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A total ten strains of Pseudomonas spp. were isolated frompaddy soil. Among isolated strains three Pseudomonasisolates P1, P2 and P3were shown siderophore production on succinic acid medium and chromo azural S agarplate medium.The ability of Pseudomonas to grow and to produce siderophores is dependent on the iron content and the type of carbon sources in the medium. Four basal media, supplemented with different concentration of iron, were employed to study the effectof iron and different organic carbon sources on siderophore production in Pseudomonas isolates.Cell growth reached a maximal value with150µ/ml Fe3+ siderophore production was maximum at this iron concentration. The optimal iron concentration for high siderophore production was in the succinate medium.The cultures under study, growth of cultures increasing with the increased concentration of iron up to 60µM, where as siderophore production repressed at high concentration of iron. Maximum siderophore production was 94, 88, 83 units for P1, P2 and P3 isolates respectively. All three isolates have shown both type of siderophore production i.e. wine red color formation in supernatant indicated production of hydroxamate type (pyoverdine) while yellow color formation in supernatant showed presence of catecholate or phenolate type (pyochelin) siderophore.