Biopesticide seed treatments forimproved growth of maize seedlings                       Daniel M. Kang`ethe Research and ...
summary- Objective- Background- Methodology/procedure- Criteria of evaluating growth promotion.- Assessment- Conclusion   ...
Back groundConsortium of:Jomo Kenyatta University of Agriculture andTechnology (Kenya) (JKUAT)Alpha Seeds, Tanzania.The Re...
Real IPMBiocontrol company based in Thika, KenyaDelivery of Biopesticide on the seed.Many advantages of seed delivery     ...
Research ObjectiveTo assess the growth promoting effect of  Biopesticides (T. asperellum, B. subtilis, M.  anisopiliae and...
ProcedureOrigin of Biopesticides  Manufactured by Real IPM Kenya.  Formulated specially for seed coating.  Maize was used ...
Procedure cont…                  © The Real IPM Company                    Thika, Kenya
Procedure cont..                   © The Real IPM Company                     Thika, Kenya
Procedure cont…Above plate pictures of (a) Seed coated maize sample and the controland (b) colony growth                  ...
Treatments1.   T. asperellum2.   M. anisopiliae3.   B. subtilis4.   T. asperellum + M. anisopiliae5.   ControlGermination ...
Parameters•   Germination•   Tap root length•   Number of feeder roots•   Shoot height•   Fresh weight of the seedlings   ...
Results - Germination                100                 90                           83.07                               ...
Shoot length                    10                                                             8.92                     9 ...
Tap root length                       25                                                  21.53TAP ROOT LENGTH (cm)       ...
Number of feeder roots                         7                                                                    5.93  ...
© The Real IPM Company  Thika, Kenya
AssessmentHow many microbes on the seed?Biopesticides            cfu`s/ Maize seedTrichoderma asperellum   9.2 X10   7Baci...
Conclusion   Biopesticides enhance growth   Act as growth promoters   Widely reported effect   Complex mechanism   Other p...
Conclusion   What next   Cropping trials have begun   Seed company application   (as opposed to on farm treatment)   Looki...
Contacts:info@realipm.comwww.realipm.com   Thank you               © The Real IPM Company                 Thika, Kenya
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Biopesticide seed treatments for improved growth of maize seedlings

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Presented by Daniel M. Kang`ethe at the First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013

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Biopesticide seed treatments for improved growth of maize seedlings

  1. 1. Biopesticide seed treatments forimproved growth of maize seedlings Daniel M. Kang`ethe Research and Development Team, The Real IPM Company, (Kenya) Ltd First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013 © The Real IPM Company Thika, Kenya
  2. 2. summary- Objective- Background- Methodology/procedure- Criteria of evaluating growth promotion.- Assessment- Conclusion © The Real IPM Company Thika, Kenya
  3. 3. Back groundConsortium of:Jomo Kenyatta University of Agriculture andTechnology (Kenya) (JKUAT)Alpha Seeds, Tanzania.The Real IPM Company (K) Ltd, Kenya © The Real IPM Company Thika, Kenya
  4. 4. Real IPMBiocontrol company based in Thika, KenyaDelivery of Biopesticide on the seed.Many advantages of seed delivery © The Real IPM Company Thika, Kenya
  5. 5. Research ObjectiveTo assess the growth promoting effect of Biopesticides (T. asperellum, B. subtilis, M. anisopiliae and T. asperellum + M. anisopiliae) as seed treatment on maize. © The Real IPM Company Thika, Kenya
  6. 6. ProcedureOrigin of Biopesticides Manufactured by Real IPM Kenya. Formulated specially for seed coating. Maize was used as test plant.Design for on farm application © The Real IPM Company Thika, Kenya
  7. 7. Procedure cont… © The Real IPM Company Thika, Kenya
  8. 8. Procedure cont.. © The Real IPM Company Thika, Kenya
  9. 9. Procedure cont…Above plate pictures of (a) Seed coated maize sample and the controland (b) colony growth © The Real IPM Company Thika, Kenya
  10. 10. Treatments1. T. asperellum2. M. anisopiliae3. B. subtilis4. T. asperellum + M. anisopiliae5. ControlGermination andseedling growth assessments © The Real IPM Company Thika, Kenya
  11. 11. Parameters• Germination• Tap root length• Number of feeder roots• Shoot height• Fresh weight of the seedlings © The Real IPM Company Thika, Kenya
  12. 12. Results - Germination 100 90 83.07 83.33 79.6 77.33 80GERMINATION % 70 54 60 50 40 30 20 10 0 BACILLUS CONTROL METARHIZIUM TRICHODERMA TRICHODERMA + METARHIZIUM TREATMENTS © The Real IPM Company Thika, Kenya
  13. 13. Shoot length 10 8.92 9 8.31 7.34 8SHOOT LENGTH (cm) 7.27 7 6 4.72 5 4 3 2 1 0 BACILLUS CONTROL MET + METARH TRICHO TRICHO TREATMENTS © The Real IPM Company Thika, Kenya
  14. 14. Tap root length 25 21.53TAP ROOT LENGTH (cm) 20.53 19.21 20 15.59 15 12.01 10 5 0 BACILLUS CONTROL MET + METARH TRICHO TRICHO © The Real IPM Company Thika, Kenya
  15. 15. Number of feeder roots 7 5.93 5.6 6 5.4NUMBER OF FEEDER ROOTS 5.27 5 4.4 4 3 2 1 0 BACILLUS CONTROL MET + TRICHO METARH TRICHO TREATMENTS © The Real IPM Company Thika, Kenya
  16. 16. © The Real IPM Company Thika, Kenya
  17. 17. AssessmentHow many microbes on the seed?Biopesticides cfu`s/ Maize seedTrichoderma asperellum 9.2 X10 7Bacillus subtilis 7.5 X10 6Metarhizium anisopliae 1.3 X10 6 © The Real IPM Company Thika, Kenya
  18. 18. Conclusion Biopesticides enhance growth Act as growth promoters Widely reported effect Complex mechanism Other pest and disease benefits (See JKUAT paper) © The Real IPM Company Thika, Kenya
  19. 19. Conclusion What next Cropping trials have begun Seed company application (as opposed to on farm treatment) Looking for seed company partners in the region Please contact Real IPM/me © The Real IPM Company Thika, Kenya
  20. 20. Contacts:info@realipm.comwww.realipm.com Thank you © The Real IPM Company Thika, Kenya
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