Occurrences of Wheat Stripe Rust and
Races of Puccinia striiformis f. sp. tritici in
China during 2008-2012
Wanquan CHEN
I...
Contents
 Occurrences of wheat stripe rust
 Survey of Pst races
 Temperature sensitivity of Pst population
 Comprehens...
I Occurrence and Epidemics of Wheat Stripe
Rust in China 2008-2012
China is the largest wheat
producer in the world with ...
Occurrence and Epidemics of Wheat Stripe
Rust in China during1990-2012
Occurrence and Epidemics of Wheat Stripe
Rust in China during 2008-2012
Severe Epidemics of Wheat Stripe Rust in
China in 2009
Severe epidemic areas
GansuGansu
Yunnan
Sichuan
II Survey of Pst Race and Virulence
19 Chinese Differentials
1 Trigo Eureka (Yr6)
2 Fulhard (+)
3 Lutescens 128(+)
4 Menta...
Some of Activities of Field Survey
Distribution of trap nurseries
29 Locations
16 Provinces
> 130 Lines
29 Locations
16 Provinces
> 130 Lines
Trap nurs...
Years Chuanmai
42 (Yr26)
Lantian
17(Yr26)
Guinong
22 (Yr26)
92R137
(Yr26)
Moro
(Yr10)
Mianmai
37 (Yr+)
Zhou
98167
(Yr+)
20...
Dynamics of Major Pst Races in China During
2008-2012
A total of 295 virulence
phenotypes has been detected
from more than...
Distribution of Major Pst Race Groups in China
During 2008-2012
III Temperature Sensitivity of Different Spatio-
Temporal Pst Populations in China
† Temperature sensitivities of 126 Pst ...
III Temperature Sensitivity of Different Spatio-
Temporal Pst Populations in China
• The ET50s of all tested isolates were...
III Temperature Sensitivity of Different Spatio-
Temporal Pst Populations in China
Low temperature-sensitivity isolates (h...
III Temperature Sensitivity of Different Spatio-
Temporal Pst Populations in China
Two hours after heat shock at 26°C, mRN...
IV Comprehensive Utilizations of Pst Races in Wheat
Breeding for Rust Resistance
1. Screening and evaluating for rust
resi...
IV Comprehensive Utilizations of Pst Races in Wheat
Breeding for Rust Resistance
2. Supplying Race Information & Uredinios...
IV Comprehensive Utilizations of Pst Races in Wheat
Breeding for Rust Resistance
3. Developing Wheat Lines for Stripe Rust...
IV Comprehensive Utilizations of Pst Races in Wheat
Breeding for Rust Resistance
3. Developing Wheat Lines for Stripe Rust...
IV Comprehensive Utilizations of Pst Races in Wheat
Breeding for Rust Resistance
4. Reasonable Deployment of Wheat Cultiva...
Acknowledgments
Research Group of Wheat Fungal Diseases in IPPCAAS
Shichang XU Xiayu DUAN Yilin ZHOU
Taiguo LIU Li GAO Rui...
Acknowledgments
National Wheat Rust Collaborative Group
Sponsor: MOA
Coordinator: IPPCAAS
Collaborators: 4 Universities
9 ...
Acknowledgments
Supporting Projects and Grants
 Public Service (Agriculture) Research Project (200903035)
 National Foun...
THANK YOU!
