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Biotech ppt(final) Biotech ppt(final) Presentation Transcript

  • Title: Testing for natural herbicides, Eliminating the weeds School: Pasir Ris Secondary School Group Member: - Fabian Ho - Myra Koh -Amelia Jane -Lee Hui Xin -Benson Quah Bio-Technology Project 2009
  • Contents
    • Researches
    • How the idea came about
    • Objectives of the project
    • Materials used
    • Information of Weeds
    • Procedures of Experiment
    • Results
    • Pictures - results
    • Discussions
    • Applications
    • Conclusion
    • Miscellaneous
  • Research
    • The main components of commercial herbicides, Phenoxyacid and benzoic acid-derived herbicides are currently used in more than 1,500 different commercial formulations , others include 2,4-dichlorophenoxyacetic acid (2,4-D)
    Fig.2 chemical structure of the 2,4-D Fig.1 3d view of the chemical structure 2,4-D
  • Research
    • Carbon dioxide on the rise, Pollution is affecting the whole world
    • Global Warming may occur very soon
    • We must do something to help as the People of the world
    • As the weeds compete nutrient with the plant, the plant will not grow well and could not minimize the amount of carbon dioxide in the air
  • How the idea came about
    • Idea derived after reading numerous environmental reports
    • Pollution was on the rise
    • A rapid increase in the earth’s temperature, resulting in global warming
    • Chemicals in the herbicides have been proven to be non-environmentally friendly by pollution of water bodies
    • Besides harming the environment, users have also noted to develop an increased risk of cancers, including lymphoma , leukemia , and prostate cancer.
    • Hence, the idea was to find a safer alternative that is both user and environmentally friendly.
    • To find a natural herbicide that doesn’t harms the environment using household products
    • Replace current commercial herbicide that used 2-4, D
    • To find if the absence of water will lead to the death of the weeds
    Objectives of project
    • 15 Chemicals :
      • Boiled Water
      • Half-Boiled Egg
      • Water
      • Coke
      • Rootbeer
      • Walnut
      • Cornflakes
      • Distilled White Vinegar
      • Clove oil
      • Citrus oil
      • Lime
      • Bleach
      • Salt
      • Darlie Toothpaste
      • Soap
    Materials used
    • 100 ml of Measuring cylinder
    • 200 ml of beaker
    • Filter funnel
    • Filter Paper
    • Soil
    • Electronic Balance
    • pH-Meter
    • Lighter
    • Styrofoam Cups
    • Weeds – Phyllanthus Urinaria L
  • Information of weeds
    • Name of weed : Phyllanthus urinaria L
    • Common name : Chanca piedra, shatterstone
    • Overview of weed :
    • A small tropical annual herb growing to 2 feet tall. Along the erect, red stem equally set small green, oblong feathered leaves.
    • Greenish white flowers; small wart-like fruit which is greenish-red, underneath feathered leaves.
    • When the plant is picked the feathery leaves fold in, completely closing themselves.
    • Common weed in Suriname and closely related in appearance and phytochemical structure to two other weeds
  • Procedures
    • Collect weeds ( Phyllanthus Urinaria L ) from eco-garden
    • Put in cups of 15 ;adding mixed burnt soil of 100g into each cups
    • Measure pH of the soil and Chemicals
    • Add in one chemical into one cup, each chemical 10g
    • Repeat step 1-3 for other chemicals
    • Write down the observation before putting it for three days
    • Leave it for 3 Days
    • Write down the observation after three days
    • Repeat the experiment for four times
    • Take the average of the results
    • pH Values :
      • Distilled white vinegar - 2.86
      • citrus oil - 4.36
      • salt solution – 7.00
      • coke - 2.54
      • lime - 2.66
      • rootbeer - 4.75
      • clove oil – 6.12
      • half-boiled egg – 7.90
      • boiled water - 7.00
      • tap water - 7.00
      • soap - 6.74
      • toothpaste solution - 6.84
      • bleach solution - 9.76
      • walnut - 6.31
      • cornflakes - 5.55
    Procedures
  • Results substances Initial length of weeds End length of weeds pH of soil(acidic /alkaline) Did the plant die? Half boiled egg 5.0 cm 4.5 cm 7.90 No Clove oil 5.7 cm 4.9 cm 6.12 Yes Citrus oil 5.5 cm 6.5 cm 4.36 Yes Cornflakes 6.5 cm 4.9 cm 5.55 No Walnuts 5.5 cm 5.7 cm 6.31 No Toothpaste 5.1 cm 6.1 cm 6.84 Yes lime 4.9 cm 3.2 cm 2.66 No Coke 4.9 cm 4.9 cm 2.54 No Boiled water 6.2 cm 6.7 cm 7.00 No Tap water 5.5 cm 3.9 cm 7.00 No Salt solution 4.5 cm 5.0 cm 7.00 Yes Bleach 4.5 cm 5.6 cm 9.76 Yes Rootbeer 4.9 cm 3.2 cm 4.75 No Distilled White Vinegar 5.2 cm 5.5 cm 2.86 Yes Lemon flavored soap 4.5 cm 4.7 cm 6.74 Yes
  • Results
    • Most weeds that have acidic chemicals poured in to the cup of soil are dead.
