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  2. 2. Copyright © 2008, New Age International (P) Ltd., PublishersPublished by New Age International (P) Ltd., PublishersAll rights reserved.No part of this ebook may be reproduced in any form, by photostat, microfilm, xerography,or any other means, or incorporated into any information retrieval system, electronic ormechanical, without the written permission of the publisher. All inquiries should beemailed to rights@newagepublishers.comISBN (13) : 978-81-224-2931-2PUBLISHING FOR ONE WORLDNEW AGE INTERNATIONAL (P) LIMITED, PUBLISHERS4835/24, Ansari Road, Daryaganj, New Delhi - 110002Visit us at www.newagepublishers.com
  3. 3. This book is dedicated toPROF. B.G. SHIVANANDA Principal Al-Ameen College of Phar macy Pharmacy BANGALOREFor his Her culean effor ts in bringing Herculean effor forts APTI to a high pedestal
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  5. 5. FOREWORD Multiple choice questions (MCQs) test a candidates ability to apply his or her knowledge acquired during theregular course of study. Framing a question paper based on MCQs is time consuming but evaluating the answers iseasy.subjectiveness of the examiner associated with evaluation of essay type of answers is inherent in the evaluationprocess & depends upon several variables such as hand writing, methods of presentation etc. These variables do notexist during evaluation of answers based on MCQs. I am of the opinion that for the examination system at the undergraduate level, the entire testing of theoreticalknowledge should be MCQ based since the evaluation can be computerized & human bias can be largely eliminated. In this book, the multiple choice questions have been prepared with great care such that the questions framed areprecise & clear enabling the reader to make correct choices. A wide coverage of topics is given. I strongly recommend this book for all related to Microbiology & College Libraries. PROF DR. KANTI GOR Vice Chancellor K.S. K. V. Kachchh University Bhuj, Gujarat
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  7. 7. PREFACE The book is primarily meant for students appearing for PG competitive examinations. In USMLE, GATE, AFMC,AIIMS & other Medical, Paramedical entrance examinations for admissions to PG Programme, Microbiology is one ofthe important component of the syllabus. The main objective of this book is to help students to review their knowledge of Microbiology acquired throughstandard textbooks. A sound knowledge of Microbiology is essential for students of Medicine, Pharmacy, Dentistry &Nursing for understanding the subject with logical reasoning. This book is specially designed to complement anystandard microbiology textbook and to provide the students with a feedback on their progress & an opportunity toimprove. Thus the book can serve as a self assessment guide. With the explosion of knowledge in medical sciences, examinations in all faculties (Medicine, Pharmacy, Dentistry& Nursing) is completely becoming MCQ oriented because this system of assessment is more accurate, reliable &quicker. A welcome trend in this direction is already discernible Both the teachers & students of microbiology will find this book useful. A quick persuation of the questions willprovide evidence that the book intends to stimulate reasoning Suggestions & criticism about the book are welcome. G. Vidya Sagar
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  9. 9. ACKNOWLEDGEMENTS It gives me pleasure to acknowledge with deep appreciation for all those who have extended their co-operationduring the preparation of the book. • Prof. Dr. B. Suresh President, Pharmacy Council of India • Sri. Harish Padh Director, Sri B.V. Patel PERD centre Ahmedabad, Gujarat • Prof. Dr. Ajay K. Saluja A.R. College of Pharmacy Vallabh Vidyanagar Gujarat • Dr. E. Bhanoji Rao Principal, Rolland Institute of Pharmaceutical Sciences Behrampur, Orissa • Dr. K. Senthil Kumar Principal, Padmavathy College of Pharmacy Dharmapuri, Tamilnadu Finally, I express my gratitude to Mr. Soumya Gupta, MD, Manager, New Age International (P) Limited, New Delhi,for his encouragement and support. - Author
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  13. 13. CHAPTER 1 HISTORY OF MICROBIOLOGY1. According to Pasteur statements which 5. Who demonstrated that open tubes of one of the following is true broth remained free of bacteria when air was free of dust. a. Living organisms discriminate between stereoisomers a. Abbc Spallanzani b. John Tyndall b. Fermentation is a aerobic process c. Francisco Redi d. Pasteur c. Living organisms doesn’t discriminate 6. Reverse isolation would be appropriate between stereoisomers for d. Both a and b a. a patient with tuberculosis2. “I found floating therin earthly particles, b. a patient who has had minor surgery some green streaks, spirally wound c. a patient with glaucoma serpent-wise, and orderly arranged, the d. a patient with leukemia whole circumstance of each of these streaks was abut the thickness of a hair 7. The symptome “ general feeling of illness and discomfort “ is called on one’s head”…. These words are of a. Cystitis b. Malaise a. Leeuwenhoek b. A. Jenner c. Anaphylactic shockd. Arthritis c. Pasteur d. Koch 8. On soybean which of the following forms3. The principle light- trapping pigment mol- symbiotism ecule in plants, Algae, and cyanobacteria is a. Azatobactor paspali b. Rhizobium a. Chlorophyll a b. Chlorophyll b c. Nostoc c. Porphyrin d. Rhodapsin d. Bradyrhizobium4. During Bio Geo chemical cycle some 9. Who provide the evidence that bacterioph- amount of elemental carbon was utilized age nucleic acid but not protein enters the by the microorganisms. The phenome- host cell during infection non is called as a. Alfred D.Hershey & Leonard Tatum in 1951. a. Dissimilation b. Alfred D.Hershey & Zindar Lederberg in 1951. b. Immobilization c. Alfred D.Hershey & Martha Chase in 1952. c. Decomposition d. Alfred D.Hershey & Macleod in 1952. d. Neutralization
