Biologia plegable

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Biologia plegable

  1. 1. SQUISHY SCIENCE:EXTRACT DNA FROM SMASHED STRAWBERRIES ESTEFANIA JARAMILLO SALAZAR 3rd SEMESTER MEDICINE STUDENT U.P. B FEBRUAR 11/ Y 2013
  2. 2. FOLDING MEDICAL UTILITY. SQUISHY SCIENCE: EXTRACT DNA FROMWhether you’re a human, rat, tomato or SMASHED STRAW BERRIES bacterium, each of your cells will have DNA inside of it (with some rare exceptions, such as mature red bloodcells in humans). Each cell has an entire MOLECULAR BIOLOGYcopy of the same set of instructions, andthis set is called the genome. Scientistsstudy DNA for many reasons: They can ESTEFANIA JARAMILLO figure out how the instructions stored in SALAZARDNA help your body to function properly. They can use DNA to make new TEACHERmedicines or genetically modify crops to LINA MARÍA MARTINEZ be resistant to insects. They can solve SANCHEZ who is a suspect of a crime, and can even use ancient DNA to reconstruct evolutionary histories! MEDICINE STUDENT To get the DNA from a cell, scientists BIBLIOGRAPH 3rd SEMESTER typically rely on one of many DNA Y extraction kits available from -  Scientific American, a Division of Nature America, Inc.” Squishy Science: Extract DNA biotechnology companies. During a from Smashed Strawberries.” Scientific American, DNA extraction, a detergent will cause January 31, 2013. visited on the internet portal: the cell to pop open, or lyse, so that the http://www.scientificamerican.com/article.cfm?id=squishy-science-extract-dna-from-smashed-strawberries DNA is released into solution. Then -  Agency for Science, Technology and Researchalcohol added to the solution causes the (A*STAR), Singapore. "Nucleus structure crucial DNA to precipitate out. In this activity, to understanding diseases discovered.“strawberries will be used because each S c ie nc e Da ily , 7 Feb. 2013. Web. visited on the strawberry cell has eight copies of the internet portal: http://www.sciencedaily.com/releases/2013/02/13 FEBRUARY 11/ 2013 genome, giving them a lot of DNA per 0208110255.htm cell. (Most organisms only have one genome copy per cell.)  
  3. 3. FOLDING SQUISHY SCIENCE: NUCLEUS STRUCTURE EXTRACT DNA FROM CRUCIAL TO INTRODUCTION SMASHED STRAW BERRIES UNDERSTANDING DISEASES DISCOVERED Have you ever wondered how Materials : Rubbing alcohol, Measuring cup,scientists extract DNA from an Measuring spoons, Salt, water, Dishwashing liquid, Scientists from Singapore and Germany have identified that the proteins lamin A (Lmna) andorganism? All living organisms Glass or small bowl, Cheesecloth, Funnel, Tall lamin B receptor (Lbr) are essential for holding drinking glass, Three strawberries, Resealable have DNA, which is short for plastic sandwich bag, Small glass, Bamboo silent genes in their correct position at the edge of the nucleus, in the form of heterochromatin. A deoxyribonucleic acid; it is skewer, available at most grocery stores. deviation from their normal position will cause the Procedure : Prepare the extraction liquid. - Put the basically the blueprint for strawberries into a resealable plastic bag - smash genes to malfunction, leading to heart failure, vascular disease and muscle wasting.everything that happens inside the strawberries. - Add three tablespoons of the For hundreds of years before this discovery, extraction liquid to the strawberries. - Squeeze the an organism’s cells. Overall, strawberry mixture - Pour the strawberry mixture scientists were puzzled by why heterochromatin clustered at the edge of the nucleus and how it DNA tells an organism how to from the bag into the funnel. Let it drip through the was relevant to normal cell function. This recent cheesecloth and into the tall glass until there is verydevelop and function, and is so little liquid left in the funnel - Pour the filtered discovery will enable scientists to gain a better understanding of the diseases of the heart and important that this complex strawberry liquid from the tall glass into the small muscles, and find cures for them in the future. glass. - Measure out one half cup of cold rubbing compound is found in virtually alcohol. - Tilt the jar and very slowly pour the The findings by Audrey Wang and Colin Stewart of A*STARs Institute of Medical Biology and Irina every one of its cells. In this alcohol down its side. Pour until the alcohol has Solovei, Boris Joffe and Heinrich Leonhardt of the formed approximately a one-inch-deep layer on top activity you’ll make your own of the strawberry liquid. Do not let the strawberry Ludwig Maximillian University in Munich, Germany, were recently published in the DNA extraction kit from liquid and alcohol mix. prestigious journalCell. The strawberry DNA will appear as gooeyhousehold chemicals and use it STUDENT OBSERVATION. clear/white stringy stuff.   STUDENT to separate DNA from OBSERVATION. This news is of vital importance, I think that this is a very interesting story, curious strawberries.  and funny, which is available to everyone. is a because it allow us to understand simple and effective method that provides a start to diseases and their relation with age, the DNA testing allowing a better understanding besides that it gives explanation of about the functioning of the same, opening the mind to its utility and who knows probably reaching various syndromes making possible to some discoveries that allow us to develop new to give better treatment and theories in order to apply them on life. prevention of disease.
