Int. J. Pharm. Res. Sci., 2014, 02(1), 98-103.
www.ijprsonline.com
ISSN: 2348 –0882
======================================...
Int. J. Pharm. Res. Sci., 2014, 02(1), 98-103.
www.ijprsonline.com
ISSN: 2348 –0882
======================================...
Int. J. Pharm. Res. Sci., 2014, 02(1), 98-103.
www.ijprsonline.com
ISSN: 2348 –0882
======================================...
Int. J. Pharm. Res. Sci., 2014, 02(1), 98-103.
www.ijprsonline.com
ISSN: 2348 –0882
======================================...
Int. J. Pharm. Res. Sci., 2014, 02(1), 98-103.
www.ijprsonline.com
ISSN: 2348 –0882
======================================...
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Biosynthesis Of Silver Nanoparticles Using Curcuma Longa And Their Antibacterial Activity

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A rapid advance of nanotechnology has the potential approach for significant improvements in disease prevention, diagnosis and treatment. In this article, we report a simple and eco-friendly biosynthesis of silver nanoparticles (Ag-NPs) using silver nitrate as metal precursor in Curcuma longa. These Ag-NPs were characterized by UV–vis spectroscopy, and Transmission electron microscopy (TEM). These nanoparticles exhibited maximum absorbance in specific nano meter range in UV–vis spectroscopy. TEM micrographs revealed the formation of well-dispersed Ag-NPs with its size and morphology. Microbiology assay founds that Ag-NPs are effective against V.cholera bacteria. These developments raise exciting opportunities to diagnose and treat pathogenic mode of infection based on the various profiles to target diseases.

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Biosynthesis Of Silver Nanoparticles Using Curcuma Longa And Their Antibacterial Activity

  1. 1. Int. J. Pharm. Res. Sci., 2014, 02(1), 98-103. www.ijprsonline.com ISSN: 2348 –0882 ============================================================================== Biosynthesis Of Silver Nanoparticles Using Curcuma Longa And Their Antibacterial Activity S Elumalai1 and R Devika2 1 Associate professor, Dept. of Plant Biology & Plant Biotechnology, Presidency College, Chennai, India. 2 Research scholar, Dept. of Plant Biology & Plant Biotechnology, Presidency College, Chennai, India. Email: devinanotech25@gmail.com Abstract A rapid advance of nanotechnology has the potential approach for significant improvements in disease prevention, diagnosis and treatment. In this article, we report a simple and eco-friendly biosynthesis of silver nanoparticles (Ag-NPs) using silver nitrate as metal precursor in Curcuma longa. These Ag-NPs were characterized by UV–vis spectroscopy, and Transmission electron microscopy (TEM). These nanoparticles exhibited maximum absorbance in specific nano meter range in UV–vis spectroscopy. TEM micrographs revealed the formation of well-dispersed Ag-NPs with its size and morphology. Microbiology assay founds that Ag-NPs are effective against V.cholera bacteria. These developments raise exciting opportunities to diagnose and treat pathogenic mode of infection based on the various profiles to target diseases. Keywords: Silver nanoparticles, C. longa , Spectroscopic studies, Antibacterial Activity 1. Introduction A novel biosynthesis of nanoparticles is an exciting methods that have attracted significant attention due to their potential use in many applications, such as catalysis, drug delivery biosensor [1] antimicrobials and therapeutics [2,3]. New application of nanoparticles and nanomaterials are emerging rapidly [4]. Biological methods of nanoparticles synthesis using microorganism, enzyme, andplant or plant extract have been suggested aspossible ecofriendly alternatives to chemical andphysical methods [5]. As part of our work, we have observed that aqueous silver ions, when exposed to the rhizome extract of C. longa, are reduced in solution, thereby leading to the formation of an extremely stable silver particle. 