Frost & Sullivan Enabling Genomics Research Analyst Briefing

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Frost & Sullivan’s analyst briefing on the genomics research.

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Frost & Sullivan Enabling Genomics Research Analyst Briefing

  1. 1. Enabling Genomics Research: How do Platform Technologies Fit with Next Generation Applications? Jonathan Witonsky, Industry Manager Drug Discovery Technologies and Clinical Diagnostics October 29, 2008
  2. 2. Focus Points Background: Genomics Technologies and Applications e.g. Gene Expression Analysis Applications Markets: Growth Driven by Established and Emerging Trends e.g. Nucleic Acid Purification and Isolation Markets Supply-side Analysis: Product Positioning and Marketing e.g. Next Generation Sequencing Technology Demand-side Analysis: Usage of Genomics Technologies e.g. Correspondence between Technologies and Applications 2
  3. 3. Background Genomics Technologies and Applications 3
  4. 4. Background DNA Sequencing Nucleic Acid Isolation qRT-PCR Technology PCR DNA Microarray Technologies Reagents Consumables Instrumentation Software Applications Gene Expression Epigenetics Sequencing Genotyping Cytogenetics 4
  5. 5. Background (contd…) Timeline of High-growth Periods for Genomics Applications epigenetics / molecular cytogenetics (microarray) genotyping gene expression analysis de novo DNA sequencing / resequencing for SNP discovery 1998 2000 2002 2004 2006 2008 2010 2012 2014 5
  6. 6. Background (contd…) e.g. Gene Expression Analysis Typical platform technology: Typical platform technology: Real-time PCR Standard DNA Microarray Gene Expression Analysis Low density (moderate market growth) Sweet Spot for Novel Platform Medium density (high market growth) Technologies High density (moderate market growth) 6
  7. 7. Markets Growth Driven by Established and Emerging Trends 7
  8. 8. Markets 2012 Customer Priorities 1. Regulated in vitro diagnostics 2007 2. Multiple commercialized Customer Priorities applications 1. Validation 3. Research research workflows involve 2002 2. Clinical approval multiple genomics technologies 3. Forensics and Customer Priorities 4. Ultra-high infectious disease 1. Proof-of-principle testing throughput technologies 2. Basic research and discovery 8
  9. 9. Markets (contd…) e.g. Nucleic Acid Purification and Isolation Percent of Revenues by Nucleic Acid Type (U.S.), 2007 Plasmid DNA Fragment DNA 27.1% 15.4% 0% 5 – 7% Growth Growth 12 – 14% 16 – 18% Growth Growth RNA 29.7% Genomic DNA 27.8% Note: All figures are rounded; the base year is 2007. Source: Frost & Sullivan 9
  10. 10. Supply-side Analysis Product Positioning and Marketing 10
  11. 11. Supply-side Analysis Product Positioning #1 Product Positioning #3 Nucleic Acid Gene SNP qPCR qPCR Expression Genotyping Detection/ Quantitation Epigenetics Gene SNP (DNA Cytogenetics DNA Microarray Expression Genotyping Methylation, (CNP/CGH) DNA Microarray ChIP-chip) SNP Discovery Nucleic Acid de novo SNP CE Sequencing CE Sequencing Sequencing and Genotyping Detection/ Resequencing Quantitation Epigenetics Epigenetics Next Generation de novo SNP Discovery (DNA Cytogenetics Next Generation de novo SNP Discovery (DNA Cytogenetics and and Sequencing Sequencing Resequencing Methylation, (CNP/CGH) Sequencing Sequencing Resequencing Methylation, (CNP/CGH) ChIP-chip) ChIP-chip) Product Positioning #2 Product Positioning #4 Nucleic Acid Nucleic Acid Gene SNP Gene SNP qPCR Detection/ qPCR Detection/ Expression Genotyping Expression Genotyping Quantitation Quantitation Epigenetics Epigenetics SNP Discovery SNP Discovery Gene SNP (DNA Cytogenetics Gene SNP (DNA Cytogenetics DNA Microarray Expression Genotyping Methylation, (CNP/CGH) and DNA Microarray Expression Genotyping Methylation, (CNP/CGH) and Resequencing Resequencing ChIP-chip) ChIP-chip) CE Sequencing CE Sequencing Epigenetics Next Generation Next Generation de novo SNP Discovery (DNA Cytogenetics and Sequencing Sequencing Sequencing Resequencing Methylation, (CNP/CGH) ChIP-chip) 11