  4. 4. INTRODUCTION INTRODUCTIONDNA is important Have you ever wondered how to all the things scientists extract DNA from an that happens in organism? All living organisms the bodys cells, have DNA, which is short for deoxyribonucleic acid; it isthis tells me how basically the blueprint forthey are develop everything that happens inside and how they an organism’s cells. Overall, DNA tells an organism how to work. develop and function, and is so important that this complex compound is found in virtually This discovery is every one of its cells. In this activity you’ll make your own an experimental DNA extraction kit from activity that household chemicals and use it works closely to separate DNA from strawberries. with DNA. It helpsits understanding, leaving the knowledge for everyone.
  5. 5. SQUISHY SCIENCE:EXTRACT DNA FROM SMASHED STRAW SCIENCE: BERRIES SQUISHY EXTRACT DNA FROM SMASHED STRAWBERRIES Materials : Rubbing alcohol, Measuring cup, Measuring spoons, Salt, water, Dishwashing liquid, Glass or small bowl, Cheesecloth, Funnel, Tall drinking glass, Three strawberries, Resealable plastic sandwich bag, Small glass, Bamboo skewer, available at most grocery stores. Procedure : Prepare the extraction liquid. - Put the strawberries into a resealable plastic bag - smash the strawberries. - Add three tablespoons of the extraction liquid to the strawberries. - Squeeze the strawberry mixture - Pour the strawberry mixture from the bag into the funnel. Let it drip through the cheesecloth and into the tall glass until there is very little liquid left in the funnel - Pour the filtered strawberry liquid from the tall glass into the small glass. - Measure out one half cup of cold rubbing alcohol. - Tilt the jar and very slowly pour the alcohol down its side. Pour until the alcohol has formed approximately a one-inch-deep layer on top of the strawberry liquid. Do not let the strawberry liquid and alcohol mix. The strawberry DNA will appear as gooey STUDENT OBSERVATION. clear/white stringy stuff.   I think that this is a very interesting story, curious and funny, which is available to everyone. is a simple and effective method that provides a start to the DNA testing allowing a better understanding about the functioning of the same, opening the mind to its utility and who knows probably reaching to some discoveries that allow us to develop new theories in order to apply them on life.
  6. 6. SQUISHY SCIENCE: EXTRACT DNA FROM SMASHED STRAW BERRIESPreparation of the extractionliquid- Chill the rubbing alcohol in thefreezer. (You’ll need it later.)- Mix one half teaspoon of salt, onethird cup of water and one tablespoonof dishwashing liquid in a glass or smallbowl. Set the mixture aside. This isyour extraction liquid. Why d o y o u thinkthe re is d e te rg e nt in the e x tra c tio nliq uid ?- Completely line the funnel withcheesecloth. Insert the funnel tube intothe tall drinking glass (not the glasswith the extraction liquid in it).- Remove and discard the green topsfrom the strawberries.
  7. 7. SQUISHY SCIENCE: EXTRACT DNA FROM SMASHED STRAW BERRIESExtra:  the experiment can be make it inseveral ways:- Grab some oatmeal or kiwis from thekitchen and try it again! Whic h fo o d s g ivey o u the m o s t DN ? A- Try to tweak different variables in thisactivity to see how you could changeyour strawberry DNA yield. For example,you could try starting with differentamounts of strawberries, using differentdetergents or different DNA sources(such as oatmeal or kiwis). Whic hc o nd itio ns g ive y o u the be s t DN y ie ld ? A
  8. 8. SQUISHY SCIENCE: EXTRACT DNA FROM SMASHED STRAW BERRIESMaterialsRubbing alcohol, Measuring cup,Measuring, spoons, Salt, water,Dishwashing liquid (for hand-washing dishes), Glass or smallbowl, Cheesecloth, Funnel, Talldrinking glass, Three strawberries,Resealable plastic sandwich bag,Small glass jar, (such as a spiceor baby food jar), Bamboo skewer,available at most grocery stores.(If you use a baby food or shortspice jar, you could substitute atoothpick for the skewer.)
  9. 9. OBSERVATIO NI think that this is a very interesting story, curious and funny, which is available to everyone. is a simple and effective method that provides a start to the DNA testing allowing a better understanding about the functioning of the same, opening the mind to its utility and who knows probably reaching to somediscoveries that allow us to develop new theories in order to apply them on life.