2. Materials and Methods 2.1 Collection and Extract Preparation The rhizomes of C. longa were rinsed with fresh seawater and distilled water to remove associated debris. The cleaned material was then air dried to dryness in the shade at 30°C. The dried samples were finely powdered and stored at -20°C until use. Approximately 10 g of C. longa biomass was taken in a conical flask containing 100 mL of distilled water, kept for 24 hrs and then the aqueous solution components were separated by filtration. To this solution, AgNO3 (10-3 M) was added and kept for several hours at 24 hrs Periodically, aliquots of the reaction solution were removed and the absorptions were measured in a Elico UV-Vis spectrophotometer. 2.2 Synthesis and Characterization For the synthesis of Ag- NPs 1ml of rhizome extracts as test solution were incubated at room temperature for 1-2 hours. The silver nanoparticle solution thus obtained was purified by repeated 98
  2. 2. Int. J. Pharm. Res. Sci., 2014, 02(1), 98-103. www.ijprsonline.com ISSN: 2348 –0882 ============================================================================== centrifugation at 15,000 rpm for 20min. Supernatant is discarded and the pellet is dissolved in deionised water. It is well known that Ag-NPs exhibit yellowish brown color in aqueous solution due to excitation of surface plasmon vibrations in Ag-NPs [6]. The silver nanoparticles were confirmed by colour changes and qualitatively characterized by UV-visible spectrophotometer on a Elico UV- Vis spectrophotometer. The bioreduction of Ag+ ions in solution was monitored using UV-Vis spectroscopyfrom zerotime reading was noted and double distilled water as blank, and incubated at culture condition. The samples were withdrawn at various time intervals and the absorbance was measured [7]. 2.3 UV-Vis spectroscopy analysis UV-Vis Spectral analysis was done by using HITACHI U-2900 Spetrophotometer. The UV-Vis Spectrophotometer analysis reveals the formation of silver nanoparticles by showing surface Plasmon resonance at 422 nm. UV-Vis Spectroscopy is one of the most widely used techniques for structural characterization of silver nanoparticles. The absorption spectrum of the brown silver colloids prepared by hydrazine reduction showed a surface Plasmon absorption band with a maximum of 422 nm indicating the presence of spherical or roughly spherical silver nanoparticles [8]. 2.4 TEManalysis of silver nanoparticles Sample for TEM analysis was prepared as mentioned in IR sample preparations. The sample was first sonicated (Vibronics VS 80) for 5 min. Silver nanoparticles was loaded on carbon-coated copper grids and solvent was allowed to evaporate under Infra light for 30 min. TEM measurements were performed on Phillips model CM 20 instrument operated at an accelerating voltage at 200 Kv. 2.5 Antibacterial activity: Bactericidal effects of Ag-NPs were studied against Vibrio cholera bacteria. Antimicrobial activity was demonstrated by modified method described [9]. Then 0.1 ml of the diluted microbial cultures was spread on sterile nutrient agar plate. The soaked and dried discs of 6 mm diameter of Whatman filter paper No: 1 were then placed on the seeded plates and gently pressed down to ensure contact [10]. Four replicates were placed for control, Ag-NPs, antibiotics and Ag-NPs combined with antibiotics Chloramphenicol, in each disc to confirm the inhibition a zone, and the plates were incubated at 37°C for 24 hours. After incubation period, the inhibition zone around the discs were measured and recorded, as the difference in diameter between the disc (6 mm) and growth free zone of V. cholera were measured. 2.6 Results & Discussion Silver nanoparticles were synthesized from AgNO3 solution containing Ag+ ions by treating with the rhizome extracts. The color of the solution changed to deep brownish color within 30 min of reaction with the Ag+ ions. The appearance of the deep brownish color indicated formation of silver nanoparticles. The formation of silver nanoparticles was confirmed by color changes followed by UVVis spectrophotometer analysis. It is generally recognized that UV-Vis spectroscopy could be used to examine size and shape-controlled nanoparticles in aqueous suspensions [11]. The UV-Vis spectrophotometer proved to be very useful technique for the analysis of some metal 99