  14. 14. MCQs IN MICROBIOLOGY10. Spirulina belongs to 19. The image obtained in a compound a. Xanthophyceae b. Cyanophyceae microscope is c. Rhodophyceae d. Pheophyceae a. Real b. Virtual c. Real inverted d. Virtual inverted11. The first antibody to contact invading microorganisms was 20. Enzymes responsible for alcoholic a. IgG b. IgM fermentation c. IgA d. IgD a. Ketolase b. Zymase c. Peroxidase d. Oxidase12. The light emitted by luminescent bacteria is mediated by the enzyme 21. Which type of spores are produced sexually? a. Coenzyme Q a. Conidia b. Sporangiospores b. Luciferase c. Ascospores d. None of these c. Lactose dehydrogenase 22. Bacterial transformation was discovered d. Carboxylase reductase by13. Pick out the vector using in human a. Ederberg and Tatum Genome project b. Beadle and Tatum a. Phagemid vector c. Griffith b. Yeast artificial chromosomes d. None of these c. Cosmid vectors 23. Father of microbiology is d. Yeast episomal plasmids a. Louis Pasteur b. Lister14. Salt and sugar preserve foods because c. A.V. Leeuwenhock d. Robert Koch they a. Make them acid 24. The antiseptic method was first demon- strated by b. Produce a hypotonic environment c. Deplete nutrients a. Lwanowski b. Lord Lister d. Produce a hypertonic environment c. Edward Jenner d. Beijerinck15. In a fluorescent microscope the objective 25. Small pox vaccine was first discovered by lens is made of a. Robert Koch b. Louis Pasteur a. Glass b. Quartz c. Lister d. Edward Jenner c. Polythene d. None of these 26. The term mutation was coined by16. Fixation of atmospheric nitrogen is by a. Pasteur b. Darwin means of c. Hugo devries d. Lamark a. Biological process b. Lightining 27. Compound microscope was discovered by c. Ultraviolet light d. All of the above a. Antony von b. Pasteur17. Which one of the following fungi is the c. Johnsen & Hans d. None of these most serious threat in a bone marrow transplant unit? 28. Father of Medical Microbiology is a. Candida albicans b. Aspergillus a. Pasteur b. Jenner c. Blastomyces d. Cryptococus c. Koch d. A.L.Hock18. Direct microscopic count can be done with 29. Disease that affects many people at the aid of different countries is termed as a. Neuberg chamber b. Anaerobic chamber a. Sporadic b. Pandemic c. Mineral oil d. Olive oil c. Epidemic d. Endemic
  15. 15. HISTORY OF MICROBIOLOGY ! 30. Prophylaxis of cholera is 39. Tuberculosis is a a. Protected water supply a. Water borne disease b. Environmental sanitation b. Air borne disease c. Immunization with killed vaccines c. Food borne disease d. All of these d. Atthropod borne disease 31. In electron microscope, what material is 40. Phagocytic phenomenon was discovered by used as an objective lense? a. Louis Pasteur b. Alexander Fleming a. Magnetic coils c. Metchnikof d. Robert Koch b. Superfine glass 41. Meosomes are also known as c. Aluminium foils d. Electrons a. Mitochondria b. Endoplasmic reticulum 32. The main feature of prokaryotic organism c. Plasmids is d. Chondroids a. Absence of locomotion b. Absence of nuclear envelope 42. Hybridoma technique was first discovered by. c. Absence of nuclear material d. Absence of protein synthesis a. Kohler and Milstein b. Robert Koch 33. The stalked particles on the cristae of c. ‘D’ Herelle mitochondria are called d. Land Steiner a. Glyoxysomes b. Peroxisomes c. Oxysomes d. Spherosomes 43. The minimum number of bacteria required to produce clinical evidence of 34. Antiseptic methods were first introduced death in a susceptible animal under by standard condition is called a. Lord Lister b. Iwanowski a. LD50 b. ID c. Beijernick d. Edward Jenner c. MLD d. All of these 35. Kuru disease in Humans is caused by 44. In Electron Microscope source of electrons a. Bacteria b. Viroides is from c. Prions d. Mycoplasma a. Mercury lamp b. Tungsten metal 36. A mutation that produces termination c. both a and b d. None of these codon is 45. Griffith (1928) reported the phenomenon a. Mis-sense mutation of transformation first in b. Neutral mutation a. H. influenzae b. Bacillus species c. Non-sense mutation c. Pneumococci d. E.coli d. Reverse mutation 46. The resolution power of the compound 37. During conjunction the genetic material microscope is will be transferred through a. 0.2 micron b. 0.2 millimeter a. Cell wall b. Medium c. 0.2 Angstrom units d. 0.2 centimeter c. Pili d. Capsule 47. The capacity of a given strain of microbial 38. Antiseptic surgery was discovered by species to produce disease is known as a. Joseph Lister b. Ernest Abbe a. Pathogen b. Virulence c. Pasteur d. Beijerink c. Infection d. None of these
  16. 16. " MCQs IN MICROBIOLOGY48. Monoclonal antibodies are associated 58. The causative organism of rocky mountain with the name of spotted fever was first described by a. Burnet b. Medwar a. Howard Ricketts b. da Rocha-lima c. Milstein kohler d. Owen c. Both a and b d. Robert Koch49. Lederberg and Tatum (1946) described 59. The term bacteriophage was coined by the phenomena of a. De’Herelle b. F.W. Twort a. Conjunction b. Transformation c. Beijernick d. Jwanosky c. Mutation d. Plasmids 60. Viral infection of bacteria was discovered50. Hanging drop method for motility study by was first introduced by a. De’Herelle b. F.W. Twort a. Robert Koch b. Louis Pasteur c. Beijernick d. Jwanoksy c. Jenner d. Leeuwenhock 61. Eye cannot resolve any image less than51. Electron microscope gives magnification a. 1ìm b. 2ìm upto c. 7ìm d. 5ìm a. 100 X b. 2000 X 62. Compound Microscope was discovered by c. 50,000 X d. 2,00,000 X a. A.V. Lewenhoek b. Pasteur52. Term vaccine was coined by c. Janssen and Hans d. None of these a. Robert Koch b. Pasteur 63. Electron Microscope was discovered by c. Needham d. None of these a. Prof. Fritz b. Janssen and Hans53. The inventor of Microscope is c. Knoll and Ruska d. None of these a. Galileo b. Antony von 64. Magnification range of light microscope is c. Pasteur d. Koch a. 1000x – 5000x b. 1000x – 2000x54. First Pasteur conducted fermentation c. 500x – 1000x d. None of these experiments in 65. Condensation of light in light Microscope a. Milk b. Food material is by c. Fruit juices d. Both a and c a. Objective b. Condensor c. Ocular d. All of these55. Modern concepts of chemotherapy was proposed by 66. Light gathering capacity of Microscope is a. Paul Ehrlich b. Joseph Lister called c. Elie Metchnikoff d. None of these a. Numerical aperture b. Angular aperture c. Both a and b d. None of these56. The role of phagocytosis was discovered by 67. If 10x and 40x objectives are used (air is the medium), the numerical aperture is a. Paul Ehrlich b. Joseph lister a. 1.5 b. 2.0 c. Elie Metchikoff d. Pasteur c. 1.0 d. 1.857. L – forms are discovered by 68. The ability of Microscope to distinguish a. Klein Berger two objects into two separate objects, is b. Louis Pasteur called. c. Robert Koch a. Resolving power b. Wave length d. Antony von Leeuwenhock c. N.A. d. None of these
