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Nitrogen use and climate change mitigation  Liz Baggs University of Aberdeen
Short History of Nitrous Oxide Joseph Priestley 1775  N 2 O
Joseph Priestley 1775  Short History of Nitrous Oxide N 2 O
Ode to Nitrous Oxide "Yet are my eyes with sparkling lustre fill'd Yet is my mouth replete with murmuring sound Yet a...
Short History of Nitrous Oxide
Short History of Nitrous Oxide
Nitrous Oxide is a Potent Greenhouse Gas CO 2 N 2 O CH 4 Carbon Dioxide Methane Nitrous Oxide 20 years 100 years 500 years...
Atmospheric nitrous oxide has increased by 20% over the last 100 years N 2 O concentrations  (IPCC Fourth Assessment Repor...
 
 
Soil is a significant source of N 2 O 10.2 Tg N y -1 Source: IPCC (2007) IPCC 2007: ‘Land surface properties and land-atmo...
The Nitrogen Cycle NO 3 - N 2 NO 2 - NH 4 + NH 2 OH N 2 O NO D E N I T R I F I C A T I O N N I T R I F I C A T I O N
The Nitrogen Cycle NO 3 - N 2 NO 2 - NH 4 + NH 2 OH N 2 O NO D E N I T R I F I C A T I O N FIXATION
NO 3 - N 2 NO 2 - NH 4 + NH 2 OH N 2 O NO D E N I T R I F I C A T I O N N I T R I F I C A T I O N FIXATION Cellular toxin ...
Field & lab experimentation
<ul><li>spatially and temporally heterogeneous  </li></ul><ul><li>Interactions between microbiology, environment & biogeoc...
Primary controls of N 2 O production Understanding the controls of N 2 O fluxes is essential for modelling, up-scaling and...
Primary controls of N 2 O production N application Understanding the controls of N 2 O fluxes is essential for modelling, ...
Primary controls of N 2 O production Aeration/water content N application Understanding the controls of N 2 O fluxes is es...
pH Primary controls of N 2 O production Aeration/water content N application Understanding the controls of N 2 O fluxes is...
pH Primary controls of N 2 O production Aeration/water content N application Temperature Temperature ( o C) N 2 O flux (g ...
pH Primary controls of N 2 O production Aeration/water content N application Temperature Available C Understanding the con...
N 2 O is produced in several microbial processes  NH 3 NH 2 OH NO 2 - NO N 2 O N 2 NO 3 - NO 2 - NO N 2 O N 2 N 2 O Nitrif...
Gross nitrification  mg N kg -1  d -1 SRC Willow 12.0 ± 0.6 10 days Miscanthus 7.3 ± 0.3 Sandy loam NH 4 NO 3  at 12 g N m...
Denitrifier-N 2 O & N 2 15 N 10 atom %
O 2  gradient Air flow controller O 2  analyser C exudate A C exudate B Different denitrifier  gene copy numbers? 15 N-N 2...
Alleviation of Cu-limitation at pH 7? N 2 O:N 2 ?  Potential for enhancing N 2 O reduction
Common exudation compounds from Ectomycorrhizal fungi. <ul><ul><li>K 15 NO 3 , 5 g N m -2 , 10 atom % excess  15 N. </li><...
In situ  visualisation of pseudomonads  marked with  unstable  gfp  in the rhizosphere of a barley seedling Colonising roo...
Blue =  28 Si - Green =  12 C 14 N -  (represents organic matter)  Red =  15 / 14 N ratio images (distribution of  15 N en...
13 C  SOM Hotspots of denitrifier activity (e.g. with C quantity, quality & O 2  availability) N 2 O production & source p...
Manipulating the rhizosphere for function Lower N application 13 C  SOM N 2 O N 2 O:N 2 Nitrification + Denitrification Ni...
Future challenge:  Resolving   issues of scale gene plant field landscape 10 -8  m 10 -2  m 10 2  m 10 5  m Bug to big Mod...
Management for mitigation Opportunities for mitigation Controlled release fertilisers. Synchronising N applications & N av...
New opportunities for mitigation? Plant breeding for exudate C compounds which maximise reduction of N 2 O to N 2   Applic...
Greater understanding of regulation of the N 2 O reductase:  mitigation by reducing N 2 O to N 2 ? Greater understanding o...
The Nitrous Oxide Focus Group is a consortium-based research initiative established to explore the action of the greenhous...
