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GRM 2011: Phenotyping wheat for drought tolerance

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  • 1. Phenotyping for Drought Traits in WheatFrancis Ogbonnaya/Fernanda DreceerGCP AGM 21-25 Sept 2011
  • 2. Outline of PresentationGCP AGM 21-25 Sept 2011BackgroundObjectivesActivitiesTeamSome results
  • 3. BackgroundGCP AGM 21-25 Sept 2011There have been various project attempts aimed at improvingadaptation to drought.However, at the field level, advances are limited by:i. The lack of methods for reliable high-throughputphenotyping that allow season-long monitoring ofcomplex traits (e.g. crop water status or use)(Different technologies are around and used ad hoc, withno systematic calibration or link between them or withother soil, weather and ancillary crop info)ii. knowledge on which traits and trait combinations aremost relevant to different patterns of drought;(fragmentary, poor quantification across different mega-environments)In addition:It would be useful to know which traits underpinperformance of already successful germplasm underdrought, such as elite ICARDA germplasm.
  • 4. ObjectivesGCP AGM 21-25 Sept 20111. Develop and validate a field-based, low-cost, highthroughput phenotyping system with the purpose ofestimating patterns of crop water use on a seasonal basis2. Make progress towards assessing the relative impact ofputative key traits on drought adaptation by comparingrelated lines contrasting in trait value in different droughtmega-environments.3. Develop a better understanding of the potential value ofICARDA elite drought adapted lines and the traitsunderpinning them in contrasting drought environments(all project lines will be genotyped using SSR markersfrom the GCP genetic diversity kit).4. Build capability in non-invasive phenotyping methodsrelated to drought tolerant traits and the estimated valueof different traits, particularly within the Central andWest Asia and North Africa.
  • 5. ActivitiesGCP AGM 21-25 Sept 2011Progresstowardsknowledge ontrait impact onyield underdifferent typesof droughtActivity 1Activity 3Activity 2Activity 4Site characterisationPhenotypic screendevelopment andvalidationSeason longphenotypic signatureTrait comparison-phenotypingCapability building CWANA regionCharacterisationICARDA linesTraitsunderpinningdroughtadaptation inICARDA linesTrained humanresources inselection toolsand traits inCWANA regionSelection tools:highthroughputphenotypingprotocolsProducts
  • 6. Partners are based in contrastingdrought mega-environmentsGCP AGM 21-25 Sept 2011Obregon,MexicoKulumsa and Dhera,EthiopiaGatton andTemora,AustraliaSidi el Aydi,MoroccoTel Hadya andHassakieh,SyriaSummer dominant rainfallWinter dominant rainfallUniform rainfall
  • 7. ActivitiesCAIGE meetingAdelaide March 11, 2011GCP AGM 21-25 Sept 2011• Plant Materials- 200 wheat lines, including 47 to 72 contrasts forknown traits – such as early vigour, transpiration efficiency, stemsoluble carbohydrates, stay green, etc.•Environmental Characterisation- soil and weather characterisationper site•Phenotyping protocols - Common protocols were agreed on forthree areas of research activities: screen development, traitcomparison and characterisation•Project Workshop - training meeting held in Aleppo May 2010•Data Management – Translational data base where data are beingcollated