  17. 17. HISTORY OF MICROBIOLOGY # 69. Limit of resolution of compound micro- 79. Primary mediators in anaphylaxis scope is a. Histamine b. Seratonin a. 0.018 Ao b. 0.1 mm c. Heparin d. All of these c. 5 ìm d. 1 mm 80. Arthus reaction was discovered by 70. Source of light in fluorescence microscopy a. Marrice Arthus b. Von Perquit is from c. Richet d. Porter a. Mercury lamp b. Sunlight 81. Serum sickness reaction was discovered c. Both a and b d. None of these by 71. Who perfected a magnetic lens in 1927 a. Marrice Arthus b. Von perquit a. Gabor b. Broglie c. Richet d. Porter c. Busch d. None of these 82. Hybridoma technique was developed by 72. The magnefication power of electron a. Kochler & Milston b. Niel’s Jerne microscope developed by Knell and c. Both a and b d. None of these Ruska is a. 10,000x b. 12,000x 83. Disease that effects many people at different countries is termed as c. 15,000x d. 20,000x a. Sporadic b. Pandemic 73. In electron microscope source of electrons c. Epidemic d. Endemic is from a. Mercury lamp b. Tungsten metal 84. If the vectors transmit the infection mechanically they are called c. Both a and b d. None of these a. Biological vectors 74. The electron passed out from the b. Mechanical vectors specimen are called c. Biological reservoir a. Primary electrons b. Secondary electrons d. Both a and c c. Tertiary electrons d. None of these 85. If a person can be infected by direct 75. Mycorrhiza was first observed by contact with infected tissue of another a. Funk b. Frank person, it is termed as c. Fisher d. Crick a. Indirect contact transmission b. Attachment 76. The transfer of genetic material during transformation is proved basing on c. Direct contact transmission Griffith’s experiment by d. None of these a. Avery Macleod & Mc.Carthy 86. Reduction of virulence is known as b. Lederberg & Taulum a. Exaltation b. Attenuation c. Zinder & Lederberg c. Both a and b d. None of these d. Watson & Crick 87. Enhancement of virulence is known as 77. Phagocytic theory was proposed by a. Exaltation b. Attenuation a. Louis Pasteur b. Elie Metchnikoff c. Both a and b d. None of these c. Behring d. Widal 88. The virulence of a pathogen is usually 78. Anaphylaxia was first observed by measured by a. Parter & Richet b. Coombs a. LD b. MLD c. Gell d. None of these c. ID d. All of the above
  18. 18. $ MCQs IN MICROBIOLOGY89. The lethal dose required to kill 50% of the 99. Staphylococcus aureus was isolated by lab animals tested under standard called a. Rosenbach a. ID b. LD50 b. Louis Pasteur c. ID50 d. MLD c. Passet90. The most important virulence factors are d. Sir Alexander Ogston a. Adhesions b. Invasiveness 100. Pseudomonas aeruginosa was first c. Toxigenicity d. Enzymes named e. All of the above a. Schroeter and Gessard91. The ability of a pathogen to spread in ths b. Robert Koch host tissues after establishing the c. Louis Pasteur infection is known as d. Edward Jenner a. Adhesion b. Invasiveness 101. T. pallidum was discovered by c. Toxigenicity d. None of these a. Robert Koch92. Which is the following enzyme acts as a b. Schaudinn and Hoffman spreading factor? c. Louis Pasteur a. Hyaluronidase b. Coagulase d. Edward Jenner c. Catalase d. DNase 102. Neisseria gonorrhoeae was first described93. Vibrio Cholerae was discovered by by a. Koch b. Metchnikoff a. Neisser in 1879 b. Pasteur in 1878 c. John Snow d. Virchow c. Robert Koch d. None of these94. E.coli was first isolated by 103. Rh factor of the blood was discovered by a. Louis Pasteur b. Escherich scientist c. Shiga d. Robert Koch a. Louis Pasteur95. Mycobacterium tuberculosis was first b. Landsteiner and Weiner discovered by c. Janskey a. Robert Koch b. Edward Jenner d. Moss c. Louis Pasteur d. None of these e. None of these96. Mycobacterium lepree was discovered by 104. Trepanema pallidum was discovered by a. Robert Koch b. Hansen a. Schaudinn and Hoffman c. Edward Jenner d. Louis Pasteur b. Louis Pasteur97. Streptococcus pneumoniae was isolated c. Burgey by d. Laennec a. Robert Koch e. None of these b. Edward Jenner 105. Fluroscent substance used in fluorescent c. Antony von Leewenhock microscopy are d. Louis Pasteur a. Quinine sulphate b. Auramine98. B.anthracis was isolated by c. All of these d. None of these a. Louis Pasteur b. Robert Koch c. Antonyvon Leewenhok d. None of these
  19. 19. HISTORY OF MICROBIOLOGY %ANSWERS 1. a 2. a 3. a 4. b 5. b 6. a 7. b 8. d 9. c 10. b 11. b 12. c 13. b 14. d 15. c 16. d 17. b 18. a 19. b 20. b 21. c 22. a 23. c 24. b 25. d 26. c 27. c 28. c 29. b 30. b 31. a 32. b 33. b 34. a 35. c 36. c 37. c 38. a 39. b 40. c 41. d 42. a 43. c 44. b 45. c 46. a 47. b 48. a 49. a 50. d 51. d 52. b 53. b 54. c 55. a 56. c 57. a 58. c 59. a 60. b 61. d 62. c 63. c 64. b 65. b 66. a 67. c 68. a 69. b 70. a 71. a 72. b 73. b 74. b 75. b 76. a 77. b 78. a 79. d 80. a 81. b 82. c 83. a 84. b 85. c 86. b 87. a 88. d 89. b 90. e 91. b 92. a 93. b 94. b 95. a 96. b 97. d 98. b 99. b 100. a 101. b 102. b 103. b 104. a 105. c
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  21. 21. CHAPTER 2 BACTERIA AND GRAM STAINING1. Cold like symptoms are caused by which 7. The functions of plasmid are bacteria a. DNA replication a. Pseudomonas b. Protein synthesis b. E.coli c. Cell wall synthesis c. Haemophilus influenza d. None of the above d. Haemophilus streptococcus 8. Mycoplasmas are bacterial cells that2. In Streptococcus fecalis, the conjugation a. Fail to reproduce on artificial meida takes place at b. Have a rigid cell wall a. Pili b. Cell membrane c. Are resistant to penicillin c. Cell wall d. Flagella d. Stain well with Gram’s stain3. The infected mad dogs may contain 9. The etiologic agent of botulism is a a. Nergi bodies b. Niagri bodies a. Neurotoxin b. Endotoxin c. Negri bodies d. Neisser bodies c. Enterotoxin d. All of the above4. What disease the Nesser will produce? 10. The bacterial cells are at their metabolic a. Mumps b. Rubella peak during c. Polio d. Measles a. Lag phase b. Log5. Rancidity in spoiled foods is due to c. Stationary d. Decline a. Lipolytic organisms 11. Protein particles which can infect are b. Proteolytic organisms called c. Toxigenic microbes a. Virons b. Prions d. Saccharolytic microbes c. Nucleoida d. None of these6. The Baterium that is most commonly used 12. In most of purple bacteria, the light in genetic engineering is harvesting centers are a. Escherichia b. Klebsiella a. B 850 & Fe-S b. B 850 & B 875 c. Proteius d. Serratia c. B 845 & B 875 d. B 850 & B830
  22. 22. MCQs IN MICROBIOLOGY13. Endotoxin produced by gramnegative c. Tetrahymena thermophila bacteria is present in d. Cryptaporiclium a. Peptidoglycan b. Lippolysacharide 22. Which of the following is most similar to c. Theichoic acid d. Inner membrane Rickettsia and Chlamydia?14. Which one of the following was Gram- a. Bdellovibrio b. Clostridium negative, chemolithotrophic bacteria? c. Mycobacterium d. Mycoldaima a. Siderococcus b. E.coli c. Spirellum d. Mycoplasms 23. How would you distinguish pseudomonas species from E-cloi?15. The mode of reproduction which occurs in mycoplasma is a. Gram staining a. Budding b. Bursting b. Morphology c. Binary fission d. Binary fusion c. Glucose fermentation Vs Respiration16. Which one of the following is about d. All of the above Herpes viruses? 