Secured JGI CSP support for resequencing and some analysis, along with a few lines under Paterson/Hash GCP … in total anticipate: 1. whole-genome Solexa resequencing of IS3620 (guinea), S. versicolor, S. propinquum, and (separately) Rio. 2. Transcriptome resequencing of about 41 additional lines that broadly sample the diversity of S. bicolor across Africa and India No cost for sequencing, but will be some costs for data analysis and databasing.
GRM 2013: Discovery and development of alleles contributing to sorghum drought tolerance -- A Paterson (redacted)
Project title: Discovery and development of alleles contributing to sorghum drought
Main thematic targeted area: Subprogramme 2: Comparative genomics for gene
Partners, and corresponding institutions:
Andrew H. Paterson (PI), Distinguished Research Professor, Univ. Georgia
I.D.K. Atokple, Sorghum Breeder, SARI, Tamale, Ghana (email@example.com)
C. Thomas Hash, Principal Scientist (Breeding), ICRISAT, Patancheru, Hyderabad
502 324, Andhra Pradesh, India (firstname.lastname@example.org)
S.P. Mehtre, Associate Professor, Dept of Genetics and Plant Breeding, Marathwada
Agricultural University, Parbhani, Maharashtra, India
Abdalla Mohamed, Sorghum Breeder. ARC, Wad Medani, Sudan
Nadoor Seetharama, Director, National Research Center for Sorghum, Rajendranagar,
Hyderabad 500 030, Andhra Pradesh, India (email@example.com)
Sorghum is the most drought-tolerant dual-purpose (grain + straw)
cereal crop of the semi-arid tropics and subtropics, where development
challenges are the greatest and market failure is most acute.
Two major project elements:
1. Enhance terminal drought tolerance in improved sorghum
germplasm by evaluating combinations of staygreen genes.
2. Test relationships between drought tolerance and specific genes, gene
families and functional SNPs.
Fig 1. Difference in
green leaf area
flowering in two stay-
introgression lines K
260 and K293 (Stg3
donor B35 in
background of highly
senescent but elite
variety R 16)
Many, to isolate QTL, produce crosses, etc … most recently completed is ..
Ninety-one (91) double-QTL introgression homozygotes, their single-QTL
introgression parents, donor and recurrent parents, and a local check,
Kapaala were evaluated under irrigated dry season and rain-fed conditions
for their agronomic performance (Ghana).
Products, Partners, Capacity Building
Stg lines – one and two-QTL combinations
E36-1 x N13 RIL population
8 transcriptomes, associated SNPs, and inferred
-built on BTx623 genome sequence (2009, Nature), now
being used to improve primary annotation along with …
-33 more transcriptomes and 30 genomes from US DOE,
including priority genotypes of many other GCP scientists
Many trainees at numerous levels