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GRM 2013: Enhancing sorghum grain yield and quality for the Sudano-Sahelian zone of West Africa using the  Backcross Nested Association  Mapping (BCNAM) approach -- N Teme
 

GRM 2013: Enhancing sorghum grain yield and quality for the Sudano-Sahelian zone of West Africa using the Backcross Nested Association Mapping (BCNAM) approach -- N Teme

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    GRM 2013: Enhancing sorghum grain yield and quality for the Sudano-Sahelian zone of West Africa using the  Backcross Nested Association  Mapping (BCNAM) approach -- N Teme GRM 2013: Enhancing sorghum grain yield and quality for the Sudano-Sahelian zone of West Africa using the Backcross Nested Association Mapping (BCNAM) approach -- N Teme Presentation Transcript

    • Enhancing sorghum grain yield and quality for the Sudano-Sahelian zone of West Africa using the Backcross Nested Association Mapping (BCNAM) approach Niaba Teme et al.2013 Start : July 2010 End : June 2014 Related GCP project G7010-05.01 GRM-LISBON 27-30 SEPT-2013 1
    • SORGHUM STAT IN MALI 2
    • OBJECTIVES •Develop improved experimental material with enlarged genetic basis in an elites adapted background. •Phenotype improved materials in the target environments •Identify key genomic regions that accounts for phenotypic performance. •Initiate efficient molecular based breeding approaches •Enhance capacity of national breeding programs for wider use of germplasm diversity using molecular tools 3
    • COMPONENTS •Population development •Population phenotyping •Data Management and analysis • Capacity building 4
    • POPULATION DEVELOPMENT Figure 1 – parental lines crossing scheme for BC1 population development 5
    • Figure 2 - Neighbor joining tree of the BCNAM parental lines using 1320 SNPs markers POPULATION DEVELOPMENT 6
    • POPULATION DEVELOPMENT- Hand emasculation/ms3 Offseason seedling preparation Figure 3 - BC1F3 Population development offseason at Sotuba station. flowering initiation by providing short day to plant with (white boxes) 7
    • POPULATION DEVELOPMENT Figure 4. Selection pressure at BC1F2 level, 2009 rainy season. Extreme Genotypes in maturity and height are discarded, 3 plants selfed , 1 maintained for BC1F3 8 57 POPULATIONS 6300 FAMILIES-BC1F4 70-200 FAMILIES/POPULATION
    • EARLY PHENOTYPING Cinzana Sotuba BAMAKO TOMBOUCTOU GAO MOPTIKAYES SEGOU SIKASSO Kolombada Côte d’ivoire Guinée Sénégal Mauritanie Niger Burkina Faso Algérie Figure 5. phenotyping sites 9
    • EARLY PHENOTYPING- Augmented Design- Each population randomized Two dates of planting- two-three weeks apart Sites: SB1, SB2, CZ1, CZ2 Families in sub block of 10 RP is check in eack sub block. Plot = 1 row of 4m Spacing: 0.75 m between row and 0.40 m between hill 10 hills/row 150 kg/ha DAP planting time 100 kg/ha urea at 30 and 45 days after emergence 10 2150 FAMILIES
    • EARLY PHENOTYPING: > 50 TRAITS MEASURED Figure 6. Early phenotyping, SB2-2012 11