24. Which of the following is pathogenic to a. Icosahedral, with envelope, ds DNA humans? b. Polyhedral with envelope, ds DNA a. Spirogyra b. Cephaleuros c. RNA, helical with envelope c. Prototheca d. Both b and c d. ds DNA, brick shape 25. Tumer inducing plasmids are extensively17. Which one of the following produce used in production of typical fried egg appearance colonies on solid media? a. Avirulent phases a. Mycobacteria b. Mycoplasts b. Single cell proteins c. Mycoplasms d. Bacteroides c. Transgenic plants18. An organism that is osmophilic and has d. Nitrogen fixing bacteria a specific requirements for sodium 26. The viruses that live as parasites on bac- chloride resembles teria are a. Halophile b. Basophile a. Fungi b. Commensels c. Barophile d. Xerophile c. Bacteriophages d. None of these19. A population of cells derived from a single cell are called 27. The anthrax disease is most frequently infected from a. Monclonal cells b. Clones c. Protoplasts d. Sub culture a. Cattle b. Sheeps c. Rats d. Both a and b20. Hetrolactic acid bacteria produce a. Lactic acid only 28. The colonies produced by Pseudomonas b. Lactic acid + H2O + CO2 on Mac Conkey’s medium are c. Lactic acid + CO2 a. Purple colored b. Pink colored d. Lactic acid + alchohol + CO2 c. Pale colored d. Green colored21. In which of the follwing microorganism, 29. Staining material of gram positive conjunction tube was not produced bacterium is during conjunction process? a. Fast green b. Haematoxylon a. Thiobaillus thiooxidence c. Crystal violet d. Safranin b. T. ferroxidance
  23. 23. BACTERIA GRAMSTAINING 30. The pigment present in red algae is 39. Erythrocytes will get its ATP energy only a. Rhodochrome by b. Fucoxanthin a. Glycolysis b. Kreb’s cycle c. Chlorophyll only c. Electron Transport d. HMP shunt d. Chlorophyll + phycobilin 40. Virus will contain 31. During mitosis, synapsis occurs in the a. Cell membrane b. Cell wall phase called c. DNA d. DNA or RNA a. Telophase b. Anaphase 41. The bacterial pili mainly contain c. Prophase d. None of the above a. Carbohydrates b. Lipids 32. Which of the following change is a c. Proteins d. Minerals transition? a. ATGC’!ATCC b. ATGC’!ATGG 42. The wonder drug of second world war is c. ATGC’!AGGC d. None of these produced by a. Algae b. Fungi 33. Citrus canker is caused by c. Bacteria d. Plants a. Phytomonas b. Salmonella c. Lactobacillus d. Hay bacillus 43. Role of bacteria in carbon cycle is 34. Bacteria that are responsible for a. Photosynthesis fermentation of dairy milk are b. Chemosynthesis a. Azetobacter b. Rhizobium c. Breakdown of organic compounds c. Lactobacillus d. Hay bacillus d. Assimilation of nitrogen compounds 35. The fungal disease that affect the internal 44. Centromere is that part of chromosome organs and spread through the body are where called a. Nucleoli are formed a. Mycoses b. Systemic mycoses b. Crossing over takes places c. Mycotoxicosis d. Superficial mycoses c. Chromatids are attached 36. The staining technique used to stain the d. Naking occurs metachromatic granules of Corynebacte- 45. Somatic cell of the adult body are haploid rium in many except a. Giemsa stain b. Alberts stain a. Vertebrates b. Invertebrates c. Acid fast staining d. Both a and b c. Fungi d. Vascular plants 37. The orderly increase in all components 46. Congential diseases are of protoplasm of a cell is called a. Diseases present at birth a. Reproduction b. Cell division b. Deficiency disease c. Growth d. All of the above c. Occur during life 38. The causative organism of cholera, i.e., d. Spread from one individual to another Vibrio show the movement called a. Gliding movement 47. The enzyme needed in biological systems for joining two molecules is called b. Darting movement c. Pseudopoidal movement a. Lyases b. Diastases d. None of these c. Polymerases d. Hydrolase
  24. 24. MCQs IN MICROBIOLOGY48. Meosomes are the part of 58. The potorespiration involves a. Plasma membrane b. ER a. Calvin cycle b. Hatch-Slack cycle c. Lysosomes d. Golgi c. Glycolate cycle d. Kreb’s cycle49. All prokaryotes are surrounded by a cell 59. Bioleaching is done by wall except a. Protozoa b. Bacteria a. Mycoplasms b. Sperochetes c. Algae d. All of the above c. Actinomycetes d. Methanogena 60. Inclusion bodies diagnostic of rabies are50. Enzyme hydrolyzing bacterial cell wall called a. Lysozome b. Reductase a. Elementary bodies b. Pascheur bodies c. Protease d. Lysozyme c. Negri bodies d. Guarnieri bodies51. Cows can digest straw because they contain 61. Which of the following genera is most a. Cellulose hydrolyzing microorganisms likely to contain organisms capable of b. Protein hydrolyzing bacteria surviving high temperature? c. Lipid hydrolyzing microorganisms a. Vibrio b. Pseudomonas d. Amino acid degrading bacteria c. Torula d. Coxiella52. The nucleus controls protein synthesis in 62. The major role of minor elements inside the cytoplasm by sending living organisms is to act as a. Chromatin b. A DNA template a. Co-factors of enzymes c. m RNA molecule d. A pecialized protein b. Building blocks of important amino acids53. The site of energy production in a cell c. Constituents of hormones a. Micro body b. Chromosome d. Binder of cell structure c. Ribosome d. Mitochondria 63. The apparatus used to maintain a54. Thylakoid is present in continuous culture a. Mitochondria b. Chloroplast a. Chemostat b. Autostat c. ER d. Golgi apparatus c. Thermostat d. Both a and c55. Which one of the following bacteria has 64. The test used to detect the deamination found extensive use in genetic engineering of the amino acids by bacteria work in plants? a. Nessler’s reagent test a. Clostridum septicum b. Proteolytic test b. Xanthomonas oriza c. Lactose test c. Bacillus coagulens d. Rose aindole reagent test d. Agrobacterium tumefaciens 65. Diphtheria is caused by56. Maximum application of animal cell culture technology today is in the a. Corynebacterium b. Staphylococcus production of c. Streptococcus d. None of these a. Insulin b. Interferons 66. Koplic spots observed in the mucous c. Vaccines d. Edible proteins membrane is characteristic feature of the57. Bacterial ribosomes are composed of disease a. Protein and DNA b. Protein and mRNA a. Rubella b. Measles c. Protein and rRNA d. Protein and tRNA c. Mumps d. Influenza