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2nd iwsrs turkey 2014

  1. 1. Occurrences of Wheat Stripe Rust and Races of Puccinia striiformis f. sp. tritici in China during 2008-2012 Wanquan CHEN IPPCAAS
  2. 2. Contents  Occurrences of wheat stripe rust  Survey of Pst races  Temperature sensitivity of Pst population  Comprehensive utilization of Pst races  Acknowledgments
  3. 3. I Occurrence and Epidemics of Wheat Stripe Rust in China 2008-2012 China is the largest wheat producer in the world with the total wheat growing area of 24 million ha and the total wheat production of over 120 billion kg annually (MOA, 2012) Wheat stripe rust is destructive in all winter wheat regions and is considered the most important foliar disease in China
  4. 4. Occurrence and Epidemics of Wheat Stripe Rust in China during1990-2012
  5. 5. Occurrence and Epidemics of Wheat Stripe Rust in China during 2008-2012
  6. 6. Severe Epidemics of Wheat Stripe Rust in China in 2009 Severe epidemic areas GansuGansu Yunnan Sichuan
  7. 7. II Survey of Pst Race and Virulence 19 Chinese Differentials 1 Trigo Eureka (Yr6) 2 Fulhard (+) 3 Lutescens 128(+) 4 Mentana(+) 5 Virgilio (YrVir1-2) 6 Abbondanza(+) 7 Early Piemium(+) 8 Funo (YrA+) 9 Danish 1 (Yr3) 10 Jubilejina II(YrJu1-4) 11 Fengchan3 (Yr1) 12 Lovrin13 (Yr9+) 13 Kangyin655(Yr1,YrKy1-2) 14 Suwon11 (YrSu) 15 Zhong4(+) 16 Lovrin10(Yr9) 17 Hybrid46(Yr3b,4b) 18 T. spelta album (Yr5) 19 Guinong22 (Yr10, 24/26?) 19 Chinese Differentials 1 Trigo Eureka (Yr6) 2 Fulhard (+) 3 Lutescens 128(+) 4 Mentana(+) 5 Virgilio (YrVir1-2) 6 Abbondanza(+) 7 Early Piemium(+) 8 Funo (YrA+) 9 Danish 1 (Yr3) 10 Jubilejina II(YrJu1-4) 11 Fengchan3 (Yr1) 12 Lovrin13 (Yr9+) 13 Kangyin655(Yr1,YrKy1-2) 14 Suwon11 (YrSu) 15 Zhong4(+) 16 Lovrin10(Yr9) 17 Hybrid46(Yr3b,4b) 18 T. spelta album (Yr5) 19 Guinong22 (Yr10, 24/26?) † Regular field surveys was conducted annually across wheat growing areas in China. † Trap nurseries were disposed at 29 locations in 16 provinces. † Stripe rust race identification was conducted by the Chinese National Wheat Rust Collaborative Group (CNWRCG).
  8. 8. Some of Activities of Field Survey
  9. 9. Distribution of trap nurseries 29 Locations 16 Provinces > 130 Lines 29 Locations 16 Provinces > 130 Lines Trap nurseries Provincial capitals
  10. 10. Years Chuanmai 42 (Yr26) Lantian 17(Yr26) Guinong 22 (Yr26) 92R137 (Yr26) Moro (Yr10) Mianmai 37 (Yr+) Zhou 98167 (Yr+) 2009 0 0 0 0 0 0 0 2010 1/10/10 0 0 0 0 0 0 2011 2-3/10/20 3/10/t 3/5/5 3/10/10 2/10/20 0 0 2012 3/20/100 3/10/60 3/10/50 3/40/80 3/10/100 0 0 2013 3/10/60 3/10/40 2/10/20 3/20/60 3/10/30 3/10/40 3/10/10 Resistance Variation of Some Wheat Lines in Trap Nursery during 2009-2013 (Tianshui, Gansu Province)
  11. 11. Dynamics of Major Pst Races in China During 2008-2012 A total of 295 virulence phenotypes has been detected from more than 4770 samples during 2008-2012. CYR32 & CYR33 is considered as dominant races with the frequency of 21.7% (18.15- 25.22% ) and 22.1% (18.27- 25.87%) V26 (Virulence to Yr24/26) was firstly detected with the frequency of 4.3% in 2010, and then increased to 13.2% in 2012 V26
  12. 12. Distribution of Major Pst Race Groups in China During 2008-2012
  13. 13. III Temperature Sensitivity of Different Spatio- Temporal Pst Populations in China † Temperature sensitivities of 126 Pst isolates sampled from 12 provinces collected over four decades (1980s-2010s) were compared. † ET50 values representing the temperature required to achieve a 50% DIR (disease inhibition rate), was taken as the temperature sensitivity parameter of the pathogen. Origin HB YN GZ SC CQ XZ HN GS QH XJ SX NX Number of samples 20 9 11 15 6 7 7 16 10 6 12 7
  14. 14. III Temperature Sensitivity of Different Spatio- Temporal Pst Populations in China • The ET50s of all tested isolates were abnormally distributed in a range from 18.46 to 27.01°C, and 14.29% of isolates were higher than 26°C • Pst isolates from warmer, lower elevations/latitudes showed relatively higher ET50 values (revealing LT).
