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Lecture 7 Corynebacterium and listeria
 

Lecture 7 Corynebacterium and listeria

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    Lecture 7 Corynebacterium and listeria Lecture 7 Corynebacterium and listeria Presentation Transcript

    • ‫بسم ا الرحمن الرحيم‬ GENUS: CORYNEBACTERIUM Prof. Khalifa Sifaw Ghenghesh
    • • • • • • Diptheroides = Coryneforms Gram +ve, Nonmotile Rods. Arranged in V forms or Palisades Catalase +ve
    • 1. Corynebacterium diphtheriae • Diphtheria. Morphology • Pleomorphic Bacilli • Cells Contain Metachromatic Granules.
    • Corynebacterium diphtheriae Club-shaped, Gram-positive rods
    • Cultural Characteristics • i. Loeffler's Serum Slope. • ii. Tellurite BA. - Temp. 37oC, - Aerobic and Facultative AnO2.
    • Corynebacterium diphtheriae forms black colonies on tellurit agar (left), on blood agar colonies appear white (right)
    • PATHOGENICITY • Diphtheria is a Toxaemia. – Exotoxin: M. wt 62000 – 2 Fragaments >> A & B – Inhibits Protein Synthesis. – Has Special Affinity for Certain Tissues "Mycocardium, Adrenals, Nerve Endings". • Toxin >> Toxoid. • Site of Infection >> Faucial. – Toxin >> Pseudomemebrane.
    • LABORATORY DIAGNOSIS • Important >> Specific Treatment Should Be Started Immediately on Suspicion of Diphtheria. • Specimen: Two Swabs from the Lesion. • Culture: i. Loeffler's Serum Slope >> Albert Stain. ii. Tellurite BA. iii. BA >> Streptococcal or Staphylococcal Pharyngitis.
    • PATHOGENICITY TESTS • In-vivo Tests: i. Subcutaneous Test: ii. Intracutaneuos Test: • In-vitro Test: – Elek Test ( Gel Precipitaion Test). • Tissue Culture:
    • TREATMENT • i. Antitoxin: > Moderate Cases: > Severe Cases: • ii. Antibiotic: PREVENTION • i. Active Immunization >> PDT. • ii. Passive Immunization: • iii. Combined:
    • SCHICK TEST • > Injection of 0.2 ml of Diphtheria Toxin Intradermally. • > Positive Reaction >> Erythema + Swelling
    • SCHICK TEST ______________________________________________________________________ Result Test Arm Control Arm Interpretation Diphthri (Toxin) Inactivated Toxin Immunization 36h 120h 36h 120h ______________________________________________________________________ Negative ----Immune, Not Not Hypersensitive Required --------------------------------------------------------------------------------------Positive + + -- -- Not Immune, Not Required Hypersensitive --------------------------------------------------------------------------------------------------------------------Negative + -+ -Immune Not Hypersensitive Required --------------------------------------------------------------------------------------------------------------------Positive & + + + -Not Immune ContraPseudo Hypersensitive indicated ______________________________________________________________________
    • ‫بسم ا الرحمن الرحيم‬ Listeria monocytogenes
    • • Listerosis. • • • • • G+ve Coccobacilli > in Short Chains. Motile at 25oC But Not at 37oC. Aerobic to Microaerophilic. On BA > Narrow Zone of β-Haemolysis. Listerolysin O >
    • • Incidence and Transmission > DISEASE • i. In Adults: • ii. Neonatal Infections: a. Early Type: b. Late Type:
    • LABORATORY DIAGNOSIS • i. Specimen: Depend on Syndrome: Blood, CSF, Genital Tract Secretions. • ii. Media: Selective Media. TREATMENT • Penicillin, Ampicillin.