  25. 25. BACTERIA GRAMSTAINING ! 67. A bacterium containing prophage is c. Frchs bacillus called as d. Koch’s bacillus a. Lytic b. Lysogen 77. Acridine dyes are more effective against c. Lytogen d. None of these a. Gram positive b. Gram negative 68. The most infectious food borne disease is c. Ricke Hsia d. Mycoplasma a. Tetanus b. Dysentery 78. In bacteria pigment bearing structures are c. Gas gangrene d. Botulism a. Chloroplast b. Protoplast 69. An example for common air borne c. Sphaeroplast d. Chromatophores epidemic disease a. Influenza b. Typhoid 79. The procedure of differential staining of bacteria was developed by c. Encephalitis d. Malaria a. A.H. Gram b. H.C. Gram 70. Vrial genome can become integrated into c. N.C. Gram d. H.A. Gram the bacterial genomes are known as a. Prophage b. Temperatephage 80. Intermediate group of pathogen between c. Bacteriophage d. Metaphage bacteria and viruses which are intracellular parasites are called 71. Rancidity of stored foods is due to the a. Mucoplasmas b. Rickettsias activity of c. Prions d. Virusoides a. Toxigenic microbes b. Proteolytic microbes 81. Bacillus is an example of c. Saccharolytic microbes a. Gram positive bacteria d. Lipolytic microbes b. Gram negative bacteria 72. Virion means c. Virus d. Viroid a. Infectious virus particles b. Non-infectious particles 82. Amoebic dysentery in humans is caused c. Incomplete particles by d. Defective virus particles a. Plasmodium 73. Virulence of the microorganisms can be b. Paramecium reduced by c. Yeast a. Attenuation b. A virulence d. Entamoeba histolytica c. Inactivation d. Freezing 83. Viral genome that can become integrated into bacterial genome is called 74. The test used for detection of typhoid fever a. WIDAL test b. ELISA a. Prophage b. Temperate phage c. Rosewaller test d. Westernblotting c. Bacteriophage d. Metaphage 75. Bacteriophage capable of only lytic 84. Cytochromes are growth is called a. Oxygen acceptors b. ATP acceptors a. Temperate b. Avirulent c. Electron acceptors d. Protein acceptors c. Virulent d. None of these 85. The cells having F plasmid in the 76. Diphtheria bacillus is otherwise known as chromosomes were termed as a. Fried-Landers bacillus a. Hfr b. F– + b. Kleb’s hofflers bacillus c. Hbr d. C
  26. 26. MCQs IN MICROBIOLOGY86. Recombination process occurring through 97. Pigment bearing structure of bacteria are the mediation of phages is a. Mesosomes b. Plasmids a. Conjunction b. Transduction c. Mitochondria d. Chromophores c. Transformation d. Transfection 98. Spirochete is87. Mordant used in grams staining is a. Gonococci a. Crystal violet b. Iodine b. Strphylococci c. Saffranin d. All of these c. Treponema pallidum88. Parasitic form must contain d. Streptococci a. Capsule b. Cell-wall 99. Histones are found in c. Endospores d. Flagella a. Prokaryotes b. Eukaryotes89. Gram staining is an example for c. Viruses d. None of these a. Simple staining b. Differential staining 100. Cell wall of gram negative bacteria is c. Negative staining d. None of these a. Thick90. Following Cocci are non-motile except b. Lipids are present a. Staphylococcus b. Meningococcus c. Teichoic acids are absent c. Gonococcus d. Rhodococcus agilis d. None of these91. Aspergillus fumigatus can infect 101. Cytoplasmic streaming is present in a. Birds b. Animals a. Prokaryotes b. Animals c. Man d. All of them c. Eukaryotes d. Both a and b92. Enterotoxin responsible for food poisoning 102. The motile bacteria is is secreted by a. S. typhi b. K. pneumoniae a. Enterococci b. Entamoeba histolytica c. Enterobacteriaceae d. Straphylococci c. B. anthracis d. Shigella93. Autolysis is done by 103. The stain used to demonstrate fungus a. Mitochondria b. Lysosomes a. Albert c. Golgi bodies d. Peroxisomes b. Nigerosin c. Lactophenol cotton blue94. A facultative anaerobic is d. None of these a. Only grow anaerobically b. Only grow in the presence of O2 104. Exotoxina are c. Ordinarily an anaerobe but can grow with a. Heat labile O2 b. Heat stable d. Ordinarily an aerobe but can grow in c. Part of cell wall absence of O2 d. Polymerized complexes95. The percentage of O 2 required by 105. The viruses that attack bacteria are moderate anaerobe is a. Bacterial viruses b. Bacterial pathogens a. 0% b. 0.5% c. 2 – 8% d. 5 – 10% c. Bacteriophages d. Various96. Interferon is formed by 106. The size of virus particle may range a. Lymphocytes b. Lymphoblasts a. 0.02–0.2 ìm b. 0.5–10 ìm c. Fibroblasts d. All of these c. 0.015–0.2 ìm d. 0.1–100 ìm
  27. 27. BACTERIA GRAMSTAINING #107. The bacterial cell multiplication is usually 117. The differences between Gram positive by and Gram negative bacteria is shown to reside in the a. Mitosis b. Meiosis c. Conjugation d. Binary-fission a. Cell wall b. Nucleus c. Cell membrane d. Mesosomes108. Rod shaped bacteria are known as 118. Capsule formation occurs in the presence a. Cocci b. Comma forms of c. Bacilli d. Plemorphic froms a. Albumin b. Charcoal109. All the groups of bacteria have cell wall c. Serum d. Starch a. Mycobacteria b. Mycoplasmas 119. The virulence determining antigens of c. Clostridia d. Rickettsia microorganisms may be110. Thickness of cell wall ranges from a. Proteins and polysaccharides a. 9-10 nm b. 12-13 nm b. Carbohydrate – protein complexes c. 10-25 nm d. 30-40 nm c. Polysaccharide – Phospholipid – Protein complexes111. Teichoic acids and Teichuronic acids are d. All of these found in a. Gram positive bacteria 120. Organelles with hydrolytic enzymes are b. Gram negative bacteria a. Mitochondria b. Golgi complex c. Fungi c. Lysosomes d. Ribosomes d. None of these 121. Bacterial locomotion is accomplished by112. Meosomes are a. Fimbria b. Flagella a. Kind of ribosomes c. Cytoskeleton d. Both a and b b. Formed during cell lysis 122. Fimbriae are demonstrated by c. A part of cell wall a. Culture d. Principal sites of respiratory enzymes b. Gram stain113. The characteristic shape of the bacteria c. Biochemical reactions is maintained because of d. Haemaggulation test a. Capsule b. Cell wall 123. The motile bacteria is c. Cell membrane d. Slime layer a. Salmonella typhi114. Bacterial capsule is chemically composed b. Klebsiella pneumoniae of c. Bacillus anthracis a. Polypeptide d. Shigella flexneri b. Polynucleotides 124. Following cocci are non-motile except c. Polysaccharides a. Staphylococcus b. Meningococcus d. Polypeptides or polysaccharides c. Gonococcus d. Rhodococcus agilis115. The cell wall deficient form of bacteria is 125. Metachromatic granules are chemically a. Mycoplasma b. ‘L’ form composed of c. Protoplast d. Spheroplast a. Lipids116. Mesosomes are also known as b. Proteins c. Polymetaphosphates a. Mitochondria b. Chloroplasts d. Polysaccharide c. Golgi complex d. Chondroids
  28. 28. $ MCQs IN MICROBIOLOGY126. Metachromatic granules can be stained 135. The action of alcohol during Gram- with staining is a. Saffranine b. Methylene blue a. Allows the color c. Crystal violet d. Pienic acie b. It adds color c. Decolorises the cells127. Bacteria multiply by d. None of these a. Spore formation b. Simple binary fission c. Conjugation d. Gametes 136. Lipid contents is more in a. Gram negative bacteria128. Bacterial spores are b. Gram positive bacteria a. Weakly acid fast b. Strongly acid fast c. Same in both c. Alcohol fast d. Non acid fast d. None of these129. Endospores can be stained with 137. Cell-wall is a. Safranine b. Crystal violet a. Thick in Gram positive than Gram negative c. Methylene blue d. Malachite green b. Thick in Gram negative than Gram positive130. The following bacteria produce pigment, c. Equal in both except d. In Gram negative cell-wall is absent a. Pseudomonas pyocyaneus 138. The Lipid content present in Gram positive b. Serratia marcescens bacterial cell-wall is c. D. pneumoniae a. 1-10 % b. 1-5 % d. Staphylococcus aureus c. 2-8 % d. None of these131. The order of stains in Gram-staining 139. Rickettsiae stained by this technique procedure is responds as a. Crystal violet, Iodine solution, Alcohol, a. Gram positive Saffranine b. Gram negative b. Iodine solution, Crystal Violet, Saffranine, c. Between positive and negative Alcohol d. None of these c. Alcohol, Crystal Violet, Iodine solution, 140. Chlamydiae occur in Saffranine d. All of these a. Elementary bodies b. Reticulate bodies c. Complex structures d. a and b132. The percentage of alcohol used in Gram- staining is 141. Chlamydiae can be stained better with a. 75% b. 90% a. Ziehl neelsen staining c. 60% d. 25% b. Castaneda Machiavello stains c. Giminez stains133. Gram positive bacteria appear as d. Both b and c a. Pink b. Violet 142. Algae means c. both a b d. None of these a. Fresh water organisms134. Gram negative bacteria appear as b. Sea weeds a. Pink b. Violet c. Fresh water weeds c. both a b d. None of these d. None of these
