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Lecture 25 Brucella
 

Lecture 25 Brucella

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    Lecture 25 Brucella Lecture 25 Brucella Presentation Transcript

    • ‫بسم ا الرحمن الرحيم‬ GENUS: BRUCELLA Prof. Khalifa Sifaw Ghenghesh
    •  Aerobic, Gram-negative bacilli      Appear as cocci, coccobacilli and short bacilli Non-motile, non-capsulate Optimum temp. >> 37oC Facultative intracellular parasites Essentially pathogens of goats, Cattle, sheep and pigs  Causative agents of Brucellosis (typical zoonosis) worldwide  Also known as Malta, Mediterranean or undulant fever
    •  Important species  B. melitensis >> goats & sheep  B. abortus >> cattle  B. suis >> pigs  Important: All 3 species can infect man and domestic animals
    • Brucella species gram-negative bacilli
    • Brucella abortus
    • PATHOGENESIS       Incubation period: 10-30 days Symptoms may be delayed for months Enter the body through:  Skin abrasions, mucous membranes of the alimentary or respiratory tracts and sometimes the conjunctivae>> reach the blood through regional lymphatics. Symptoms may vary without the fluctuating temperature to act as guide. Bacteria localize in various parts of the reticulo-endothelial system resulting in complications in any part of the body. Symptoms include: malaise, low-grade fever, lassitude, insomnia, irritability and swelling around the joints.
    • LABORATORY DIAGNOSIS 1. Blood Culture  Specimen  Blood (10 ml volumes)  Inoculate    Blood culture tubes or bottles (glucose-serum broth) Incubate in 10% CO2 Cultures should be retained for at least 6-8 weeks before being discarded as negative
    • Brucella melitensis colonies
    • 2. Serological Tests  Standard agglutination test  Mercaptoethanol test  Complement fixation test  Coobs’ test 3. Brucellin Skin Test
    • TREATMENT AND CONTROL  Combination of streptomycin and tetracycline or rifampicin and doxycycline  Vaccination of young cattle (6-8 months) Pasteurization of milk or milk products Eradication by compulsory testing animals and slaughtering positive reactors.  