Lecture 31 Mycoplasms

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Lecture 31 Mycoplasms

  1. 1. ‫بسم ا الرحمن الرحيم‬ MYCOPLASMAS Prof. Khalifa Sifaw Ghenghesh
  2. 2.  Pleomorphic organisms  No cell wall – Resistant to antibiotics act on bacterial cell wall synthesis and to lysozyme  Require animal protein (blood serum) and sterol for cultivation  On agar – Colonies have “fried egg” appearance  Two Important genera – Mycoplasma – Ureaplasma
  3. 3. Mycoplasma pneumoniae colonies (40X)
  4. 4. Mycoplasma pneumoniae
  5. 5. Mycoplasma & Ureaplasma  Important species associated with human disease –M. fermentans –M. genitalium –M. hominis –M. pneumoniae –Ureaplasma urealyticum
  6. 6. Pathogenesis 1. Respiratory Infections  Primary atypical pneumonia – M. pneumoniae  Respiratory infection in the newborn – M. hominis and U. urealyticum  Other respiratory infection – M. hominis and M. fermentans?
  7. 7. 2. Urogenital Infections Mainly M. hominis and U. urealyticum  Urogenital infections in men >> NGU  Urinary infection and calculi – Acute pylonephritis – Patients with infective stones  Reproductive tract disease in women – Bacterial vaginosis – PID  Disease associated with pregnancy and newborn
  8. 8. 3. Joint Infections U. Urealyticum and M. genitalium  Sexually acquired reactive arthritis  Reiter’s disease and rheumatoid arthritis 4. Infection in the Immunocompromised M. penumoniae, M. hominis and ureaplasms  Severe pneumonia, arthritis, persistent urethritis and chronic cystitis
  9. 9. Laboratory Diagnosis 1. Respiratory Infections  Specific serological test – Complement fixation test  Isolation of M. pneumoniae – Specimen: sputum, throat washing, pharyngeal swab – Specific selective liquid and solid media – Requires up to 3 weeks incubation ?  PCR
  10. 10. 2. Urogenital Infections  Isolation of the causative organism – Specimen: Urethral, cervical or vaginal swabs – Specific selective liquid media – Culture on solid media  Serological tests – For definitive identification  PCR – DNA primers used for amplification by PCR
  11. 11. Treatment 1. Respiratory Infections  M. pneumoniae – Tetracycline, Erythromycin, Clarithromycin, Ciprofloxacin 2. Urogenital Infections – Tetracyclines, Erythromycin plus clindamycin

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