LentiVectors, EpiVectors, TransVectors
Differences between the three cycles

First let’s see how a classic Lentiviral
vect...
LentiVectors

After fusion with the cell membrane, the
vector genome enters the cytoplasm.

© 2014 GEG-Tech, all rights re...
LentiVectors

The RNA vector genome is then transcribed
by the viral reverse-transcriptase (RT) into
linear DNA.
© 2014 GE...
LentiVectors

After translocation in the cell nucleus, the
DNA is integrated into the cell chromatin by
the HIV-1 integras...
LentiVectors

Using the cell machinery, your protein of
interest is synthetized from the integrated
vector genome
© 2014 G...
LentiVectors

With the integration of the vector genome,
the protein is assured to have a high-level
and long-term express...
Now, let’s see how a non-integrating
lentiviral vector works

© 2014 GEG-Tech, all rights reserved
EpiVectors

Beside integrating into the chromatin, the
DNA of viral origin can also form circles…

© 2014 GEG-Tech, all ri...
EpiVectors

… which can be used as template for
transcription.

© 2014 GEG-Tech, all rights reserved
If we disable the viral integrase…

© 2014 GEG-Tech, all rights reserved
EpiVectors

The vector is no longer integrated but only
circularized…

© 2014 GEG-Tech, all rights reserved
EpiVectors

… those circles are now the only template
used for your protein synthesis.

© 2014 GEG-Tech, all rights reserv...
EpiVectors

Due to the dilution effect of cell growth,
these circles can provide short-term
expression in dividing cells.
...
EpiVectors

However in non-dividing cells, you will have a
long-term expression, yet lower than from
integrated vectors.
©...
If you want transient expression of your
transgene even in non-dividing cells,
TransVectors are what you need.
How does it...
TransVectors

In a classic lentiviral vector, the reversetranscriptase binds to a specific region of the
vector genome cal...
TransVectors

In our TransVectors, the PBS region is deleted
from the vector genome.

© 2014 GEG-Tech, all rights reserved
TransVectors

The binding of RT and reverse-transcription
are made impossible.
© 2014 GEG-Tech, all rights reserved
TransVectors

And without reverse-transcription, the whole
cycle is impaired.

© 2014 GEG-Tech, all rights reserved
TransVectors

Your protein synthesis now rely on a very
discreet process: translation directly from the
primary RNA vector...
TransVectors

Since RNAs have a short lifetime in the
cytoplasm, the protein expression will be
highly transient, even in ...
LentiVectors, EpiVectors, TransVectors
Differences between the three cycles

The three lines of GEG-tech vectors provide
y...
Viral genome
final state

LentiVectors

EpiVectors

TransVectors

Transgene
expression level

Integrated DNA

Transgene ex...
Upcoming SlideShare
Loading in...5
×

Three types of Lentiviral Vectors - by GEG-tech.com

302

Published on

Published in: Health & Medicine, Technology
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
302
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
0
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Transcript of "Three types of Lentiviral Vectors - by GEG-tech.com"

  1. 1. LentiVectors, EpiVectors, TransVectors Differences between the three cycles First let’s see how a classic Lentiviral vector works. © 2014 GEG-Tech, all rights reserved
  2. 2. LentiVectors After fusion with the cell membrane, the vector genome enters the cytoplasm. © 2014 GEG-Tech, all rights reserved
  3. 3. LentiVectors The RNA vector genome is then transcribed by the viral reverse-transcriptase (RT) into linear DNA. © 2014 GEG-Tech, all rights reserved
  4. 4. LentiVectors After translocation in the cell nucleus, the DNA is integrated into the cell chromatin by the HIV-1 integrase © 2014 GEG-Tech, all rights reserved
  5. 5. LentiVectors Using the cell machinery, your protein of interest is synthetized from the integrated vector genome © 2014 GEG-Tech, all rights reserved
  6. 6. LentiVectors With the integration of the vector genome, the protein is assured to have a high-level and long-term expression © 2014 GEG-Tech, all rights reserved
  7. 7. Now, let’s see how a non-integrating lentiviral vector works © 2014 GEG-Tech, all rights reserved
  8. 8. EpiVectors Beside integrating into the chromatin, the DNA of viral origin can also form circles… © 2014 GEG-Tech, all rights reserved
  9. 9. EpiVectors … which can be used as template for transcription. © 2014 GEG-Tech, all rights reserved
  10. 10. If we disable the viral integrase… © 2014 GEG-Tech, all rights reserved
  11. 11. EpiVectors The vector is no longer integrated but only circularized… © 2014 GEG-Tech, all rights reserved
  12. 12. EpiVectors … those circles are now the only template used for your protein synthesis. © 2014 GEG-Tech, all rights reserved
  13. 13. EpiVectors Due to the dilution effect of cell growth, these circles can provide short-term expression in dividing cells. © 2014 GEG-Tech, all rights reserved
  14. 14. EpiVectors However in non-dividing cells, you will have a long-term expression, yet lower than from integrated vectors. © 2014 GEG-Tech, all rights reserved
  15. 15. If you want transient expression of your transgene even in non-dividing cells, TransVectors are what you need. How does it work ? © 2014 GEG-Tech, all rights reserved
  16. 16. TransVectors In a classic lentiviral vector, the reversetranscriptase binds to a specific region of the vector genome called PBS © 2014 GEG-Tech, all rights reserved
  17. 17. TransVectors In our TransVectors, the PBS region is deleted from the vector genome. © 2014 GEG-Tech, all rights reserved
  18. 18. TransVectors The binding of RT and reverse-transcription are made impossible. © 2014 GEG-Tech, all rights reserved
  19. 19. TransVectors And without reverse-transcription, the whole cycle is impaired. © 2014 GEG-Tech, all rights reserved
  20. 20. TransVectors Your protein synthesis now rely on a very discreet process: translation directly from the primary RNA vector genome © 2014 GEG-Tech, all rights reserved
  21. 21. TransVectors Since RNAs have a short lifetime in the cytoplasm, the protein expression will be highly transient, even in non-dividing cells. © 2014 GEG-Tech, all rights reserved
  22. 22. LentiVectors, EpiVectors, TransVectors Differences between the three cycles The three lines of GEG-tech vectors provide you with the opportunity to delimitate your transgene expression in time. © 2014 GEG-Tech, all rights reserved
  23. 23. Viral genome final state LentiVectors EpiVectors TransVectors Transgene expression level Integrated DNA Transgene expression in: Dividing cells Non-dividing cells High Long term Long term Episomal DNA Medium Transient Long term RNA Low Transient Transient © 2014 GEG-Tech, all rights reserved

×