Aeromonas from drinking water in Tripoli-Libya

455 views
269 views

Published on

Published in: Technology, Business
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total views
455
On SlideShare
0
From Embeds
0
Number of Embeds
9
Actions
Shares
0
Downloads
20
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Aeromonas from drinking water in Tripoli-Libya

  1. 1. ISOALTION, HEMOLYTIC ACTIVITY AND ANTIBIOTIC SUSCEPTIBILITY OF AEROMONAS SPECIES FROM DRINKING WATER OBTAINED FROM WATER RESERVOIRS (FASGHIA) IN TRIPOLI-LIBYA Zakia M. El-Agili, Halima Nashnoush, Khalifa Sifaw Ghenghesh*, and Amal Rahouma* Dept. of Botany1, Faculty of Science, and *Dept. of Medical Microbiology2, Faculty of Medicine, Tripoli University, Tripoli-Libya
  2. 2. INTRODUCTION Aeromonas Gram -ve, oxidase +ve bacilli. Habitat Natural waters Disease bacteremia, wound infections and gastroenteritis. Virulence factors hemolysins, Cytotoxins Adherence – Mannose-resistant hemagglutination
  3. 3. Treatment Resistant to β-lactams Chromosomally mediated Cephalosporins, fluoroquinolones Aims Fasghia is an underground reservoir to collect rain water used for drinking. To isolate Aeromonas species from drinking water obtained from fasghias in Tripoli. To determine the virulence factors of the isolated Aeromonas. To determine the susceptibility of the isolated Aeromonas to antibiotics.
  4. 4. MATERIAL AND METHODS Samples From October 2003-July 2004, 56 samples obtained from fasghias in Tripoli were examined Microbiology Samples were enriched in alkaline peptone water (APW, pH 8.6) at 37oC. After overnight incubation, a loopful from APW was plated on ampicillin blood agar (ABA, 15mg/L). Plates were incubated at 37oC. After overnight incubation, suspected colonies were tested for oxidase and identified using standard microbiological procedures and API20E Isolated Aeromonas were tested for hemolysin production on BA, agglutination of RBCs in absence and presence of mannose, and susceptibility to antibiotics by the disc diffusion method.
  5. 5. RESULTS Of 56 water samples examined, 41 (73%) were positive for Aeromonas species. Speciation of Aeromonas 10 (24.4%) A. hydrophila, 10 (24.4%), A. caviae, 9 (22%) A. sobria, and 12 (29.2%) atypical aeromonads. Hemolysin production was detected in all (100%) Aeromonas isolates.
  6. 6. Of the 41 Aeromonas isolates 20 (48.8%) showed mannose-sensitive hemagglutination. 14 (34.1%) showed mannose-resistant hemagglutination.
  7. 7. Resistance of Aeromonas from drinking water obtained from fasghias in Tripoli-Libya Antibiotic No (%) resistant (n=41) ---------------------------------------------------------------------Ampicillin 31 (75.6) Amoxicillin + 14 (34.1) clavulanic acid Amikacin 3 (7.3) Chloramphenicol 15 (36.6) Ciprofloxacin 1 (2.4) Nalidixic acid 3 (7.3%) Tetracycline 1 (2.4%) Trimethoprim2 (4.8%) sulphamethoxazole
  8. 8. CONCLUSION Antibiotic-resistant pathogenic Aeromonas species are common in drinking water from water reservoirs (Fasghia) in Tripoli.

×