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Fostering Food Safety Monitoring in SSA

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Problems in containing aflatoxins,Various alfatoxin quantification systems,ELISA: Ideal test for Sub-Saharan Africa,ELISA and data analysis,Quantification of aflatoxins in different commodities

Problems in containing aflatoxins,Various alfatoxin quantification systems,ELISA: Ideal test for Sub-Saharan Africa,ELISA and data analysis,Quantification of aflatoxins in different commodities

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  • 1. Fostering Food Safety Monitoring in SSA P Lava Kumar and R Bandyopadhyay Reduced ability to Malnutrition cope with diseases, Quality reduction especially HIV/AIDS Aflatoxin Liver cirrhosis, immuno-suppression, contamination blocks nutrient absorption, growth Trade in food and feed abnormalities, etc. Restrictions Synergistic interaction Liver cancer with Hepatitis-B & C www.iita.org
  • 2. Problems in containing aflatoxins• The most effective measures to control aflatoxins depends on a program that “monitors” their presence in food and feeds. But, • Monitoring programs in SSA relies on importation of diagnostic tools, which are expensive, some have short shelf-life and prohibitive for majority of the stakeholders.• “Sustainability” depends on the -Sustainable access to basic reagents at affordable costs. -Suitability for application in a minimally equipped labs. www.iita.org
  • 3. Various alfatoxin quantification systems Analytical Methods TLC HPLC LC-MSSerological Methods Immuno-columns ELISA Lateral Flow Strips www.iita.org
  • 4. ELISA: Ideal test for SSA• ELISA have been proven to be easy for adoption in developingcountries. Afla-ELISATM is a simple and low-cost test for quantitative estimation of aflatoxins developed at IITA.• IITA has developed cheap and high-through put screening systems which dramatically reduces the phenotyping cost. www.iita.org
  • 5. Afla-ELISA kitComponents-AFB1-BSA standards -Coating buffer concentrate Coast:-AFB1 standards -Dilution buffer concentrate US$ 2 per sample-AFB1 polyclonal antibodies -Substrate buffer concentrate-Sample preparation & ELISA protocol -Wash buffer concentrate www.iita.org
  • 6. Three simple steps1. Sample Extraction2. ELISA3. Data input into software www.iita.org
  • 7. Sample extraction 3. Mix the suspension (30 min) 4. Filter the extract1. Ground 20 g sample (maize) 2. Resuspend in 100 ml of 0.5% KCL in 70% Methanol 5. Use it in ELISA www.iita.org
  • 8. ELISA and data analysis Final Reaction Measure in an ELISA plate reader set with 405 nm3.000 y = -2.1638x + 2.28692.000 2 R = 0.99531.000 Input data into XL program sheet0.000 0.000 0.500 1.000 1.500 2.000 to estimate AFB1 concentration in-1.000 unknown samples-2.000 www.iita.org
  • 9. Requirements for aflatoxin lab Orbital Shaker ELISA Plate readerBlender Pipette pH Meter ELISA Plates General labware and a computer www.iita.org
  • 10. Quantification of aflatoxins in different commodities millet 1.800 sorghum 1.600 ground nut 1.400 cassava 1.200 riceA405nm 1.000 maize 0.800 Buffer 0.600 0.400 0.200 0.000 1 2 3 4 5 6 7 8 9 Log AfB1(ng/ml) www.iita.org
  • 11. Specificity of Afla-ELISA 120.0 100.0 80.0 B1% binding B2 G1 60.0 G2 FB1 40.0 OTA 20.0 0.0 1 2 3 4 5 LogAfB1(ng/ml) www.iita.org
  • 12. Trade Mark Registration TM Afla-ELISA Next step: Product registration www.iita.org
  • 13. Next Steps and Exit Strategy 1. Research  2. Product Development [OUTPUT]  ExitIITABy By 3rd party 3. Product Deployment [OUTPUT] Fostering utilization by NARS, researchers, monitoring agencies etc) -Product registration - Accreditation - policy -Training and facilitating infrastructure development Definite Exit 3. Utilization of Afla-ELISA for food safety monitoring [OUTCOME] www.iita.org
  • 14. Thank youA&H Program R4D Week 2009 www.iita.org