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Nitrogen Use & Climate Change Mitigation - Liz Baggs (University of Aberdeen)

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Transcript of "Nitrogen Use & Climate Change Mitigation - Liz Baggs (University of Aberdeen)"

  1. 1. Nitrogen use and climate change mitigation Liz Baggs University of Aberdeen
  2. 2. Short History of Nitrous Oxide Joseph Priestley 1775 N 2 O
  3. 3. Joseph Priestley 1775 Short History of Nitrous Oxide N 2 O
  4. 4. Ode to Nitrous Oxide &quot;Yet are my eyes with sparkling lustre fill'd Yet is my mouth replete with murmuring sound Yet are my limbs with inward transports fill'd And clad with new-born mightiness around.&quot; Sir Humphry Davy Presidente de la Royal Society 1820-27 Short History of Nitrous Oxide N 2 O
  5. 5. Short History of Nitrous Oxide
  6. 6. Short History of Nitrous Oxide
  7. 7. Nitrous Oxide is a Potent Greenhouse Gas CO 2 N 2 O CH 4 Carbon Dioxide Methane Nitrous Oxide 20 years 100 years 500 years 1 1 1 62 23 7 275 296 156
  8. 8. Atmospheric nitrous oxide has increased by 20% over the last 100 years N 2 O concentrations (IPCC Fourth Assessment Report, 2007)
  9. 11. Soil is a significant source of N 2 O 10.2 Tg N y -1 Source: IPCC (2007) IPCC 2007: ‘Land surface properties and land-atmosphere interactions that lead to radiative forcing are not well quantified’. <ul><li>Upturn in N 2 O production due to </li></ul><ul><li>increases in soil N availability: </li></ul><ul><ul><li>N deposition </li></ul></ul><ul><ul><li>N fertilization </li></ul></ul>
  10. 12. The Nitrogen Cycle NO 3 - N 2 NO 2 - NH 4 + NH 2 OH N 2 O NO D E N I T R I F I C A T I O N N I T R I F I C A T I O N
  11. 13. The Nitrogen Cycle NO 3 - N 2 NO 2 - NH 4 + NH 2 OH N 2 O NO D E N I T R I F I C A T I O N FIXATION
  12. 14. NO 3 - N 2 NO 2 - NH 4 + NH 2 OH N 2 O NO D E N I T R I F I C A T I O N N I T R I F I C A T I O N FIXATION Cellular toxin Greenhouse gas The Nitrogen Cycle
  13. 15. Field & lab experimentation
  14. 16. <ul><li>spatially and temporally heterogeneous </li></ul><ul><li>Interactions between microbiology, environment & biogeochemistry </li></ul><ul><li>wide range of scales </li></ul>Soil: A complex environment
  15. 17. Primary controls of N 2 O production Understanding the controls of N 2 O fluxes is essential for modelling, up-scaling and mitigation
  16. 18. Primary controls of N 2 O production N application Understanding the controls of N 2 O fluxes is essential for modelling, up-scaling and mitigation grass arable-cereal arable-non cereal Baggs et al 2000 Soil Use Manage 16, 82-87. Baggs et al 2003 Plant Soil 254, 361-370. Wheat Maize Cabbage Sprouts Mustard Broccoli Sugar beet leaf Grass/clover Bean Lettuce Velthof et al 2002 Baggs et al 2000, 2003
  17. 19. Primary controls of N 2 O production Aeration/water content N application Understanding the controls of N 2 O fluxes is essential for modelling, up-scaling and mitigation nitrification denitrification
  18. 20. pH Primary controls of N 2 O production Aeration/water content N application Understanding the controls of N 2 O fluxes is essential for modelling, up-scaling and mitigation
  19. 21. pH Primary controls of N 2 O production Aeration/water content N application Temperature Temperature ( o C) N 2 O flux (g N 2 O-N ha -1 d -1 ) Understanding the controls of N 2 O fluxes is essential for modelling, up-scaling and mitigation
  20. 22. pH Primary controls of N 2 O production Aeration/water content N application Temperature Available C Understanding the controls of N 2 O fluxes is essential for modelling, up-scaling and mitigation 14 days N 2 O N 2
  21. 23. N 2 O is produced in several microbial processes NH 3 NH 2 OH NO 2 - NO N 2 O N 2 NO 3 - NO 2 - NO N 2 O N 2 N 2 O Nitrification Denitrification Nitrifier denitrification Nitrate ammonification NH 4 + N 2 O ? 15 N site preference 15 N, 18 O enrichment & natural abundance Which process is contributing to emissions under particular environmental conditions or management? N N O N N co-substrate Co- Recent advances
  22. 24. Gross nitrification mg N kg -1 d -1 SRC Willow 12.0 ± 0.6 10 days Miscanthus 7.3 ± 0.3 Sandy loam NH 4 NO 3 at 12 g N m -2 nitrification nitrate reduction Nitrification versus nitrate reduction
  23. 25. Denitrifier-N 2 O & N 2 15 N 10 atom %
  24. 26. O 2 gradient Air flow controller O 2 analyser C exudate A C exudate B Different denitrifier gene copy numbers? 15 N-N 2 O 15 N-N 2 CuNir, cdNir, NosZ pump Different denitrifier gene copy numbers? N 2 O/O 2 CuNir, cdNir, NosZ CuNir, cdNir, NosZ Lab soil columns
  25. 27. Alleviation of Cu-limitation at pH 7? N 2 O:N 2 ? Potential for enhancing N 2 O reduction
  26. 28. Common exudation compounds from Ectomycorrhizal fungi. <ul><ul><li>K 15 NO 3 , 5 g N m -2 , 10 atom % excess 15 N. </li></ul></ul>3.6 g C l -1 14 days Does C influence N 2 O reduction? 70% WFPS N 2 O N 2 Differences in regulation of NO & N 2 O reductases? Preference for different C compounds in rhizosphere denitrifier community?
