Antesense rna

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Antesense rna

  1. 1. ANTISENSE RNA<br />Desh Bandhu<br /> 10/pbt/006 <br />
  2. 2. INTRODUCTION<br />Antisense RNA is a single-stranded RNA that is complementary to a messenger RNA (mRNA) strand transcribed within a cell.<br />Antisense RNA may be introduced into a cell to inhibit translation of a complementary mRNA by base pairing to it and physically obstructing the translation machinery.<br />An example of naturally occurring mRNA antisense mechanism is the hok/sok system of the E.coli R1 plasmid.<br />
  3. 3. INVITRO MECHANISM<br />
  4. 4. Hok/sok system(Natural mechanism)<br />The hok/sok system involves three genes:<br />hok, host killing - a long lived (half-life 20 minutes) toxin.<br />sok, suppression of killing - a short lived (half-life 30 seconds) RNA antitoxin<br />mok, modulation of killing - required for hok translation<br />
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  6. 6. ROLE OF ANTESENSE RNA<br />To inactivate the polygalacturonase gene.<br />To inactivate ethylene synthesis.<br />Reduction of starch content in vegetables.<br />Modification of flower colour in various decorative plant.<br />Mammalian genomes encode numerous natural antisense transcripts, but the function of these transcripts is not well understood.<br />
  7. 7. FUTURE PROSPECTS<br />In Gene therapy.<br />To develop more effective therapies for infection with certain virus such as HIV and hepapetitis B virus.<br />To reduced the elevated blood pressure.<br />

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