Molecular Biology Seminary<br />Evert Jiménez <br />Medicine<br />24/08/2010<br />http://formacionbiblioteca.udea.edu.co/m...
Expression of bone morphogenetic protein-2 and<br />its receptors in epithelial ovarian cancer and their<br />influence on...
INTRODUCTION<br />This study seek determinate the expression of bone morphogenetic protein-2 (BMP-2) and its receptors BMP...
CANCER<br />Cancer is a mutation or abnormal activation of genes that control cell growth and cell mitosis which end in ex...
OVARIO<br />Is the Gonad femenine. is responsible for produce female sex hormones and ovulation. There are two with almond...
BMP-2<br />BMP is a member of the transforming<br />growth factor-β superfamily. Initially, it was thought<br />to induce ...
GENERAL OBJETIVE<br />The propose of this study is determinate the<br />expression of BMP-2 and its receptors in epithelia...
MATERIALES Y METODOS<br />RT-PCR<br />Western Blot<br />INMUNOHISTOQUIMICA<br />
MATERIALES Y METODOS<br />RT-PCR<br />Western Blot<br />RNA total extraido de 100mg especimenes<br />1.5% agarosa gel elec...
RESULTADO<br />Los niveles de expresión del ARNm de BMP-2, BMPRIB, y BMPRII en los tejidos de cáncer de ovario fue signifi...
 <br />Figure 1 The mRNA expression of BMP-2 and its receptors detected by RT-PCR 1: Ovarian cancer tissue; 2: Benign ovar...
1<br />3<br />2<br />B-actin<br />43KD<br />BMP-2<br />20KD<br />BMRIA<br />66KD<br />BMRIB<br />50KD<br />BMRII<br />75KD...
DISCUSsION<br />http://churchwhisperer.com/2009/09/01/after-much-discussion/<br />
CONCLUSION<br />
THANK`S<br />http://www.valledellili.org/sitio/index.php?option=com_content&view=article&id=446%3Aen-el-aparato-reproducto...
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Expression of bmp2 in epithelial ovarian cancer.

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Expression of bmp2 in epithelial ovarian cancer.

  1. 1. Molecular Biology Seminary<br />Evert Jiménez <br />Medicine<br />24/08/2010<br />http://formacionbiblioteca.udea.edu.co/moodle/course/view.php?id=143&topic=1<br />
  2. 2. Expression of bone morphogenetic protein-2 and<br />its receptors in epithelial ovarian cancer and their<br />influence on the prognosis of ovarian cancer<br />Patients.<br />Ma et al. Journal of Experimental & Clinical Cancer Research 2010, 29:85<br />http://www.jeccr.com/content/29/1/85<br />http://formacionbiblioteca.udea.edu.co/moodle/course/view.php?id=143&topic=1<br />
  3. 3. INTRODUCTION<br />This study seek determinate the expression of bone morphogenetic protein-2 (BMP-2) and its receptors BMPRIA,<br />BMPRIB, and BMPRII in epithelial ovarian cancer (EOC) and to analyze their influence on the prognosis of ovarian cancer<br />patients.<br />
  4. 4. CANCER<br />Cancer is a mutation or abnormal activation of genes that control cell growth and cell mitosis which end in excess of malignant cells with growth and division beyond the normal limits.<br />http://www.medicosdeelsalvador.com/Detailed/Im_genes_M_dicas/Coloproctolog_a/C_ncer_de_Ovario_946.html<br />
  5. 5. OVARIO<br />Is the Gonad femenine. is responsible for produce female sex hormones and ovulation. There are two with almond form. They connect with the uterus through the fallopian tubes and suspended by the broad ligament of ovary .<br />http://www.ferato.com/wiki/index.php/Ovario<br />
  6. 6. BMP-2<br />BMP is a member of the transforming<br />growth factor-β superfamily. Initially, it was thought<br />to induce bone formation and condrogenesisin vivo,<br />and current evidence suggests that it also participates in<br />some biological processes of cells, such as proliferation,<br />Differentiation and apoptosis.<br />http://www.treslacteospordia.org.uy/alternativas_chicos.php<br />
  7. 7. GENERAL OBJETIVE<br />The propose of this study is determinate the<br />expression of BMP-2 and its receptors in epithelial ovarian<br />cancer, benignovariantumors, and normal ovarian<br />tissue and to analyze their influence on the five-year survival<br />rate and average survival time of ovarian cancer<br />patients.<br />
  8. 8. MATERIALES Y METODOS<br />RT-PCR<br />Western Blot<br />INMUNOHISTOQUIMICA<br />
  9. 9. MATERIALES Y METODOS<br />RT-PCR<br />Western Blot<br />RNA total extraido de 100mg especimenes<br />1.5% agarosa gel electroforesis con<br />Bromuro de etidio.<br />la proteína fue extraída de 100 mgespecímenes<br />INMUNOHISTOQUIMICA<br />fueron analizadas con el método de Bradford<br />Poliacrilamida gel electroforesis<br />Parafina<br />Filtro de nitrocelulosa<br />Anticuerpos<br />
  10. 10. RESULTADO<br />Los niveles de expresión del ARNm de BMP-2, BMPRIB, y BMPRII en los tejidos de cáncer de ovario fue significativamente menor que los de los tumores benignos ováricos o normal tejido. No hubo diferencias significativas en BMPRIA ARNmniveles de expresión que se observaron entre los tres tipos de Tejidos.<br />RT-PCR<br />El contenido relativo de las proteínas BMP-2, BMPRIB, yBMPRII en el tejido de cáncer de ovario fue significativamente menorque los de los tumores benignos ováricos o normaltejido. No hubo diferencias significativas en proteínas BMPRIAnivel de expresión se observaron entre los tres tipos deTejidos.<br />Western Blot<br />INMUNOHISTOQUIMICA<br />
  11. 11.  <br />Figure 1 The mRNA expression of BMP-2 and its receptors detected by RT-PCR 1: Ovarian cancer tissue; 2: Benign ovarian tumor tissue; 3: Normal ovarian tissue; M: Marker.<br />
  12. 12. 1<br />3<br />2<br />B-actin<br />43KD<br />BMP-2<br />20KD<br />BMRIA<br />66KD<br />BMRIB<br />50KD<br />BMRII<br />75KD<br />2 The protein expression of BMP-2 and its receptors detected by western blot 1: Ovarian cancer tissue; 2: Benign ovarian tumor tissue; 3: Normal ovarian tissue.<br />
  13. 13. DISCUSsION<br />http://churchwhisperer.com/2009/09/01/after-much-discussion/<br />
  14. 14.
  15. 15. CONCLUSION<br />
  16. 16.
  17. 17. THANK`S<br />http://www.valledellili.org/sitio/index.php?option=com_content&view=article&id=446%3Aen-el-aparato-reproductor-tambien-hay-riesgo&catid=38%3Anuestras-noticias&Itemid=112&lang=es<br />

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