GRM 2011: Wheat Research Initiative progress report

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GRM 2011: Wheat Research Initiative progress report

  1. 1. GCP Wheat meeting:a summaryHyderabad, September 21-22, 2011
  2. 2. GCP Wheat projectsG3008.01 Generating new wheat germpalsm withenhanced drought/heat tolerance using AB genomegenetic diversityG3008.08 Breeder-friendly high throughput phenotypingtools to select for adaptive traits in drought environmentsG7010.02.01: Wheat breeding and selection strategies tocombine and validate QTLs for WUE and heat tolerancein ChinaG7010.02.02: Wheat breeding and selection strategies tocombine and validate QTLs for WUE and heat tolerancein IndiaG7009.01 Natural variation in the transcritional regulationof drought responses in wheat
  3. 3. G3008.08 Breeder-friendly high throughputphenotyping tools to select for adaptive traitsin drought environmentsAims to identify physiological traits or trait values fordefined drought stresses• phenotyping: ICARDA & CSIRO Australia• 20 – 25 trials of 200 genotypes x 2 years x 72 traits(all characterized with markers, SSRs & DArTs)Opportunity• Phenotyping manual has been developed – the WheatCI also have a manual (circulate & compare)• Learn from this experience in wider Wheat CI project
  4. 4. G3008.01 Generating new wheat germplasm withenhanced drought/heat tolerance using AB genomegenetic diversityMaterials developed:• 1000 DHs (emmer based hexaploids)• 1000’s of F3 – F6 (by end of the project in 2012)• Approx 300 primaries based on 108 emmers x 3 A.tauschiiHow to phenotype close to 3,000 lines?Opportunity• Wheat CI in India can test at key phenotyping sites• ICARDA to test DH lines & primaries• CSIRO (Fernanda Dreccer) DH• PBI Sydney Uni to test DH & primaries
  5. 5. Wheat Challenge Initiative: Molecular breeding and selectionstrategies to combine and validate QTLs for improving WUEand heat tolerance of wheat in India & ChinaIndia:Vinod Prabhu (coordinator) – IARI (Delhi)Virinder Singh Sohu – PAU (Ludhiana)PC Mishra - J.N.K.V.V.(Powarkheda)SC Misra – Agharkar Research Institute, (Pune)NK Singh – NRCPB (Delhi)China:Ruilian Jing (coordinator) – CAAS (Beijing)Xiumin Chen - Hebei Academy of Agricultural Sciences (HAAS)Meirong Sun - Shanxi Academy of Agricultural Sciences (SAAS)Zhenlu Wu - Xinjiang Academy of Agricultural Sciences (XAAS)
  6. 6. Activities1. Evaluation of CIMMYT’s international droughttolerance reference set (200 + lines) at keylocations. Validate known QTLs in each targetenvironment2. MABC - transfer known international QTLs andlocally identified QTLs into elite localgermplasm3. Use MARS to develop elite drought & heattolerant materials
  7. 7. Reference set evaluationIndia:Reference set (from CIMMYT) of 200 linestested at 4 sites, 2 reps and series ofphysiological traits & yield measuredA subset identified for more intensivecharacterization in 2011/12 and for use asdonors for backcrossing
  8. 8. Reference set evaluationChina:The reference set of 145 lines plus localcultivars has been tested 2010/11 at 7 sites.Observation: most sites single row plots,difficulty of planting date (winter or spring),large size of set
  9. 9. PlansDevelop a country subset for 2011/12 + bestadapted lines at each site
  10. 10. Known QTL introgressionIndia:15 sources from the reference set crossed to 4elite Indian backgrounds. Now at BC1F2 (theBC1F1 was sown over summer).A local population C306/WH157 is now in thesecond year of phenotyping
  11. 11. Known QTL introgressionChina11 BC3F4 and BC3F5 populations evaluated inBeijing, Shanxi and Gansu Provinces in 2010/11to validate target QTLs detected in the DHpopulation (Hanxuan 10 × Lumai 14) and othergenetic populations17 Chinese elite cultivars crossed key referenceset materials. A total of 1224 crosses have beenmade. Populations at F2, F3, and BC1F1
  12. 12. Development of MARS materialsIndia:Planning 18 crosses x 2 reps x 4 sites in2011/12Reduce to 6 key crosses (>200progeny/cross) and test in largerplots/more reps to establish best possiblephenotype
  13. 13. Development of MARS materialsChina:Each partner has developed two populations:CAAS – F2:3 (260 lines) & F2Yunchen – F3:5 (600 lines) & F2:3 (500 lines)Hebei – 2 x F2:3 populations (of 300 lines each)Changzhi – 2 x F2:3 populations (of 300 lines each)Xinjiang – F2:4 (1000 lines) & F2:3 (300 lines)Phenotyping in 2011/12: possible reduction to 6 crosses
  14. 14. Germplasm sharing amongpartnersNeed an umbrella document from the GCPdescribing the nature of wheat CI partnership- Listing participant organizations and staff- List germplasm to be shared- Approved by Dr Swapan Datta- Develop project specific MTA that covers allpartners- Chinese position?
  15. 15. Data sharing• Each group to provide a list of data sets in 2 weeks withdata to follow• Data managers have been appointed in each project(India/China/Australia) to manage data quality & uploadinto GCP wheat central• Need to agree on a Wheat CI data dictionary tostandardize trait capture (RT to send the existing Indo-Australia ICIS data dictionary to all partners)• Indo-Australian use case study database needs to bemade public and posted on GCP wheat central atCIMMYT (RT to discuss with partners in February)
  16. 16. Data managers presentationsTariq Chattha – PBI pedigree & phenotypic data loadedsome molecular data- time to produce germplasm lists needs to reduce- need more user friendly molecular data upload- need data query toolsManoj Singh (NRCPB) – highly successful user case: Indo-Australia wheat program- local databases at all nodes and a central data baseset up and populated- data sharing led to conversion of central data basefrom ms Access to MySQL to increase capacity
  17. 17. • Great demonstration of field book and theuse of the Samsung 10.1 Galaxy tablet forrecording field data.• RT to write a letter to ICAR requesting thisfor Wheat CI in India for local purchase
  18. 18. Fingerprinting• SNP validation set (94 lines expanded from 72 initiallyidentified)• 125 Indian lines, 125 Chinese plus 30 globally importantgenotypes (hopefully performing well in India/China) to begenotyped with the same SNPs• RT to facilitate development of the lists with Ruilian & VinodOther genotyping data (pedigrees/markers used)Help with genotype selection for testing/assembly of newsubsets for genotyping• Vinod/Ruilian to coordinate nationally• Indo/Australian ACIAR data available• CIMMYT & ICARDA to provide relevant sets
  19. 19. Ontology• The Wheat group to provide current traitdictionary• Good value seen by all in the new formatOptimas demo• Value of the tool recognized• A need to practice with local data
  20. 20. Capacity building• Level 1 training (basic ICIS use & datamanagement): not needed in India but requiredin China• Level 2 training (tool use, managing genotypingdata, molecular analysis) needed by India• Training the trainers is suggested• In county training essential to ensure relevantpeople can attend
  21. 21. Communities of PracticeConfusion about what this CoP• Already have global CoPs in wheat (egGraingenes)• Need lower level/less threatening CoP• Suggest starting small with a GCP wheat CoPand working upwards- post data & information on website- post comments/queries- monthly email up-date of contents toremind people to look- needs a person to coordinate

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