  15. 15. III Temperature Sensitivity of Different Spatio- Temporal Pst Populations in China Low temperature-sensitivity isolates (high ET50 value) had much stronger parasitic fitness than the mid- and high temperature sensitivity isolates, as displayed by shorter latent periods, more spore production and faster lesion growth. HT isolates LT isolates 15 days after inoculation under 26°C
  16. 16. III Temperature Sensitivity of Different Spatio- Temporal Pst Populations in China Two hours after heat shock at 26°C, mRNA expression of hsp70 was 17.4-fold higher in LT isolate than in the control (untreated urediniospores) and 4.9-fold higher than HT isolate, suggesting that the hsp70 gene may play an important role in Pst adaptation to high temperature .
  17. 17. IV Comprehensive Utilizations of Pst Races in Wheat Breeding for Rust Resistance 1. Screening and evaluating for rust resistance in wheat improvement program IPP is responsible for the disease resistance evaluation of NATWV 1. Screening and evaluating for rust resistance in wheat improvement program IPP is responsible for the disease resistance evaluation of NATWV Years No IM MR-HR Slow Rusting % 2009 137 22 37 22 51.1 2010 187 43 29 2 39.6 2011 183 43 56 - 54.1 2012 133 23 50 26 74.4 2013 148 17 50 38 70.9 Total 788 148 222 88 58.1
  18. 18. IV Comprehensive Utilizations of Pst Races in Wheat Breeding for Rust Resistance 2. Supplying Race Information & Urediniospores2. Supplying Race Information & Urediniospores Results of Pst race survey is distributed to MOA, NATESC, provincial stations, and institutions of wheat breeding etc Urediniospores of various Pst races is supplied for the institutions of wheat breeding annually
  19. 19. IV Comprehensive Utilizations of Pst Races in Wheat Breeding for Rust Resistance 3. Developing Wheat Lines for Stripe Rust Resistance-NILs3. Developing Wheat Lines for Stripe Rust Resistance-NILs Two sets of near-isogenic lines: - Mingxian169 (winter wheat)---BC6F4: 9 Yr genes: Yr1, Yr2, Yr5, Yr7, Yr9, Yr10, YrSp, YrKy2, YrResubka - Taichung 29 (spring wheat)---BC6F7: 10 Yr genes: Yr1, Yr2, Yr5, Yr7, Yr9 Yr10, YrV23, YrSp, YrKy2, YrJu4
  20. 20. IV Comprehensive Utilizations of Pst Races in Wheat Breeding for Rust Resistance 3. Developing Wheat Lines for Stripe Rust Resistance--- Multilines 3. Developing Wheat Lines for Stripe Rust Resistance--- Multilines ‘Zhongzhi 2’ Shaan 167 (Yr5/Yr10/YrC591) Some Wheat Advanced Lines for Stripe Rust Resistance Resistant Multi-lines
  21. 21. IV Comprehensive Utilizations of Pst Races in Wheat Breeding for Rust Resistance 4. Reasonable Deployment of Wheat Cultivars based on the Distribution of Pst Races 4. Reasonable Deployment of Wheat Cultivars based on the Distribution of Pst Races Deployment of Yr Genes in Inoculum Source Areas
  22. 22. Acknowledgments Research Group of Wheat Fungal Diseases in IPPCAAS Shichang XU Xiayu DUAN Yilin ZHOU Taiguo LIU Li GAO Ruiming LIN Weihua LI Jing FENG Bo LIU Jieru FAN Fengtao WANG
  23. 23. Acknowledgments National Wheat Rust Collaborative Group Sponsor: MOA Coordinator: IPPCAAS Collaborators: 4 Universities 9 Provincial institutes 3 Provincial extension stations
  24. 24. Acknowledgments Supporting Projects and Grants  Public Service (Agriculture) Research Project (200903035)  National Foundation for Wheat Industry and Technology Expert (CARS-03- 04B)  The National Key Basic Research Program of China (2013CB127700 and 2011CB100403)  ……
  25. 25. THANK YOU!

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