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(c) Kesha Marie Tallo

(c) Kesha Marie Tallo

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Method Presentation Transcript

  • 1. Method Indicator Rgt Stability Specimen Assay Performance Normal Values Notes oGlucose Enzymatic Quinoneimine 2-8 C – up to Serum, plasma WL= 500 nm Linear up to glucose conc of Serum,plasma 1.RGT contains .095% oxidation in the dye(red-violet) expiry date *glucose is or Hg 546 400 mg/dl or 22.2 mmol/L (fasting) : Na azide o presence of 15-25 C – 2 stable for nm 2. icteric sera o glucose oxidase weeks 24hrs at 2-8 C 75-115 mg/dl interfere with test if prep within OP= 1 cm Or TAG up to 2500 30 min after 4.2-6.4 mmol/L mg/dl, haemoglobin o o collection T = 20-25 C up to 500 mg/dl and o or 37 ascorbic acid up to 20 mg/dl do not interfere 3. slight brownish sediment as RGT ages, do not swirl oTriglycerides Enzymatic Quinoneimine 2-8 C – up to Serum, WL= 500 nm Linear up to TAG conc of Atherosclerotic risk: 1.RGT contains .05% hydrolysis with dye expiry date heparinised or Hg 546 1000 mg/dl or 11.4 mmol/L Suspect: >150 mg/dl Na azide o lipases 20-25 C – 4 plasma or nm or 1.71 mmol/L 2. not influenced by weeks EDTA plama haemoglobin values *STABILITY: OP= 1 cm Increased: >200 up to 150 mg/dl; o 3 days @ 2-8 C mg/dl or 2.28 bilirubin up to 40 o o o 4 mos @ -20 C T = 20-35 C mmol/L mg/dl; ascorbate o *NOTE: lipemic or 37 C may give falsely low subs usually values at >4 mg/dl generate turbidity  falsely elevated LCF clears up turbidity oCholesterol Enzymatic Quinoneimine 2-8 C – up to Serum, WL= 500 nm Linear up to cholesterol Suspect: over 220 1.not influenced by hydrolysis and expiry date heparinised or or Hg 546 conc of 750 mg/dl or 19.3 mg/dl or 5.7 mmol/l haemoglobin up to o oxidation 15-25 C – 2 EDTA plasma nm mmol/l 200 mg/dl or weeks Increased: over 260 bilirubin up to 5 OP= 1 cm mg/dl or 6.7 mmol/l mg/dl 2.contain 0.05% Na o o T = 20-35 C *EAS: azide o or 37 C Adults up to 30 yrs= 180 mg/dl Over 30 yrs = 200 mg/dl
  • 2. HDL Cholesterol Chylomicrons, PREC Serum, WL= 500 nm HDL MEN WOMEN 1.if supernatant is o VLDL,LDL are 2-25 C – up heparinised or or Hg 546 Cholesterol clear (high TAG ppt’d by to expiry date EDTA plasma nm mg/dl mmol/l mg/dl mmol/l level), dilute sample addition of Prognostically before ppt phosphotungstic OP= 1 cm favourable > 55 > 1.42 > 65 > 1.68 2. high conc of acid and ascorbic acid (. 2.5 o o magnesium T = 20-35 C Standard risk 0.9 – 1.16 – mg/dl) will give lower o chloride. After or 37 C level 35-55 1.42 45-65 1.68 values centrifugation 3. haemoglobin > 100 supernatant Risk indicator mg/dl and bilirubin > contains HDL <35 < 0.9 < 45 < 1.16 10 mg/dl interfere LDL Cholesterol Suspicious: 150 mg/dl or 3.9 mmol/l Elevated: 190 mg/dl or 4.9 mmol/l oTotal Protein Cupric ions react 2-25 C – up Serum, WL= 500 nm Linear up to CHON conc of < 3 y.o 1.color rgt contains with CHON in to expiry date heparinised or or Hg 546 12 g/dl or 120 g/l 4.6 – 7.0 g/dl NaOJ alkaline sol’n to EDTA plasma nm 46 – 70 g/l 2. contains 0.095% form purple Na azide as complex. The *STABILITY: OP= 1 cm > 3 y.o preservative o absorbance of 2-8 C – up to 1 6.6 – 8.7 g/dl 3. slight sediment as o o this complex is month T = 20-25 C 66 – 87 g/L RGT ages, do not proportional to include this o the CHON conc 15-25 C – up 4. sample blank for to 1 week clear, colorless sera = 0.2 g/dl, can be ignored Sample blank must be determined for visibly haemolytic, icteric and lipemia sera by pipetting 20 ul sample to 1000 ul physiological saline and measuring against dH2O. Absorbance sample - Absorbance of blank