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GRM 2013: Breeding and selection strategies to combine and validate QTLs for WUE and heat tolerance of wheat in China - R Jing

GRM 2013: Breeding and selection strategies to combine and validate QTLs for WUE and heat tolerance of wheat in China - R Jing






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  • Consistent results indicated that pyramiding of large effect alleles associated with final plant height produced significantly shorter or taller plant. The linear correlations were highly significant.
  • Dr. Tariq M Chattha Plant Breeding Institute University of Sydney

GRM 2013: Breeding and selection strategies to combine and validate QTLs for WUE and heat tolerance of wheat in China - R Jing GRM 2013: Breeding and selection strategies to combine and validate QTLs for WUE and heat tolerance of wheat in China - R Jing Presentation Transcript

  • Breeding and selection strategies to combine and validate QTLs for WUE and heat tolerance of wheat in China RL Jing, XM Chen, et al. Chinese Academy of Agricultural Sciences (CAAS) XM Chen, Q Li, et al. Hebei Academy of Agricultural Sciences (HAAS) MR Sun, XR Li, et al. Shanxi Academy of Agricultural Sciences (SAAS) YQ Zhang, ZL Wu, et al. Xinjiang Academy of Agricultural Sciences (XAAS) M Reynolds. CIMMYT R Trethowan. University of Sydney, Australia GCP General Research Meeting 27‒30 September 2013, Lisbon, Portugal Ruilian Jing (G7010.02.01)
  • Objective  Implementing standardized drought and heat phenotyping protocols to physiologically evaluate genetic populations and germplasm resources in China  Using MARS and the outputs of previous QTL studies to improve water-use-efficiency and heat tolerance of wheat in China − Marker assisted recurrent selection − Introgression of known QTLs into local Chinese cultivars
  • 270 historical winter wheat accessions 60 candidate SSR markers in six chromosome regions QTL for plant height under multi-water regimes --- linkage mapping, association mapping and allele pyramiding Wu et al., J. Exp. Bot., 2010, 61: 2923–2937 Zhang et al., Planta, 2011, 234: 891–902 Beneficial allele detection for DT and HT improvement
  • Phenotypic effects of QTL alleles on plant height Zhang et al., Mol Breeding, 2013, 32: 327–338 a: plant height and number of alleles with negative effects on height b: plant height and number of alleles with positive effects on height Number of elite alleles Number of elite alleles Plant height of lines pyramided alleles 9 lines: 70.9 cm (Xgwm11-1BA208, Xwmc349-4BA103 and Xcfd23-4DA202) 4 lines: 112.8 cm (Xwmc349-4BA101 and Xcfd23-4DA205)
  • SWSC and TGW of modern cultivars from different decades under four water regimes SWSC: stem water-soluble carbohydrates ;SWSCF: SWSC at flowering stage; SWSCG: SWSC at grain-filling stage
  • Favorable allele detection and pyramiding of SWSC and TGW in wheat Single SWSC-favorable allele contributing to significantly higher 1000-grain weight Accumulation (A) and frequency distribution (B) of 16 favorable alleles in modern cultivars from different decades
  • Confirmation and selection of QTLs for physiological traits associated with DT and HT  11 markers linked with two QTLs  QTLs for parameters of chlorophyll fluorescence kinetics (Fv/Fm, 9)  Canopy temperature depression (CTD, 12)  Stem water soluble carbohydrate (SWSC, 31)  Chlorophyll content (CC, 29)  Plant height (PH, 5) Using joint linkage-association mapping, 70 validated QTL markers for physiological traits related to drought and heat tolerance were selected as candidate markers for marker assisted recurrent selection (MARS).
  • Six populations selected for MARS Original cross Generation No. of line Polymorphic marker Top line New cross Jinmai 47×shaanyou 225 BC3F5 215 23 10 20F2 Jingdong 8×Aikang 58 F2:4 207 36 8 16F1 Yannong 19×Yunhan 618-2 F6 395 13 9 15F2; 18F1 Hengguan 35×Jifeng 3703 F3:4 320 27 17 46F1 Chang 6878×Chang 4738 F2:4 220 12 13 37F1 Xinchun 6×PASTOR F5 224 19 19 10F2; 30F1  Six populations were selected based on the SNP polymorphism between 25 parent pairs used in the original crosses by KBioscience UK.  Based on both phenotyping and genotyping diversities, 8-19 top lines have been selected from the populations for pyramiding the beneficial alleles.  Total of 192 crosses, including F1 and F2 lines have been developed. Populations developed for MARS
  • Before winter After winter Grain filling stage Phenotyping of GCP reference set WPHYSGP in three Chinese wheat zones Winter Wheat Zone Changzhi, SAAS CK Sowed on 2011-10-20Sowed on 2011-10-5 Facultative Wheat Zone Yuncheng, SAAS Sowed on 2011-10-20 Before winter After winter Before winter Spring Wheat Zone Urumchi, XAAS
  • GCP materials F2 populationBC1F1 population GCP lines F2 population Introgression of genetic variation from GCP lines into Chinese cultivars BC1F1 population 25 elite lines were selected from the GCP reference set WPHYSGP based on the agronomic traits under the artificial protection during winter in winter wheat zones. 29 elite lines with favourable agronomic traits and high grain yield were selected in Urumqi, Xinjiang, Spring Wheat Zone, in well-watered, heat stress, and water stress environments. These elite lines have been used to make more than 200 crosses with the local cultivars to the F2 ~ BC3F3 generations .
  • Advanced line selection  Two elite lines have been submitted to the National Trial.  Seven elite lines have been submitted to the Provincial Trial in Spring Wheat Zone, Facultative Wheat Zone and Winter Wheat Zone, respectively.  A number of advanced lines participant evaluating experiments in breeding programs.
  • Zhongmai 36 enters 2nd year National Trial Xgwm 369 Xwmc 47 Zhongmai 36 Zhongmai 36, an elite line selected from a backcross [(Jinmai 47×Shaanyou 225)×Jinmai 47] BC3F5 has been recommended to enter the 2013-2014 National Trial for the 2nd year in Yellow-Huai River Valley in the rainfed environments. Zhongmai 36 possesses beneficial alleles of Xgwm 369 and Xwmc 47 for SWSC and TGW. From left to right: Jinmai 47, Shaanyou 225, Zhongmai 36
  • Summary Research achievements:  70 QTL markers for DT and HT have been used in MARS.  Six populations were selected and 192 crosses were made with tail top lines.  46 elite WPHYSGP lines were used as cross parents, made >200 crosses in the F2 ~ BC3F3 generations.  Two and 7 elite lines have been submitted to the National and Provincial Trial, respectively.  A group of young scientists are growing up. Plan for next step:  To validate more QTLs for WUE and HT.  To enhance MARS in the breeding program.  To deliver DT and HT lines to breeders for breeding utilization.  To select elite lines based on genotyping and phenotyping for various wheat zones.  To exchange information and technology among breeders and geneticists for building the capacity of wheat molecular breeding.