In this presentation, GE Life Sciences scientists take you through the basics of tagged protein purification with tips and tricks to guide you to successful purification; covering affinity tags, expression systems and planning both expression and purification of a protein. How to deal with some of the most cited problems in tagged protein purification; including protein degradation, low yield, purity, tag removal and protein stability.
Affinity Chromatography - principles
Tags & Expression systems -strategies
Tips & Hints
Tag cleavage & removal