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Tissue Microarray presentation

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Folio Biosciences' tissue microarray product line enhances research by providing multiple patients on one array. This presentation goes into detail on how the microarrays are made and Folio's …

Folio Biosciences' tissue microarray product line enhances research by providing multiple patients on one array. This presentation goes into detail on how the microarrays are made and Folio's capabilities in this area.


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  • Transcript

    • 1. Tissue ro arraysMic
    • 2. Folio Tissue ArrayHuman Cancer Tissue Microarray 0.6 mm, 240 spotsHuman Alzheimer’s Tissue Macroarray 6mm, 8 spots
    • 3. Tissue Micro Arrays Quality Control & Assurance SOP:• Fully qualified tissues for studies and assays• Sample cores selected by Board-certified Pathologists• Every Array slide is individually read and quality checked• Every 25th slide from Array block stained and read by Pathologist• Spot size & % retention assessed (95% assured)• Quality control & verification of pathological data associated with each sample
    • 4. Tissue Arrays Top 5 problems with TMA • Poorly fixed tissues & fixative/embedding medium not optimized for detecting target molecules of interest • Detachment of specimens while processing • Heterogeneity of tissue samples • Sectioning artifacts & lack of batch-to-batch consistency • Access to relevant clinical dataData from secondary research
    • 5. Tissue Array Manufacturing Process Slide from University of Michigan
    • 6. Tissue Array ManufacturingSample Size & Mapping donor Tissue Slide from NCI
    • 7. Tissue Array Manufacturing Slide from NCI
    • 8. Tissue Array Manufacturing Donor Block Slide from NCI
    • 9. Tissue Array Manufacturing Recipient Block 0% Slide from NCI
    • 10. Folio Arrays – ISH ResultNeuritic Plaques Human Liver Cancer & Normal Adjacent tissue
    • 11. Phylogeny Tissue Array Alzheimer’s Brain Validation Data ImagesNeuritic Plaques 100x Neuritic Plaques 400x Neurofibrillary Tangles 400x
    • 12. Folio Arrays – IHC Result Alzheimer’s Parietal Cortex Normal Parietal Cortex(Amyloid Plaques seen in red color) (Amyloid Plaques absent)
    • 13. Tissue Array Manufacturing Process Tissue Grossing Preparation of Donor block Donor block sectioning IHC H & E Staining (Immunohistochemistry)Pathologist Evaluation ISH (In Situ hybridization)
    • 14. Tissue Array Design 1 2 3 4 Representative tissue A BrTu BrTu BrTu BrTu B BrNo BrNo BrNo BrNo • Disease vs Normal C ColTu ColTu ColTu ColTu • Total donors D ColNo ColNo ColNo ColNo E KidTu KidTu KidTu KidTu • Spot size F KidNo KidNo KidNo KidNo • Spot number G OvTu OvTu OvTu OvTu • Duplicates/ H OvNo OvNo OvNo OvNo triplicates I LuTu LuTu LuTu LuTu J LuNo LuNo LuNo LuNo • Section thickness K LivTu LivTu LivTu LivTu L LiVNo LivNo LivNo LivNo# Coordinates Sex Age Tumor Type Donor Pathological Diagnosis135 G 4 F 66 Ovary Tumor OV-218 Ovary:mucinous borderline (low malignant potential) tumor with areas of intraglandular carcinoma135 G 19 F 53 Ovary Tumor OV-803 Ovary: Serous carcinoma, bilateral, tumor grade high, nuclear 2 to 3/3)136 G 14 F 64 Ovary Tumor OV-588 Ovary: Adenocarcinoma grade 2, moderately differentiated