Sheba M.J. MohanKumar, Priya Balasubramanian, Katryn
Allen, James G. Wagner, Jack R. Harkema and
Neuroendocrine Research Laboratory and Laboratory
of Inhalation Pathology, College of Veterinary
Medicine, Michigan State University, E. Lansing, MI,
Ozone and Particulate matter
Ozone- “good” and “bad”
Bad ozone-generated at
ground level by automotive
Smog – auto exhaust also
contains air particles.
Biological effects: irritates
lungs, upper respiratory tract,
increases allergic airway
Air pollution - relevance to One
Air pollution also
Increased risk for
Obesity and Air pollution
Obesity is widespread. Increased risk for
cardiovascular and metabolic disorders
Obesity is also linked to stress
Asthma is more common in obese patients
Would exposure to Ozone and CAPs aggravate
respiratory and cardiovascular conditions in obese
Adult male Sprague Dawley rats
Normal chow or high fructose diet for 6 weeks
Expose to Air or Ozone
Brains removed and sectioned
Isolation of Paraventricular hypothalamic nucleus for
Median eminence for CRH-ELISA
Serum for corticosterone-RIA
HF diet by itself increases norepinephrine and
corticosterone- suggesting stress axis activation.
Animals on normal diet react more to CAPs
Animals on HF diet react to CAPs and Ozone
combination. Obesity may increase susceptibility to
these dual exposures.
Elevations in Norepinephrine levels can also increase
risk for cardiovascular conditions.
Controlling CAPs and ozone pollution is important
Assessment: Do CAPs and ozone increase risk for
allergic airway disease? Other biological effects,
Manage: Reduce automotive vehicle use- reduce
gasoline and solvent use. Electric vehicles, walk/bike.
Use cleaner energy sources. Susceptible populations
should stay indoors during high ozone periods.
Communicate: Increase awareness.