  3. 3. Int. J. Pharm. Res. Sci., 2014, 02(1), 98-103. www.ijprsonline.com ISSN: 2348 –0882 ============================================================================== nanoparticles. The UV- visible spectra (shown in Fig 1) indicated a strong Plasmon resonance that was located at ~422 nm. Presence of this strong broad plasmon peak had been well documented for various Me- NPs, with sizes ranging all the way from 2 to 100 nm. The microstructures and size of the biosynthesized silver nanoparticles were studied by TEM analysis. The typical TEM images of the silver nanoparticles synthesized by rhizome extract as reducing agent are shown in Fig. 2. The micrograph shows formation of spherical like morphology. The spherical like particles show very small size about 5-10 nm. 100
  4. 4. Int. J. Pharm. Res. Sci., 2014, 02(1), 98-103. www.ijprsonline.com ISSN: 2348 –0882 ============================================================================== 2. Rai, M., Yadav, A., Gade, A., 2009. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27, 76-83. 3. Elechiguerra, J,L., Morones, J,R., Burt, J.L., Camacho, A,B., Gao, X., 2005. The bactericidal effect of silver nanoparticles with HIV-1. J Nanobiotechnology 3, 6. 4. Naiwa, H.S., Hand Book of Nanostructural Materials and Nanotechnology 2000.11, 1– 5p. Conclusion The silver nanoparticles synthesized using extracts of rhizome samples was confirmed by color changes and was characterized by UV-visible spectrophotometer; the UV-visible spectra showed a broad peak located at 422nm for silver nanoparticles. The TEM analysis shows large spherical shape particle with 200 nm size and a small spherical like 5-10 nm size particles. This technique has proved to be very useful for the synthesis of nanoparticles from biological material. Hence, we conclude that the synthesized nanoparticles from C. longa more efficient due to its biological origin and its smaller size and it can be further analyzed for the usage in drug delivery process and anti microbicidal properties. 4. References 1. Jianrong, C., Yuqing, M., Xiaohua, W., Sijjiao, Nanotechnology and BiotechnolAdv 22,505. Nongyue, H., L., 2004. biosensors. 5. Song, J.Y., and Kim, B.S.,2008. Rapid biologicalsynthesis of silver nanoparticles using plant leafextracts. Bioprocess Biosyst Eng. 6,313 6. Shankar, S,S., Ahmad, A., Sastry, M., 2003.Geranium leaf assisted biosynthesis of silver nanoparticles. Biotechnol Prog 19, 1627-1631. 7. Mariekie, G. and Anthony, P.,2006. Microbial production of Gold nanoparticles, Gold Bulletin, 39/1. 8. Mukherjee,P., Senapati,S., Mandal,D., Ahmad,A., Khan,M,I., Kumar,R.,Sastry,M.,2002.Extracellular biosynthesis of bimetallic Au-Ag alloy nanoparticles.Chem. Biochem, 3, 461-463 9. Langfield, R,D., Scarano, F,J., Heitzman, M,E., Kondo, M., Hammond, G,B., 2004. Use of a modified microplate bioassay 101
  5. 5. Int. J. Pharm. Res. Sci., 2014, 02(1), 98-103. www.ijprsonline.com ISSN: 2348 –0882 ============================================================================== method to investigate antibacterial activity in the Peruvian medicinal plant Peperomia galioides. J Ethnopharmacol 94, 279-281. 10. Wiley, B,J., Xiong, Y., Li, Z,Y., Yin, Y., Xia, Y., 2006. Right bipyramids of silver: a new shape derived from single twinned seeds. Nano Lett 6,765-768. 11. Yamanaka, M. and Hara, K., 2005. A review on the application of inorganic nanostructured materials in the modification of textiles. J Appl Environ Microbiol 71,75897593. 102

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