  29. 29. BACTERIA GRAMSTAINING %143. The study of algae is known as 154. The extra cellular infections virus particle a. Algalogy b. Phycology is called c. Mycology d. Bacteriology a. Capsid b. Nucleocapsid c. Virion d. None of these144. The free floating algae are known as 155. Shape of bacteriophage is a. Phytoplankins b. Benthons c. Sea weeds d. None of these a. Brick shape b. Bullet shape c. Helical shape d. Tadpole shape145. Sexual reproduction of algae is carried by 156. If only one stain is used for staining a a. Isogamy b. Anisogamy specimen c. Oogamy d. All the above a. Simple staining b. Negative staining146. In algae, advanced type of sexual repro- c. Differential staining d. None of these duction is 157. Other than the sample (specimen) the a. Isogamy b. Anisogamy remaining portion is stained then it is called c. Oogamy d. None of these a. Simple staining b. Negative staining147. Alginic acids and its salts are obtained c. Differential staining d. None of these from the wall of 158. If more than one stain is used, such a. Red algae b. Brown algae staining is called c. Green algae d. Red and brown algae a. Simple staining b. Negative staining148. The molds obtained nutrition from dead c. Differential staining d. None of these and decaying matter which are called 159. ‘Fluorescence’ was first observed by a. Saphrophytes b. Parasites a. Kohler b. Coons c. Commensals d. None of these c. Both a and b d. None of these149. Most molds are capable of growing in 160. By using fluorescence property fluorescent the temperature range between antibody technique was developed by a. 0o – 25oC b. 0o – 35oC a. Kohler b. Coons c. 10o – 25oC d. 10o – 35oC c. Both and b d. None of these150. Examples for actinomycetes 161. During staining for Electron Microscopy, a. Streptomyces b. Spirillospora the method which improves contrast of specimen is c. Frankia d. Dermatophillia e. All of the above a. Positive staining b. Negative staining c. Shadow staining d. None of these151. Pellicle is found in only 162. The inorganic forms of nitrogen, which a. Algae b. Fungi are accepted by bacteria are c. Bacteria d. Protozoans a. Nitrates b. Nitrites152. The Largest virus is c. Ammonium salts d. All of these a. Parvo virus b. Pox virus 163. Archaeo bacteria are known as c. Rhabdo virus d. None of these a. Halophiles153. The smallest virus is b. Red extreme halophiles a. Parvo virus b. Rhabdo virus c. Osmophiles c. Pox virus d. Adeno virus d. Extreme thermophiles
  30. 30. MCQs IN MICROBIOLOGY164. Nitrite is converted into nitrate by the 173. Phycobiont is bacteria a. The algal part in Lichens a. Nitrosomonas b. Nitrosocytes b. The fungal part in Lichens c. Nitrobacter d. Azatobacter c. Laustoria formation d. None of these165. Sulphur oxidizing bacteria is a. Alcaligenes b. Pseudomonas 174. Parasitic form must contain c. Thiobacillus d. None of these a. Capsules b. Cell-wall c. Endospores d. Flagella166. Bacillus Schlegelli is 175. The total no. of genes in the group of a. Hydrogen – Oxydising bacteria same individuals is b. Sulphur – Oxydising bacteria a. Genome b. Gene map c. Iron-Oxidising bacteria c. Gene pool d. None of these d. Nitrite oxidizing bacteria 176. Transformation was observed mainly in167. The group of bacteria which deopends on a. Bacteriophages b. Temperate phages organic sources in nature for their energy c. λ –phage d. All of these requirements. They are said to be a. Chemotrophs b. Phototrophs 177. Capsulated forms of bacteria are c. Heterotrophes d. Organotrophs a. Virulent b. A virulent c. Useful d. Symbiotic168. Majority of bacteria are 178. The bacterial cells participating in a. Saprophytes b. Symbionts conjugation are c. Commensals d. Parasites a. Conjugants b. Fertile cells169. Symbionts are c. Exconjugants d. None of these a. Bacteria in symbiotic association 179. Phagocytes are b. The group of fungi in symbiotic association a. Monocytes b. Macrophages c. The groups participating in symbiotic c. Basophils d. All of these association d. All of these 180. The microorganism engulfed by phago- cyte resides in a vacuole is known as170. The best example for symbiotic associa- a. Phagosome b. Lysosome tion is c. both a and b d. None of these a. E.coli in intestine of man b. Lichens 181. Toxic products in phagolysosome are c. Normal floraof skin a. H2SO4 d. All of the above b. Singlet O2 c. Superoxide radicals171. The enzymes responsible for decomposi- d. All of these tion is a. Lipolytic b. Proteolytic 182. During destruction of antigen particle in phagolysosome the product formed in c. Lysozyme d. Both a and b phagolysosome the product formed172. Urea is decomposed by the species during formulation is a. Micrococcus sps. b. Nitrosomonas sps. a. Acetic acid b. Lactic acid c. Proteus sps. d. Both a and c c. Citric acid d. None of these
  31. 31. BACTERIA GRAMSTAINING 183. The coating of a bacterium with antibody 192. Vibrio cholera differs from vibrio eltor by or complement that leads to enhanced a. It shares some Inaba, Ogawa subtypes with eltor phagocytosis of the bacterium by b. Resistant to polymuxin phagocytes is called c. Eltor is non-motile a. Opsonisation b. Aggulation d. Causes less subclinical infections as compared c. CFT d. None of these to eltor184. Attenuation means 193. Cholera vaccine gives protection for a. Killing of the bacteria (microorganism) a. 1 – 3 months b. 3 – 6 months b. Inactivation of bacteria c. 6 – 9 months d. 9-12 months c. More activating the bacteria 194. Prophylaxis of cholera is d. Both 1 and 2 a. Protected water supply185. Infection that results in pus formation are b. Environmental sanitation called c. Immunisation with killed vaccines a. Focal infection b. Acute infection d. All of these c. Pyogenic infection d. Chronic infection 195. Sh.dysenteriae is also known as186. Presence of viable bacteria in the blood stream is called a. Sh.shiga b. Sh.schmitzi a. Viraemia b. Septicaemia c. Both a and b c. Bacteraemia d. Bactericidal