  12. 12. Supply-side Analysis (contd…) e.g. Next Generation Sequencing Technology Genome Sequencer Genome Analyzer SOLiD™ FLX System System Analyzer (Roche Applied Science) (Illumina, Inc.) (Applied Biosystems) • Ancient DNA • De novo Sequencing • Targeted Resequencing • ChIP-seq/Methylation/ • Whole-Genome Resequencing • Gene Expression Epigenetics • Discovery of Somatic Genomic • MicroRNA Discovery • Eukaryote Whole Genome Variation • Whole Genome Sequencing • Expression Tags • Metagenomics • HIV Sequencing • Epigenomics • Metagenomics & Microbial • Targeted Sequencing Diversity • Whole-Transcriptome Profiling • Mitochondria/ Viruses/ Plastids/ • Protein-DNA or Protein-RNA Plasmids Interactions • Prokaryote Whole Genome • Sequence Capture Targeted Region • Small RNA • Somatic Variation Detection • Transcriptome Sequencing 12
  13. 13. Demand-side Analysis Usage of Genomics Technologies 13
  14. 14. Demand-side Analysis Research Focus Area Research Group 5 tends to be a Research category in itself that Group 1 focus Group 4 can can, however, be can be pursued be pursued pursued in parallel in parallel with Research Group 3 tends to be separately or with other research all the other pursued with Group 2. together with areas of focus. areas. Group 2. Research Group 3 Research Group 2 Research Group 4 Research Group 5 Research Group 1 • Developmental • Cardiovascular • Infectious Disease/ • Cancer Research / • Gene Expression / Biology • Metabolic Microbial detection Oncology Regulation • Stem cells/ develop- • Pre-clinical studies & ID • Drug Discovery / • Gene Function mental biology • Immunology Target Identification • Protein Expression • Service provider / -central core facility Research groups 4 and 6 tend Research Group 6 to not go together. • Evolutionary Biology (negatively correlated) 14
  15. 15. Demand-side Analysis (contd…) Applications Research Group 3 tends to be a category in itself that Group 4 can be can, however, be pursued pursued in parallel Application separately or in with other Group 6 focus parallel with the applications. Group 2 can be can be pursued Research Group 5 tends to pursued separately other groups in in parallel with be pursued with Group 1. or together with the other sub- all the other Group 1. branch. applications. Application Application Application Application Application Application Group 5 Group 1 Group 2 Group 4 Group 3 Group 6 • Whole genome • SNP Discovery / • Histone • Clone • Ribosomal • Focused gene gene expression Resequencing modification identification sequencing expression profiling • Other mutation profiling / ChIP- and verification • Drug metabolism profiling • Whole genome screening chip • CDNA pharmacogenic • Novel or rare genotyping • Copy number • Regulatory sequencing profiling transcript studies variation / loss of protein factor • De novo • SAGE profiling / expression heterozygosity binding studies sequencing digital gene profiling studies • DNA Methylation expression • Microsatellite State Profiling profiling (STR) analysis • Small RNA/micro • Splice variant • Candidate gene RNA expression expression genotyping profiling profiling 15
  16. 16. Demand-side Analysis (contd…) Technologies Technology Group 2 tends to be a Technology Group 1 can category in itself that Groups 5 and 6 be adopted can, however, be can be pursued separately or adopted in parallel in parallel with Technology Group 4 tends together with with other all the other to be adopted with Group 3. Group 3. technologies. areas. Technology Technology Technology Technology Technology Technology Group 4 Group 3 Group 1 Group 2 Group 6 Group 5 • Multiplex Assay • Next generation • PCR/ Thermal • RNA • Gel Based • Microarray sequencing Cycling interference (Northern/ • Robotics / • Capillary • DNA/RNA (RNAi/siRNA) Southern / Automated electrophoresis isolation & • Transfection Western) liquid handling sequencing purification • Single molecule technologies detection • qPCR/RT-PCR platforms 16