  27. 29. In situ visualisation of pseudomonads marked with unstable gfp in the rhizosphere of a barley seedling Colonising root tip On root surface Where is C flowing in the rhizosphere?
  28. 30. Blue = 28 Si - Green = 12 C 14 N - (represents organic matter) Red = 15 / 14 N ratio images (distribution of 15 N enriched P. fluorescens ) Herrmann et al 2007 Rapid Comm Mass Spec 21, 29-34 Mapping location of active microbes
  29. 31. 13 C SOM Hotspots of denitrifier activity (e.g. with C quantity, quality & O 2 availability) N 2 O production & source partitioning in situ. Air filled pore Water filled pore Anoxic zone Oxic zone 15 N & 13 C in denitrifier or 15 N-N 2 O 15 N in denitrifier or 15 N-N 2 O 15 N-NO 3 applied to soil Mapping location of active microbes
  30. 32. Manipulating the rhizosphere for function Lower N application 13 C SOM N 2 O N 2 O:N 2 Nitrification + Denitrification Nitrifier denitrification Net CH 4 Inhibition of CH 4 oxidation CH 4 oxidation Distance from root/time High N application Denitrification Lowered nitrifier denitrification Denitrification Plant breeding for exudate C compounds which enhance reduction of N 2 O to N 2 SOM management to alleviate Cu-limitation to enhance reduction of N 2 O to N 2 Lower N 2 O:N 2
  31. 33. Future challenge: Resolving issues of scale gene plant field landscape 10 -8 m 10 -2 m 10 2 m 10 5 m Bug to big Modelling
  32. 34. Management for mitigation Opportunities for mitigation Controlled release fertilisers. Synchronising N applications & N availability with crop demand. Nitrification inhibitors. Split fertiliser applications. Minimise fallow periods. ‘ Optimise’ tillage. Placement of injection of fertilisers. Large, less frequent irrigation. Mosier 1994 Fert Res 37, 191-200 Mosier et al 1996 Plant & Soil 181, 95-108 Dalal et al 2003 Aust J Soil Res 41, 165-195 Residue management – combined inorganic/organic-N applications.
  33. 35. New opportunities for mitigation? Plant breeding for exudate C compounds which maximise reduction of N 2 O to N 2 Application of zeolite + lime 5 μm Nitrosomonas europaea cells attached onto clinoptilolite particles Management for mitigation Biosensor luminescence response to root exudation Paterson et al. J. Exp. Bot. 2006 57:2413-2420 Zaman et al 2007 Aust J Soil Res 45, 543-553
  34. 36. Greater understanding of regulation of the N 2 O reductase: mitigation by reducing N 2 O to N 2 ? Greater understanding of interactions with C cycle. Understanding control of microsite structures on microbial community composition & processes. Advancing techniques & adopting interdisciplinary approaches to quantify and understand controls on N 2 O. Tackling issues of scale. Integrating chemostat and soil studies to field/landscape. How can we constrain the soil-N 2 O budget? Enhance quantification and understanding of N 2 O production informing targeted and sustainable management for mitigation
  35. 37. The Nitrous Oxide Focus Group is a consortium-based research initiative established to explore the action of the greenhouse gas, Nitrous Oxide; its role in climate change, the role of bacteria in the greenhouse gas emissions and to develop techniques to mitigate its effect. Ultimately the Group will work toward solutions for the wider community and commercial and non-academic partners are being sought to inform and enable the development of opportunities arising from the Nitrous Oxide Focus Group’s research. http://www.nitrousoxide.org/index.html [email_address] [email_address]
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