Mycopesticide development for vegetables

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Screening indigenous mycopesticides for DBM control.Field efficacy of Bba5653,Impact of Bba5653 on cabbage yield,Cost-effective production of Bba spores.

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Mycopesticide development for vegetables

  1. 1. Mycopesticide development for vegetables Ignace Godonou and Braima James Presentation at 2009 R4D week, IITA, Ibadan---------------------------------------------------Problems Vegetables are prone to severe pest attack Prevalence of inappropriate pesticide regimes Health hazard and environment pollutionThe need Increase farmer access to biological pesticidesOur response Develop, field-test and promote mycopesticides
  2. 2. Research tasks Identify and collect diseased pest specimens Isolate and culture entomopathogens Screen candidate pathogens for virulence Damaged Select potential pathogens candidate cabbage Develop formulations & delivery systems Demonstrate field efficacy of product Assess safety and eco-toxicology of products Register product for commercialization
  3. 3. Screening indigenous mycopesticides for DBM control Virulence trials  Indigenous (Benin) strains of entomopathogenic fungi against DBM; Source: IITA microbial collections 40 Bba virulence test  Hosts = other Lepidoptera: Acigona %Cummulative dead larvae with external growth of 35 sp, Sesamia calamistis, Eldana 30 Bba5653 saccharina Bba5644 25 Ma182 Bba5645 fungus Bba5654 20  Beauveria bassiana (6): Bba14, Bba14 Bba5655 15 Ma178 Bba5644, Bba5653, Bba5654, SDW Untreated 10 Bba5655 (PDA plated, SDW + 5 Tween80 harvested and applied) 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14  Metarhizium anisopliae (2): Ma178, Days after treatment Ma182 (SDA plated, SDW + Tween80 Bba5653 is most virulent strain; harvested and applied) has good potential against DBM  Inoculum concentration @ 108 conidia/ml; 3rd instar DBM/leaf
  4. 4. Field efficacy of Bba5653 Spores produced in dry powder form 16 1g of powder contains 1011 spores 14 Bba FPR trials on DBM 12 Nombre of live DBM larvae/plant Solitaire + Bba 5653 1g powder in 1l TW + Tween80 10 Tween80 + Bba 5653 (TWT)/or in 1l TW + Solitaire (TWS) as 8 Untreated emulsifier 6 Talstar 4 HV spray of TWT/TWS formulations: 2 0- 750ml/7.2 sq m bed = 1,041l per ha 2 5 8 11 14 17 20 23 26 29 32 35 38 41 Days after application- Dose/bed =7.5 x1010 (=1015 spores/ha)Application frequency of Bba5653 was 50% < bifentrin (= Talstar)
  5. 5. Impact of Bba5653 on cabbage yield DBM population- DBM better controlled in Bba plots- Pesticide resistance to Talstar? 60- Poor quality Talstar? Water-based formulation of Bba5653 Emulsion-base formulation of 50 Bba5653 Cabbage yield Untreated Talstar 40- 44t/ha under Bba5653 Cabbage yield (t/ha)- 15t/ha under Talstar 30- 3x more yield with Bba 20 Product promotion 10- Experiential learning by farmers 0- Awareness/news media coverage Treatment- IITA MoU with NGO-Songhai for regional promotion- IITA MoU with FAES-Dakar for commercial production
  6. 6. Cost-effective production of Bba sporesLocally available substrates Rice as solid substrates used in Bba 5653 production Alternatives to rice as solid substrates needed Screened cassava starch (CS) & oil palm kernel cake (OPKC) 80% CS mixed with 20% OPKC is most suitable alternative to rice 1.6E+09 1.4E+09 Number of conidia/g of substrate 1.2E+09 1.0E+09 80%CS+20%OPKC 75%CS+ 25%OPKC 66.67%CS+33.33%OPKC 8.0E+08 57%CS+43%OPKC 50%CS + 50%OPKC 6.0E+08 100%CS 4.0E+08 2.0E+08 0.0E+00 Treatments
  7. 7. To move this forward… Bba5653 (with a few other Bba isolates) is a Green Muscle spin-offscientific breakthrough that holds great promise in vegetable production There is a strong need to reduce disconnects between such publiclyfunded breakthroughs & their delivery to poor small holder farmers Private sector interest may be dampened by low level profit Explore R4D partnership models with specialized NGOs in which:- IITA increases range of biopesticides; assures sustainable supply of theproducts in the short to medium term; conducts markets studies toquantify demand & profitability; links with ARIs for product safety & eco-toxicological research required to develop registration dossiers- NGOs advocate policy support for regional biopesticide regulatoryframework; market & return % of sales to IITA for further biopesticideresearch; develop capacity to produce biopesticides under IITAsupervision; assure sustainable access of farmers to the products Thank you

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