  17. 17. Demand-side Analysis (contd…) e.g. Drivers of Adoption: Next generation sequencing Application Group 1 • SNP Discovery / Resequencing • Other mutation screening • Copy number variation / loss of heterozygosity studies 1.27 • Microsatellite (STR) analysis • Candidate gene genotyping studies Adoption of Next generation sequencing .76 Application Group 5 • Whole genome gene expression profiling • Whole genome genotyping studies 17
  18. 18. Demand-side Analysis (contd…) e.g. Drivers of Adoption: Microarray Research Group 1 • Gene Expression / Regulation • Gene Function • Protein Expression .41 Research Group 5 • Cancer Research / Oncology .32 • Drug Discovery / Target Identification Adoption of Microarray .71 Application Group 5 • Whole genome gene expression profiling • Whole genome genotyping studies .52 Application Group 6 • Focused gene expression profiling • Novel or rare transcript expression profiling 18
  19. 19. Demand-side Analysis (contd…) e.g. Correspondence between Technologies and Applications qPCR/ Focused gene This chart shows the perceived correspondence between the Candidate gene RT-PCR expression profiling various technologies (red boxes) and applications (blue circles). genotyping studies The selection applications closest to each technology are those that are most closely associated with it and those that most distinguish it from other technologies. Novel or rare transcript expression profiling PCR/ Splice variant Copy number variation/ loss thermal expression of heterozygosity studies CDNA profiling Clone identification cycling Sequencing and verification De novo sequencing Next Other generation Microsatellite Whole genome mutation sequencing (STR) analysis genotyping studies Capillary rescreening electrophoresis DNA methylation sequencing SNP discovery / DNA/RNA state profiling resequencing isolation & Multiplex Ribosomal purification SAGE Histone assay sequencing technology profiling modification Microarray profiling/ Robotics/ automated ChIP-chip liquid handling Small RNA/ micro RNA Gel Based expression profiling Single molecule Drug metabolism Whole genome gene detection platforms pharmacogenic expression profiling DNA footprinting profiling genotyping RNA interference (RNAi/ siRNA ) Regulatory protein Transfection factor binding studies 19
  20. 20. Demand-side Analysis (contd…) Conclusions • Majority of respondents are aware of most of the genomic technologies. Awareness is higher for PCR, DNA/RNA isolation, qPCR and Gel-based. It is lowest for Single molecule detection platforms and Next Gen sequencing. • Most of the genomic technologies have been already adopted by majority of the respondents. Almost universally adopted technologies include PCR, DNA/RNA isolation, qPCR, and Transfection. • Technologies with the potential for a higher rate of adoption in the next 12 months include: Microarray, RNA interference (RNAi/siRNA), Multiplex assay, Single molecule detection platforms, and Next generation sequencing. • A majority of the respondents are very satisfied with PCR and capillary electrophoresis sequencing. • In hospitals/clinical labs more than 80% are very satisfied with robotics and 70% with gel-based technologies. • Satisfaction is lowest for microarray and RNA interference. • The key reasons for satisfaction are: usefulness to research and ease of use. • The key reasons for dissatisfaction with various technologies are: difficulty of use, high operating cost, expensive or difficult sample preparation, and low accuracy. 20
  21. 21. Your Feedback is Important to Us What would you like to see from Frost & Sullivan? Growth Forecasts? Competitive Structure? Emerging Trends? Strategic Recommendations? Other? Please inform us by taking our survey. 21
  22. 22. For Additional Information • To leave a comment, ask the analyst a question, or receive the free audio segment that accompanies this presentation, please contact Stephanie Ochoa, Social Media Manager at (210) 247- 2421, via email, analystbriefings@frost.com, or on Twitter at http://twitter.com/stephanieochoa. 22

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