‫بسم ا الرحمن الرحيم‬

GENUS: BRUCELLA
Prof. Khalifa Sifaw Ghenghesh


Aerobic, Gram-negative bacilli







Appear as cocci, coccobacilli and short
bacilli

Non-motile, non-capsulate
O...
 Important

species

B. melitensis >> goats & sheep
 B. abortus
>> cattle
 B. suis
>> pigs

 Important:

All 3 specie...
Brucella species

gram-negative bacilli
Brucella abortus
PATHOGENESIS









Incubation period: 10-30 days
Symptoms may be delayed for months
Enter the body through:
 Ski...
LABORATORY DIAGNOSIS
1. Blood Culture
 Specimen


Blood (10 ml volumes)

 Inoculate





Blood culture tubes or bott...
Brucella melitensis colonies
2. Serological Tests
 Standard

agglutination test
 Mercaptoethanol test
 Complement fixation test
 Coobs’ test

3. Br...
TREATMENT AND CONTROL


Combination of streptomycin and
tetracycline or rifampicin and
doxycycline



Vaccination of you...
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Lecture 25 Brucella

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Lecture 25 Brucella

  1. 1. ‫بسم ا الرحمن الرحيم‬ GENUS: BRUCELLA Prof. Khalifa Sifaw Ghenghesh
  2. 2.  Aerobic, Gram-negative bacilli      Appear as cocci, coccobacilli and short bacilli Non-motile, non-capsulate Optimum temp. >> 37oC Facultative intracellular parasites Essentially pathogens of goats, Cattle, sheep and pigs  Causative agents of Brucellosis (typical zoonosis) worldwide  Also known as Malta, Mediterranean or undulant fever
  3. 3.  Important species  B. melitensis >> goats & sheep  B. abortus >> cattle  B. suis >> pigs  Important: All 3 species can infect man and domestic animals
  4. 4. Brucella species gram-negative bacilli
  5. 5. Brucella abortus
  6. 6. PATHOGENESIS       Incubation period: 10-30 days Symptoms may be delayed for months Enter the body through:  Skin abrasions, mucous membranes of the alimentary or respiratory tracts and sometimes the conjunctivae>> reach the blood through regional lymphatics. Symptoms may vary without the fluctuating temperature to act as guide. Bacteria localize in various parts of the reticulo-endothelial system resulting in complications in any part of the body. Symptoms include: malaise, low-grade fever, lassitude, insomnia, irritability and swelling around the joints.
  7. 7. LABORATORY DIAGNOSIS 1. Blood Culture  Specimen  Blood (10 ml volumes)  Inoculate    Blood culture tubes or bottles (glucose-serum broth) Incubate in 10% CO2 Cultures should be retained for at least 6-8 weeks before being discarded as negative
  8. 8. Brucella melitensis colonies
  9. 9. 2. Serological Tests  Standard agglutination test  Mercaptoethanol test  Complement fixation test  Coobs’ test 3. Brucellin Skin Test
  10. 10. TREATMENT AND CONTROL  Combination of streptomycin and tetracycline or rifampicin and doxycycline  Vaccination of young cattle (6-8 months) Pasteurization of milk or milk products Eradication by compulsory testing animals and slaughtering positive reactors.  

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