5.27 5 4.4 4 3 2 1 0 BACILLUS CONTROL MET + TRICHO METARH TRICHO TREATMENTS © The Real IPM Company Thika, Kenya
  • 16. © The Real IPM Company Thika, Kenya
  • 17. AssessmentHow many microbes on the seed?Biopesticides cfu`s/ Maize seedTrichoderma asperellum 9.2 X10 7Bacillus subtilis 7.5 X10 6Metarhizium anisopliae 1.3 X10 6 © The Real IPM Company Thika, Kenya
  • 18. Conclusion Biopesticides enhance growth Act as growth promoters Widely reported effect Complex mechanism Other pest and disease benefits (See JKUAT paper) © The Real IPM Company Thika, Kenya
  • 19. Conclusion What next Cropping trials have begun Seed company application (as opposed to on farm treatment) Looking for seed company partners in the region Please contact Real IPM/me © The Real IPM Company Thika, Kenya
  • 20. Contacts:info@realipm.comwww.realipm.com Thank you © The Real IPM Company Thika, Kenya