  • 8. Activities20-25 field trials between 2010 to 2011Characterized with molecular markersAt least 3 -4 tentative papersGermplasm in national variety trialsFuture MARS tool - ICARDA
  • 9. TraitsYLDYield in Kg by hectareBIO BiomassHI Harvest indexTR Threshing indexTKW Thousand Kernel weightPH Plant heightDH Date to headingDM Date to maturityDB Days to bootingDA Days to AnthesisDTDays to tilleringSEARF11PM21Number of plants, 1 week after emergence (m-2) Merc11GFP Grain filling period (or duration GFD)GCZ22 Ground cover at Zadoc scale 22GCT Ground cover at TilleringGCSE Ground cover at Stem elongationGCET Ground cover at early tilleringGCZ12 Ground cover at Zadoc scale 12GCET Ground Cover at early tilleringGCLT Ground Cover at late tilleringVIGE Vigor earlyVIGET Vigor at early tilleringVIGLT Vigor at late tilleringVIGSE Vigor at stem elongationVIGZ12Vigor at Zadoc scale12SL Spike lengthPL Peduncle lengthSPM2 Number of spike per meter squareKM2 Number of kernel by meter squareKWM2 Kernel weight per meter squareKSPKNumber of kernel per spikePW1D 1 Dcm weight from PeduncleSKW Sample kernel weightSB Sample biomassSSN Sample Spike numberSKN Sample kernel numberNDVI 1 NDVI 4days after headingNDVI 2 NDVI 15days after headingNDVI 3 NDVI25days after headingNDVI 4 NDVI 34days after headingSPAD1 SPAD 8 daya after headingSPAD2 SPAD 15 daya after headingSPAD3 SPAD 23 daya after headingSW stem WaxLW Leaf WaxLR Leaf rollingLD32DAH Leaves death 32 Days after headingLD40DAH Leaves death 40 Days after headingCTH Canopy Temperature 14-4 at HeadingATH Air Temperature 14-4 at headingDTH DIF (AIR T -Canopy T) 14-4 at headingCTBGF Canopy temperature at begining of grain filling 21-4ATBGF Air Temperature 21-4 at begining of grain fillingDTBGF DIF (AIR T -Canopy T) 21-4 at begining of grain fillingCTEGF Canopy temperature at Late of grain filling 4-5ATEGF Air Temperature 4-5 at Late of grain fillingDTEGF DIF (AIR T -Canopy T) 4-5 at Late of grain fillingFLA Flag leaf AreaFLL Flag leaf lengthFLW Flag leaf widthDLW Dry weight Flag leafSLW Specific leaf weightCTMGF Canopy temperature at the MID grain filling TH10CTEGF Canopy temperature at the end of grain filling TH10CTTL Canopy temperature at TilleringCTBT Canopy temperature at bootingSPAD SPADCTBGF Canopy temperature at begining of grain fillingCTMGF Canopy temperature at mid- grain fillingCTEGF Canopy temperature at the end of grain fillingTP Tipping percentageTD Tipping damage
  • 10. Impact-Is GCP germplasm useful for NARs?GCP AGM 21-25 Sept 2011Environment Mean 1 2 3 4BR11 2760 AUS30355 CHAM-6/SHUHA-14/73 DOBUC-1/FOW-2/125 KHATWA-4ETHDH09 335 SsrT16 KHATWA-4 DOBUC-1/FOW-2/125 CHAM-6/SHUHA-14/73ETHDH10 2249 SsrT16 KHATWA-4 DOBUC-1/FOW-2/125 CHAM-6/SHUHA-14/73ETHML09 749 SsrT16 KHATWA-4 DOBUC-1/FOW-2/125 CHAM-6/SHUHA-14/73ETHML10 2311 AUS30355 KHATWA-4 SsrT16 DOBUC-1/FOW-2/125MERC11 3215 VOROBEY CHAM-6/SHUHA-14/19 DOUMA-4 NEJMAH-16SEAIRR10 1878 AUS30355 SHIHAB-12 AUS30354 KAUZS/PREWSEAIRR11 4225 SHIHAB-12 KOUKAB-2 SOKOLL MOOWAFAQ-3SEARF10 1788 AUS30355 VOROBEY ATTILA-7 CHAM-6/SHUHA-14/19SEARF11 4473 SsrT16 CHAM-6/SHUHA-14/19 NEJMAH-16 ATTILA-7TH10 2995 AUS30355 CHAM-6/SHUHA-14/73 DOBUC-1/FOW-2/125 KHATWA-4TH11 3259 AUS30355 CHAM-6/SHUHA-14/73 KHATWA-4 DOBUC-1/FOW-2/125
  • 11. Meeting Aleppo May 2010: meeting andtraining, protocol agreementGCP AGM 21-25 Sept 2011
  • 12. ACKNOWLEDGMENTSFunding Agencies CollaboratorsBreeders:Osman AbdallaGreg RebetzkeRichard RichardsDavid BonnettMatthew ReynoldsRichard Trethowan,
  • 13. ThanksICARDA