Virus Super Resolution Microscopy TMV
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Virus Super Resolution Microscopy: A further example for the use of localization microscopy/SPDM is an analysis of Tobacco mosaic virus (TMV) particles. TMV particles are not only important in plant ...

Virus Super Resolution Microscopy: A further example for the use of localization microscopy/SPDM is an analysis of Tobacco mosaic virus (TMV) particles. TMV particles are not only important in plant research but are also an example for a class of most promising biomolecule complexes exhibiting a high potential for nanotechnology applications as virus-derived biotemplates for nanostructured hybrid materials and building-blocks in analytical, technical & therapeutic applications.

Collaboration of the Cremer & the Wege labs (Universities of Heidelberg & Stuttgart).
Reference: Superresolution imaging of biological nanostructures by spectral precision distance microscopy (2011): Cremer, R. Kaufmann, M. Gunkel, S. Pres, Y. Weiland, P- Müller, T. Ruckelshausen, P. Lemmer, F. Geiger, S. Degenhard, C. Wege, N- A. W. Lemmermann, R. Holtappels, H. Strickfaden, M. Hausmann , Biotechnology 6: 1037–1051

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  • Rainer Kaufmann, Patrick Müller, Georg Hildenbrand, Michael Hausmann & Christoph Cremer: Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy, Journal of Microscopy 2010,

Virus Super Resolution Microscopy TMV Presentation Transcript

  • 1. Virus super resolution microscopyLocalisation microscopy: SPDMphymod images of Tobacco mosaic virus (TMV) particles withcoated proteins labeled with the fluorophore Atto448.B: after Gaussian blurring corresponding to the individual localization accuracyD: same data, but distribution of the individual molecule position without Gaussain blurringCollaboration of the Cremer & the Wege labs (Universities of Heidelberg & Stuttart). Superresolution imaging of biological nanostructuresby spectral precision distance microscopy (2011): Cremer, R. Kaufmann, M. Gunkel, S. Pres, Y. Weiland, P- Müller, T. Ruckelshausen, P.Lemmer, F. Geiger, S. Degenhard, C. Wege, N- A. W. Lemmermann, R. Holtappels, H. Strickfaden, M. Hausmann , Biotechnology 6: 1037–