Proteus spp.
Prepared by
Hoven Jalal
Elaf Abdulhakem
Objectves:
Morphology of proteus spp.
Microscopical morphology
Colony morphology
Diagnostic methods of proteus spp.
Gram s...
General charachteristic:
Gram negative rods, facultative anaerobics.
Motile they have peritrichous flagella
Non capsulated...
The main species of medical importance are:
P. mirabilis
P. vulgaris

Proteus spp. are opportunist pathogens and may
cause...
Morphology of proteus spp
Microscopical morphology
Gram negative bacilli, motile has flagella
Colony morphology:
Large ,circular,gray ,smooth colonies
Diagnosis method of proteus spp.
Specimens:
Urine, pus and ear
Gram stain:
Rod shaped gram negative
Culture:
Blood agar: Swarming effect over blood agar
plate as a consequence of the organisms active
motility.
Macconkey agar: Cultures give out an odour described
as fishy, Non- lactose fermenting colonies .
Oxidase test:
The oxidase test is used to determine if a
bacterium produces certain cytochrome c
oxidases. The reagent tur...
IMViC test
1-indole test:
is used to determine the ability of bacteria to
convert tryptophan into indole.
P. mirabilis can...
Methyl red test:
. The methyl red test is used to identify bacteria
to produce pyruvic acid from glucose
metabolism.
Prote...
Voges–Proskauer:
is a test used to detect acetoin in •
a bacterial broth culture. A red-brown color
indicates a positive r...
Citrate test:
Ability of an organism to use citrate as the sole
source of carbon and energy.

p.Vulgaris: Negative
p.mirab...
TSI test
Triple Sugar Iron test
This test is used to determine the ability of
bacteria to ferment sugars and to
produce hy...
Motility test
used to determine whether an organism is
equipped with flagella and thus capable of
swimming away from a sta...
Swarming phenomenon
swarming is described as the formation of
concentric zones of bacterial growth, able to
cover the whol...
API
By inoculating microorganism to a strip that has 20
tests on it. during inoculation metabolic cause
color change, to c...
Vitek system
It is a new automatic system for identification
and susceptibility testing for most of clinically
important b...
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Proteus spp (2)

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Proteus spp (2)

  1. 1. Proteus spp. Prepared by Hoven Jalal Elaf Abdulhakem
  2. 2. Objectves: Morphology of proteus spp. Microscopical morphology Colony morphology Diagnostic methods of proteus spp. Gram stain Culture &isolation on blood agar,macconkey agar Oxidase test Imvic test TSI test Motility test Swarming phenomenon Api &vitek system
  3. 3. General charachteristic: Gram negative rods, facultative anaerobics. Motile they have peritrichous flagella Non capsulated Non spore forming Proteus sp. are most commonly found in the human intestinal tract as part of normal human intestinal flora. Non-lactose fermenting
  4. 4. The main species of medical importance are: P. mirabilis P. vulgaris Proteus spp. are opportunist pathogens and may cause many types of infection.
  5. 5. Morphology of proteus spp Microscopical morphology Gram negative bacilli, motile has flagella
  6. 6. Colony morphology: Large ,circular,gray ,smooth colonies
  7. 7. Diagnosis method of proteus spp. Specimens: Urine, pus and ear Gram stain: Rod shaped gram negative
  8. 8. Culture: Blood agar: Swarming effect over blood agar plate as a consequence of the organisms active motility.
  9. 9. Macconkey agar: Cultures give out an odour described as fishy, Non- lactose fermenting colonies .
  10. 10. Oxidase test: The oxidase test is used to determine if a bacterium produces certain cytochrome c oxidases. The reagent turns dark blue when oxidized (oxidase positive). The reagent is colorless when reduced (oxidase-negative) Proteus spp. Oxidase negative
  11. 11. IMViC test 1-indole test: is used to determine the ability of bacteria to convert tryptophan into indole. P. mirabilis can be differentiated from p.vulgaris by indole test. P. mirabilis → negative P. vulgaris → positive
  12. 12. Methyl red test: . The methyl red test is used to identify bacteria to produce pyruvic acid from glucose metabolism. Proteus vulgaris: Methyl red: posative Proteus mirabils: Methyl red: posative
  13. 13. Voges–Proskauer: is a test used to detect acetoin in • a bacterial broth culture. A red-brown color indicates a positive result, while a yellowbrown color indicates a negative result. p.Vulgaris : Negative p.mirabilis:negative
  14. 14. Citrate test: Ability of an organism to use citrate as the sole source of carbon and energy. p.Vulgaris: Negative p.mirabilis: posative
  15. 15. TSI test Triple Sugar Iron test This test is used to determine the ability of bacteria to ferment sugars and to produce hydrogen sulfide (H2S) or other gases Proteus spp. (red/red with H2S production) (black)
  16. 16. Motility test used to determine whether an organism is equipped with flagella and thus capable of swimming away from a stab mark. Left tube • shows positive motility test for Proteus spp. Right tube • negative for S.aureus
  17. 17. Swarming phenomenon swarming is described as the formation of concentric zones of bacterial growth, able to cover the whole surface of solid culture medium. P.mirabilis & P.vulgaris are known for their swarming ability over(sheep blood agar)
  18. 18. API By inoculating microorganism to a strip that has 20 tests on it. during inoculation metabolic cause color change, to complete the identification of the strains either identified to the genus only or that have multiple genera consist of profile number
  19. 19. Vitek system It is a new automatic system for identification and susceptibility testing for most of clinically important bacteria. It compromises: -A filtter/sealer:enable -inoculation of the card within few minute. -An inoculator /leader - A computer and printer
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