d. Sh.para dysenteriae187. Presence of viruses in the blood stream is known as 196. Acid fast bacteria are a. Viraemia b. Bacteraemia a. Neisseria b. Staphylococci c. Septicaemia d. Pyemia c. Mycobacteria d. All of the above188. Opsonin is the 197. Mycobacteria are stained with a. Cellwall component a. Gram’s staining b. Plasma component b. Simple staining c. Serum component c. Both a and b d. Cytoplasm component d. Ziehl – Neelsen’s staining189. β-haemolytic bacteria is 198. Niacin test is positive in case of a. Streptococcus pyogenes a. Corynebacterium b. Str. pneumoniae b. M. tuberculosis c. Str. viridans c. M. bovis d. Str. faecalis d. M. avium190. The natural reservoir of infection for 199. Lepromin test cholera is a. Is negative in tubercular leprosy a. Flies b. Horse b. Positive in lepromatous type c. Man d. None of these c. Indicated delayed hypersensitivity test191. Main cause for Cholera is d. Indicates infection a. Poverty and insanitation 200. Streptococcus forms causes which type of b. Mosquitoes infections? c. Toxin produced by pesticides a. Fever b. Zoonotic d. None of these c. Pyogenic d. None of these
  32. 32. MCQs IN MICROBIOLOGY201. Streptococcus pyogenes classification is 211. Mc Fadyean’s reaction is used to detect based on a. Bacillus anthracis b. Brucella a. Protein M b. Protein T c. Corynaebacterium d. None of these c. Protein R d. Polysaccharide C 212. Gasgangarene bacillus is202. α-haemolytic streptococci are also known a. Facultative anaerobe as b. Obligate anaerobe a. Str. pyogenes b. Virulence group c. Facultative aerobe c. Viridans group d. None of these d. Obligate aerobe203. Streptolysin O is inactivated by 213. Coagulase test is used for a. CO2 b. Nitrogen a. Salmonella c. Oxygen d. Serum b. Staphylococcus204. Streptolysin ‘S’ is c. Bordetella a. Oxygen unstable b. Thermostable d. Pneumococcus c. Oxygen stable d. None of these 214. HIV is belonging to205. Influenza virus is identified by using a. Retro Viridae b. Rhabdo Viridae a. Haemaggulutinin inhibition test c. Toga Viridae b. Tissue culture method d. Paramyxo Viridae c. Embryonated eggs d. Plaque formation 215. Special feature of Retro viruses206. Growth of influenza virus is identified by a. Reverse transcriptase b. RNA directed DNA polymerases a. Cytopathic effects b. Hela cells c. Both a b c. Both a and b d. None of these d. Boils207. Glutamic acid is oxidized by the species 216. AIDS virus is except a. RNA virus b. DNA virus a. B. abortus b. B. melienasis c. Retro virus d. Entero virus c. B. suis d. B.canis 217. AIDS is caused by208. “Prozone phenomenon” is encountered in a. HTLV – I b. Bunya virus a. A typical mycobacteria c. HTLV – III d. All b. Brucella 218. Which of the following organisms is most c. Streptococcus commonly associated with AIDS pneumo- d. Bordetella pertusis nia? a. Klebsiella209. Of the following, this is a capsulated b. Str. pneumonia organism c. Mycoplasma a. Bacillus anthracis b. Escherichia-coli d. Mycobacterium tuberculosis c. Corynebacterium d. Brucella 219. Sero conservation in HIV infection takes210. Anthrax is a place in a. Vector borne b. Zoonotic infection a. 3 weeks b. 6 weeks c. Wound bone d. Soil borne c. 9 weeks d. 12 weeks
  33. 33. BACTERIA GRAMSTAINING 220. Following is the marker of HIV infection 230. Diagnosis of bacterial disease can be in blood: made by a. Reverse transcriptase a. Finding bacteria in pathological fluids b. DNA polymerase b. Isolation of bacteria by culture from exudates c. RNA polymerase or blood d. None of these c. Both a and b d. None of these221. Which of the following is the most specific in diagnosis of AIDS? 231. Staphylococcus aureus are characterized by a. IHA b. Western blot a. Formation of acid in sucrose, dextrose c. ELISA b. Liquification of gelatin due to production of gelatinase d. Immuno electrophoresis c. Strains are catalase positive222. The interval period between HIV infection d. All of above and appearance of antibodies in serum e. None of these is called 232. Cholera occurs in _______ form a. Intrinsic period b. Incubation period c. Window period d. None of these a. Endemic b. Epidemic c. Sporadic d. all223. Screening test for AIDS is e. None of these a. Western blot test b. ELISA test 233. Endemic typhus is caused by c. Both a and b d. VDRL test a. R.mooseri b. R.quintana224. Confirmatory test for AIDS is c. R.prowazekii d. any of them a. Western blot test b. ELISA test e. None of these c. Karpas test d. Fujerbio test 234. A man is usually infected for tick typhus225. The most common infection in AIDS is by – a. LGV b. CMV a. Drinking milk of sick animals c. Pnemocystis carnii d. Syphilis b. Tending cattle c. Inhaling infected dust226. During AIDS, HIV infects d. All of these a. CD3 lymphocytes b. CD4 lymphocytes c. CD2 lymphocytes d. Blymphocytes 235. In Gram positive bacteria, ratio of RNA to DNA is227. Lab diagnosis of Leishmaniasis is done by a. 8 : 1 b. 1 : 2 a. CFT b. Peripheral smear c. Almost equal d. None of these c. Blood culture d. All of these 236. Ziehl – Neelson stain is a ______228. Those fungi which do not have a sexual a. Simple stain b. Counter stain stage are classified as c. Differential stain d. None of them a. Phycomycetes b. Ascomycetes c. Basidiomycetes d. Fungi imperfecti 237. Wet mount slide preparations are used in microbiology as they allow to see229. Tinea capitis is a. Size and shape of individual organisms a. Ring worm of the foot b. Characteristic arrangement or grouping of cells b. Ring worm of scalp c. Motility of the organism c. Ring worm of non-hairy skin of body d. All of these d. Both a and c e. None of these
  34. 34. MCQs IN MICROBIOLOGY238. Organism resistant to degradative b. Invasion of mucous membrane lysosomal enzymes includes c. Contamination of wounds with conidia or a. M.tuberculosis myceliat fragments b. Legionella pneumophila d. All of these c. M.leprae e. None of these d. Both a and b 245. Fungi differs with bacteria in that it – e. Both b and c a. Contain no peptidoglycan239. Freeze-etch particles (used in preparing b. Are prokaryotic cell for electron microscopy) can be c. Susceptible to griseofulvin located in the d. Have nuclear membranes a. Cytoplasm b. Cell wall e. All of these c. Cell membrane d. Nucleus 246. A polysaccharide capsule is present on240. The properties common to Gram positive cryptococci which – and negative cell walls are a. Inhibits phagocytosis a. Equal susceptibility to hydrolysis by lysozyme b. Is an aid to diagnose b. Peptide crosslinks between polysaccharides c. Cross reacts with rheumatoid factor c. Rigid peptoglycon activity d. All of these d. Greater resistance to drying than vegetative cell. 247. The largest protozoa is – e. All of these a. Balantidium coli b. Entamoeba coli241. The main difference in true bacteria and c. Trichomonus vaginalis mycoplasma is that it does not posses – d. Toxoplasma gondii a. Flagella b. Cell wall c. ATP synthesis d. A capsule 248. Premunition is particularly seen in – a. Ascaris b. Giardia242. The organism responsible for retarding penetration of host cell by an inhibitor of c. Plasmodium d. None of these ATP synthesis. 