    • Alkaline chemicals like bleach, toothpaste and soap also causes death to the weeds.
    • Clove oil kills the weeds fastest; ≤2Days.
    • Water cannot be absent for the growth of the plants.
  • Pictures Results Pic 1: Salt Pic 2: Boiled Water Pic 3: Lime Pic 4: Distilled White Vinegar Pic 5: Coke Pic 6: Cornflakes Pic 7: Tap Water Pic 8: Rootbeer Pic 9: Bleach Pic 10: Soap Pic 11: Clove Oil Pic 12: Walnut Pic 13: Citrus Oil Pic 14: Toothpaste Pic 15: Half-Boiled Egg
  • Discussion
    • Chemical can be separate into two groups, Anti-Growth and Promote Growth
    • Half boiled egg ;citrus oil, although killed weeds several days later ,also attracting increasingly large amount of pests
    • Level of acidity also affect weeds growth
    • Black walnut produce a substance called juglone (5 hydroxy-1, 4-napthoquinone)
    • Juglone kills and reduces plant growth like those found under the tree canopies.
    • lack of water was the essential cause for the plant to die in the third experiment
    • Salt is a dehydrating agent so cause death of plant through osmosis
    • In future, the world would be cleaner
    • Families can take into consideration of using household item to kill weeds
    • Less Pollution Occur
    • More Environmental friendly
    • Chance of global warming decrease
    • Safer environment to live in
  • Conclusion
    • Most of the acidic chemicals used in the experiment is suitable to be an alternative for the current commercial herbicide.
    • Alkaline substance are also natural herbicides
    • Environmental-friendly herbicides that are made from household items are used widely, it would decrease the use of commercial herbicides.
    • Thus, it will decrease the negative impact on the environment caused by chemical 2,4-D .
  • Miscellaneous
    • http://www.24d.org/Presentations2/Chronology-expert-reviews-August-2007.pdf
    • Http://www.epa.gov/fedrgstr/EPA-PEST/2007/August/Day-08/p15109.htm
    • http://www.24d.org/Presentations2/Value-Benefit-Presentation-Webpage.pdf
    • http://www.24d.org/Presentations2/Landscape-Alberta-Update-March-2008.pdf
    • http://www.osha.gov/SLTC/healthguidelines/ethanolamine/recognition.html.
    • http://davesgarden.com/guides/articles/view/2187/
    • http://science.jrank.org/pages/3187/Halide-Organic-Organofluorides.html
    • http://www.moscowfood.coop/archive/VinegarKillsWeeds.html
    • http://www.coopext.colostate.edu/4DMG/Weed/bleach.htm
    • http://asap.sustainability.uiuc.edu/org-ag/weeds/cover-thistle
    • http://www.postandcourier.com/news/2008/jul/20/organic_options48035/
    • http://www.finegardening.com/how-to/articles/vinegar-based-citrus-based-weed-killers.aspx
    • http://organicgardens.suite101.com/article.cfm/natural_herbicides_are_safe_and_inexpensive
    • http://encyclopedia.stateuniversity.com/pages/9965/herbicide.html
    • http://www.herbi-systems.com/FAQ_s/faq_s.htm
    • http://www.herbi-systems.com/Weed_Facts/weed_facts.html
    • http://www.freepatentsonline.com/6756341.html
    • http://bioenergy.asu.edu/photosyn/education/photointro.html
    • http://georgiafaces.caes.uga.edu/getstory.cfm?storyid=2281
    • http://www.gardeningknowhow.com/children-in-the-garden/how-does-water-affect-plant-growth.htm
    • http://www.wvu.edu/~Agexten/hortcult/fruits/blkwalnt.htm
    • http://www.freepatentsonline.com/6756341.html
  • The End Thank You For Your Attention