    • Figure 7: Box plot of days to flag leaf ligulation-SB1 GR//SC566-14 GR//B35 GR//CSM63 GR//Framida GR//E36-1 GR//IS23540 GR//Gadiaba GR//Konotene GR//CSM417 GR//CSM388 GR//White Kaur GR//IS15401 GR//Fara KK//B35 KK//E36-1 KK//CSM417 KK//IS15401 KK//Fara 50 70 90 110 130 KK GR Figure 8:Box plot of grain yield (kg/ha)-SB1 GR//SC566-14 GR//B35 GR//CSM63 GR//Framida GR//E36-1 GR//IS23540 GR//Gadiaba GR//Konotene GR//CSM417 GR//CSM388 GR//White Kaur GR//IS15401 GR//Fara KK//B35 KK//E36-1 KK//CSM417 KK//IS15401 KK//Fara 0 2000 4000 6000 8000 GRKK EARLY PHENOTYPING Maturity variation SB1 Grain yield variation SB1 12
    • 500 1000 1500 2000 2500 3000 3500 4000 4500 5000 5500 6000 6500 7000 7500 8000 11-1IS15401//GR 12-1Lata3//GR 2-1Fara//GR 20-1IS23540//GR 31-1B35//GR 35-1Konotene//GR 36-1SC566-14//GR 37-1Framida//GR 38-1CSM417//GR 39-1CSM63//GR 40-1CSM388//GR 41-1Gadiaba//GR 42-1WhiteKaur//GR 8-1E36-1//GR Grikan SB1 Moyenne Maximum Rendementgrainkg/ha 500 1000 1500 2000 2500 3000 3500 4000 4500 5000 5500 6000 11-2IS15401//GR 2-2Fara//GR 20-2IS23540//GR 31-2B35//GR 35-2Konotene//GR 36-2SC566-14//GR 37-2Framida//GR 38-2CSM417//GR 39-2CSM63//GR 40-2CSM388//GR 41-2Gadiaba//GR 42-2WhiteKaur//GR 8-2E36-1//GR Grinkan SB2 Moyenne Maximum Rendementgrainkg/ha GRAIN YIELD-SB1 GRAIN YIELD-SB2 EARLY PHENOTYPING Figures 8: grain yield SB 2013 13
    • 500 1000 1500 2000 2500 3000 3500 4000 4500 5000 5500 6000 6500 11-3IS15401//GR 2-3Fara//GR 20-3IS23540//GR 31-3B35//GR 35-3Konotene//GR 36-3SC566-14//GR 37-3Framida//GR 38-3CSM417//GR 39-3CSM63//GR 40-3CSM388//GR 41-3Gadiaba//GR 42-3WhiteKaur//GR 8-3E36-1//GR CSM388 CZ1 Moyenne Maximum Rendementgrainkg/ha 500 1000 1500 2000 2500 3000 3500 4000 4500 11-4IS15401//GR 2-4Fara//GR 31-4B35//GR 35-4Konotene//GR 38-4CSM417//GR 39-4CSM63//GR 40-4CSM388//GR 41-4Gadiaba//GR 42-4WhiteKaur//GR GR CZ2 Moyenne Maximum Rendementgrainkg/ha EARLY PHENOTYPING GRAIN YIELD-CZ1 GRAIN YIELD-CZ2 Figures 9: grain yield CZ-2013 14
    • EARLY YIELD TRIAL OF PROMISING BC1F5-2013 48 failies-BC1F4 Three sites: SB, CZ, FK Two rep Two row plot Figure 10: BCNAM early yield trial-SB 15
    • CAPACITY BUILDING •Training of 11 young Malians on IBP •2 PhD students in plant breeding •1 MSc in plant breeding •Trained more than 15 BSc in plant breeding and phenotyping •Irrigation infrastructure for year around plant breeding •Experimental land availability •Offices, weather stations and computers available •Seed storage facility is under construction 16
    • EARLY ONCLUSION •BC1F4 populations created important source of genetic diversity for the breeding programs. •Multi-site phenotyping is under way now and may continue in 2014 • Genotyping and QTL mapping are yet to be achieved. 17
    • FUTURE •Advancement of the BC1 population to F6-7- fund needed •Multilocal yield trial of promising families/lines •Seed increase- fund needed •Genotyping of BC1F3 families?? •Phenotypic data analysis of populations-fund need • Multi-allelic MARS scheme- Fund needed •Dual purpose phenotyping- fund needed • Capacity building- human resources 18
    • THANK YOU MERCI THANK YOU MERCI 19 Acknowledgments •GCP for the financial support and advice •IER and ICRISAT for research facilities •CIRAD for the scientific support and advice •All support staffs involved in the conduct, collect and analysis of field data