Pyruvate kinase catalyzes the phosphorylation of ADP to ATP with the formation of pyruvate . Pyruvate then reduces any NADH present to NAD with formation of lactate. Loss of florescence of NADH under ultra-violet light is observed as evidence of the presence of pyruvate kinase.
phosphoenol pyruvate pyruvate lactate PK ADP ATP *NADH NAD
an acquired clonal disorder of stem cells, and results from a somatic mutation in phosphatidylinositol glycan A (PIGA) gene that is essential for the synthesis of the glycosylphosphatidylinositol (GPI) anchor protein of the membrane, and makes RBCs very sensitive to complement-mediated cell lysis due to the deficiency of proteins that regulate complement activity
GPI-linked complement reg. Proteins CD55 (DAF) CD59 CD8 binding protein