8. AB SOLiD PI System
Ion Torrent Proton
AB SOLiD 4hq System
Complete Genomics
454 GS 20
Illumina Genome Analyzer
PacBio RS
AB SOLiD 4 System
Illumina Genome Analyzer II
454 GS FLX
AB SOLiD 3 Plus System
AB SOLiD System 2.0
454 GS FLX Titanium
Illumina HiSeq 2000
Ion Torrent PGM
Illumina Genome Analyzer IIx
AB SOLiD System 3.0
AB 5500 Genetic Analyzer
Illumina HiScanSQ
454 GS FLX+
AB 5500xl Genetic Analyzer
454 GS
Helicos HeliScope
Illumina HiSeq 1000
AB SOLiD System
Illumina MiSeq
Illumina HiSeq 2500
454 GS Junior
シーケンサー別 約2ヶ月間のRun数増加率
70.0%
60.0%
50.0%
40.0%
30.0%
20.0%
10.0%
0.0%
30. Whole Genome Amplification
from 100ng FFPE
• Use of GenomiPhi V2 (GE Healthcare)
– SNP and small indel calling
– Not ideal for breakpoint/fusion gene
detection
• Use of GenomePlex WGA2 (Sigma)
– breakpoint analysis possible at the
expense of some loss of read length
because of required adapter trimming
• Avoid if possible
– amplification of reference DNA can impact
somatic mutation calling (LOH calls)
– amplification of tumor DNA may generate
false positives