249. Which of the following vaccine contains a. M.pneumoniae attenuated form of bacteria? b. Rickettsia rickettsii a. BCG b. TAB c. Chlamydia trachomatis c. Polio d. Cholera d. Chlamydia psitacci 250. The bacteria, which is motile at 22oC but243. Mycoplasmas differ from Chalamy-diae non-motile at 37oC is in that, it a. Tranformation b. Transduction a. has ability to cause urinary tract infection c. Conjugation d. Cell fusion b. lack of atrue bacterial cell wall 251. Techoic acid is – c. susceptible to penicillin a. Found in the walls of Gram positive bacteria d. All of these b. Provide receptors for phages e. None of these c. Make up outer wall of Gram negative244. Fungal disease in human is caused by – bacteria a. Inhalation of conidia d. Influence the permeability of the membrane
  35. 35. BACTERIA GRAMSTAINING !252. One flagelium at one end of the organ is 261. Cholera red reaction is identified by called – a. Sulphuric acid b. Nitric acid a. Monotrichate b. Amphitrichate c. Hydrochloric acid d. Carbolic acid c. Iophotrichate d. Peritrichate 262. Diagnosis of carrier of salmonella typhi253. What is the function of bacterial capsule? may be shown by a. Production of organism from phagocytosis a. Fecal culture b. Bile culture b. Helps in adherence of bacteria to surface in c. Urine culture d. All of these its environment c. Both a and b 263. Daisy head colony is associated with d. None of these a. M.tuberculosis b. C.diphtheriae254. Which of the following is the charachter- c. Cl. tetani d. None of these istic of bacterial spore? 264. Neil mooseri reaction is related to a. Highly refractile a. Rickettsiae b. Chlamydiae b. Usually dehydrated c. Spirochaetes d. C l o s t r i d i u m c. Sensitive to formaldehyde periringens d. All of these 265. All of the following are DNA viruses255. Which of the following are acid fast except – structures? a. Parvo virus b. Paramyxo virus a. Mycobacteria b. Bacterial spores c. Herpes virus d. Pix virus c. Nocardia d. All of these 266. The dengue fever virus is –256. All of the following are acid fast structures except a. Arbo virus b. Echo virus c. Entero virus d. Orthomyxo virus a. Clostridium b. Bacterium spores c. Exoskeleton d. None of these 267. Dengue fever is caused by –257. All of the following are energy source of a. Bacteria b. Virus bacteria except c. Fungi d. Rickettsia a. Oxidation of inorganic compounds 268. Which of the following characters are b. Oxidation of organic compounds related to viruses? c. Absorption of heat a. No growth on inanimate culture media d. Utilisation of visible light b. Not sensitive to antibiotics258. Identify the obligate anaerobes c. No energy producing enzymes a. Salmonella b. Vibrio cholera d. Insensitive to interferon c. Cl. tetani d. Sarcinae 269. Main causative organism of chiken pox is259. Streptococci which are destroyed at 60°C a. Fox virus b. Mumps virus for 30 minutes c. Measles virus d. None of these a. Preptostreptococci b. Strepto viridans c. Strepto hemolyticus d. All of these 270. Rickesia are stained with a. Giesna and Castaneda stains260. Toxins or enzymes which are not produced by streptococcus pyrogens b. Macchiavello and Gimnezstains c. Both a and b a. Hyaluronides b. Phosphate c. Hemolysin d. Streptokinase d. Malachite green
  36. 36. MCQs IN MICROBIOLOGYANSWERS 1. c 2. c 3. c 4. d 5. a 6. a 7. d 8. c 9. a 10. b 11. b 12. b 13. b 14. b 15. c 16. a 17. c 18. a 19. b 20. d 21. a 22. c 23. c 24. c 25. c 26. c 27. d 28. c 29. c 30. d 31. c 32. d 33. a 34. c 35. b 36. b 37. c 38. b 39. a 40. d 41. c 42. b 43. c 44. c 45. c 46. a 47. c 48. a 49. a 50. d 51. a 52. c 53. d 54. b 55. d 56. c 57. c 58. c 59. b 60. c 61. c 62. a 63. a 64. a 65. a 66. c 67. b 68. d 69. a 70. b 71. d 72. c 73. a 74. a 75. a 76. b 77. a 78. d 79. b 80. b 81. a 82. d 83. a 84. c 85. a 86. b 87. b 88. b 89. d 90. a 91. b 92. d 93. b 94. d 95. c 96. d 97. d 98. c 99. b 100. c 101. c 102. a 103. c 104. a 105. c 106. c 107. d 108. c 109. b 110. c 111. a 112. d 113. b 114. d 115. b 116. d 117. a 118. c 119. d 120. c 121. d 122. d 123. a 124. d 125. c 126. b 127. b 128. a 129. d 130. c 131. a 132. b 133. b 134. a 135. c 136. a 137. a 138. b 139. b 140. d 141. d 142. b 143. b 144. a 145. d 146. c 147. b 148. a 149. b 150. d 151. d 152. b 153. b 154. c 155. d 156. a 157. b 158. c 159. a 160. b 161. b 162. d 163. b 164. c 165. c 166. a 167. c 168. d 169. c 170. b 171. b 172. d 173. a 174. a 175. c 176. b 177. c 178. a 179. d 180. a 181. d 182. b 183. a 184. b 185. c 186. c 187. a 188. c 189. a 190. c 191. a 192. d 193. b 194. d 195. c 196. c 197. d 198. b 199. c 200. d 201. a 202. c 203. c 204. c 205. a 206. b 207. d 208. b 209. a 210. b 211. a 212. b 213. b 214. a 215. c 216. c 217. d 218. d 219. c 220. a 221. b 222. c 223. b 224. a 225. c 226. b 227. d 228. d 229. c 230. c 231. c 232. d 233. a 234. c 235. a 236. c 237. d 238. e 239. c 240. d 241. b 242. b 243. b 244. d 245. e 246. a 247. a 248. c 249. a 250. d 251. a 252. a 253. c 254. d 255. d 256. a 257. c 258. c 259. d 260. b 261. a 262. d 263. b 264. a 265. b 266. a 267. b 268. d 269. d 270. c
  37. 37. CHAPTER 3 STERILISATION, CULTURE MEDIA AND PURE CULTURE TECHNIQUES1. The medium used in membrane filter 7. Thermal death time is technique was a. Time required to kill all cells at a given a. EMB agar b. EMR-Vp medium temperature c. Lactose broth d. Endo agar b. Temperature that kills all cells in a given time c. Time and temperature needed to kill all cells2. Lysol is a d. All of the above a. Sterilent b. Disinfectant c. Antiseptic d. Antifungal agent 8. A culture medium the exact composition of which is not known was called as3. Which of the following is a neutral stain? a. Simple b. Complex a. Picric acid b. Gmiemsa c. Defined d. Natural c. Neutral red d. Malachite green 9. Elek’s gel diffusion test is used for the4. Peptone water medium is an example for detection of a. Synthetic medium a. Tetani toxin b. Cholera toxin b. Semisynthetic medium c. Diophtheria toxin d. Toxoid c. Differential medium 10. Temperature required for pasteurization d. None of these is5. The method in which the cells are frozen a. Above 150oC b. Below 100oC dehydrated is called c. 110oC d. None of these a. Pasteurization b. Dessication 11. Separation of a single bacterial colony is c. Disinfection d. Lypophilization calle6. The technique used to avoid all a. Isolation b. Separation microorganisms is accomplished by c. Pure culturing d. All of these a. Sterlization 12. Which of the following is ionizing b. Disinfection radiation? c. Surgical sterilization a. U.V. rays b. IR d. Disinfection Sterilization c. γ-rays d. None of these