  10. 10. NUCLEUS STRUCTURE CRUCIAL TOUNDERSTANDING DISEASES DISCOVERED NUCLEUS STRUCTURE CRUCIAL TO UNDERSTANDING DISEASES DISCOVERED Scientists from Singapore and Germany have identified that the proteins lamin A (Lmna) and lamin B receptor (Lbr) are essential for holding silent genes in their correct position at the edge of the nucleus, in the form of heterochromatin. A deviation from their normal position will cause the genes to malfunction, leading to heart failure, vascular disease and muscle wasting. For hundreds of years before this discovery, scientists were puzzled by why heterochromatin clustered at the edge of the nucleus and how it was relevant to normal cell function. This recent discovery will enable scientists to gain a better understanding of the diseases of the heart and muscles, and find cures for them in the future. The findings by Audrey Wang and Colin Stewart of A*STARs Institute of Medical Biology and Irina Solovei, Boris Joffe and Heinrich Leonhardt of the Ludwig Maximillian University in Munich, Germany, were recently published in the prestigious journalCell. STUDENT OBSERVATION. This news is of vital importance, because it allow us to understand diseases and their relation with age, besides that it gives explanation of various syndromes making possible to give better treatment and prevention of disease.
  11. 11. NUCLEUS STRUCTURE CRUCIAL TOUNDERSTANDING DISEASES DISCOVERED The nucleus has the information as of chromatinnecessary for the cell to grow, develop and reproduce. scientists showed that AYB like sheet proteins areimportant for the organizationof chromatin in the nucleus. ifthere is a lack of these will be a collapse in the nuclearcenter causing a disruption of gene expression and development of affected skeletal muscle, resulting in muscle failure.
  12. 12. NUCLEUS STRUCTURE CRUCIAL TOUNDERSTANDING DISEASES DISCOVERED the findings on changes in the position of chromatin cancontribute to the exploration of heart failure.
  13. 13. NUCLEUS STRUCTURE CRUCIAL TO UNDERSTANDING DISEASES DISCOVERED This news takes researchersa step closer to understanding the nucleus and the development of new treatments for common diseases. It could be particularly relevant for Mexico, since, like other developed countries, are facing the population aging,heart failure, vascular disease and muscle atrophy that increase with age.
  14. 14. NUCLEUS STRUCTURE CRUCIAL TO UNDERSTANDING DISEASES DISCOVEREDTwo major advances have been made in the last years, theexperimentation with thecloning and the discovery of the Human Genome Mapping considered the greatest history feat
  15. 15. OBSERVATIO N This news is of vital importance,because it allow us to understand diseases and their relation with age, besides that it givesexplanation of various syndromes making possible to give better treatment and prevention of disease.
  16. 16. MEDICAL UTILITY MEDICAL UTILITY. Whether you’re a human, rat, tomato or bacterium, each of your cells will have DNA inside of it (with some rare exceptions, such as mature red blood cells in humans). Each cell has an entire copy of the same set of instructions, and this set is called the genome. Scientists study DNA for many reasons: They can figure out how the instructions stored in DNA help your body to function properly. They can use DNA to make new medicines or genetically modify crops to be resistant to insects. They can solve who is a suspect of a crime, and can even use ancient DNA to reconstruct evolutionary histories! To get the DNA from a cell, scientists typically rely on one of many DNA extraction kits available from biotechnology companies. During a DNA extraction, a detergent will cause the cell to pop open, or lyse, so that the DNA is released into solution. Then alcohol added to the solution causes the DNA to precipitate out. In this activity, strawberries will be used because each strawberry cell has eight copies of the genome, giving them a lot of DNA per cell. (Most organisms only have one genome copy per cell.)
  17. 17. MEDICAL UTILITYAllow us to havea more empirical knowledgeabout DNA and its importance
  18. 18. MEDICAL UTILITY Allow us to have a more consciousunderstanding of the importance of thecorrect position of thegenes in the nucleusand which may cause abnormalities in the same, like a malfunction of thehearts blood vessels and muscles
  19. 19. BIBLIOGRAPH Y-  Scientific American, a Division of Nature America, Inc.”Squishy Science: Extract DNA from Smashed Strawberries.”Scientific American, January 31, 2013. visited on the internetportal:http://www.scientificamerican.com/article.cfm?id=squishy-science-extract-dna-from-smashed-strawberries-  Agency for Science, Technology and Research (A*STAR),Singapore. "Nucleus structure crucial to understandingdiseases discovered.“ Sc ie nc e Da ily , 7 Feb. 2013.Web. visited on the internet portal:http://www.sciencedaily.com/releases/2013/02/130208110255.htm 
  20. 20. THANKYOU FOR YOUR ATTENTION ¡

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