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The termiticidal potential of c. odorata
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The termiticidal potential of c. odorata

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.The termiticidal potential of c. odorata.

.The termiticidal potential of c. odorata.

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The termiticidal potential of c. odorata The termiticidal potential of c. odorata Presentation Transcript

  • Researchers:Research Adviser: TESSIE Q. PERALTA, Ph. D. Don Mariano Marcos Memorial State University South La Union Campus College of Education LABORATORY HIGH SCHOOL Agoo, La Union 2009 CHEMREZ Green Chemistry Award [SIBOL Category]
  • THE PROBLEM &ITS BACKGROUND 4
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  • Chromolaena odorata (HAGONOY)> a weed belonging to the Asteraceae family> occurs in agricultural areas, naturalforests, planted forests, range /grasslands,riparian zones, ruderal /disturbed, scrub/shrublands> considered invasive weed of field crops inits introduced range, and has been reportedto be the most problematic invasive species> reduces the carrying capacity of the fieldto grow more beneficial weeds (allelophatic)> causes diarrhea when ingested or evenfetal deaths among cows> contains carcinogenic pyrrolizidinealkaloids (hepatotoxic-potentially damagingto the liver). The bright green regions indicate where Chromolaena has invaded forest gaps and 6 replaced the forest.
  • TERMITES> group of social insects usually classifiedat the taxonomic rank of order Isoptera> prefer to feed on dead plant material,wood, leaf litter, or soil> live in colonies; prime example ofdecentralized, organized systems using swarmintelligence and use this cooperation to exploitfood sources and environments that could notbe available to any single insect acting alone> typical termite colony contains nymphs(semi-mature young), workers, soldiers, andreproductive individuals of both sexes,sometimes containing several egg-layingqueens [insect castes]
  • 4 Major Termite Species in the Philippines Nasutitermes luzonicus (Luzon Point Headed termite) Coptotermes vastator (Philippine Milk termite) Microcerotermes losbanosensis (Los Banos termite) Macrotermes gilvus (Mound Building termite) 8
  • Additional Expenses to Repair DamagedStructural Damage Properties TERMITES Destructive Use of Expensive Chemical Termiticide Lack of Knowledge of Plant Alternative as Termiticide 9
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  • Risk to Public Health and Environment Expensive Pesticide Toxicity to Natural EnemiesResistance and Other Non-target Organisms DEPENDENCE ON INSECTICIDES Highly Easy to Use Effective Readily Lack of Knowledge on its Impacts to Health Available and Environment 11
  • Objectives of the Study1. To test termiticidal potential of C. odorata in terms of the length of time the termites are killed after the application of the five treatments.2. To determine the significant differences in the length of time the termites are killed when exposed to the five treatments.3. To analyze the significant differences in the length of time the termites are killed between pairs of treatments. 12
  • Null HypothesesThe following statements were tested experimentally at .05 level of significance:1. There are no significant differences in the length of time the termites are killed after the application of the five treatments.2. There are no significant differences in the length of time the termites are killed between pairs of treatments. 13
  • Conceptual ParadigmINDEPENDENT VARIABLE DEPENDENT VARIABLE 14
  • Significance of the StudyResults of the study may be significant to the following: a. People and Industry b. Other Researchers c. The Researchers 15
  • RESEARCH METHODS
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  • a. plant material b. Test organisms c. Laboratory apparatuses andd. chemicals, etc equipments 18
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  • Rotary Evaporator 20http://chem-ilp.net/labTechniques/RotaryEvaporatorApparatus.htm
  • Rotavation 21
  • Rotary Evaporation C. odorata MeOH leaf extractMethanol 22
  • Hagonoy Methanol Leaf Extract Solutions (sample computation) 30% MeOH Leaf Extract Solution 60% MeOH Leaf Extract Solution (100 mL) (100 mL) 100 mL 30% MeOH Leaf 100 mL 60% MeOH Leaf Extract Solution Extract Solution + = + =30 mL pure hagonoy 70 mL H20 60 mL pure hagonoy 40 mL H20 MeOH leaf extract MeOH leaf extract 23
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  • The test organisms as affected by the various treatmentsT1 - positive control T2 – leaf decoction T3 – expressed leaf juice T4 – 30% hagonoy methanol T5 – 60% hagonoy methanol 25 leaf extract solution leaf extract solution
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  • DATA GATHERED:Length of time in minutes for the termites to die due to various treatments
  • One-Way ANOVA Summary Table Source of Degrees of Sum of Mean F-ratio Variation Freedom Squares Square Between 4 1.39 0.35 38.98*Treatments Within 10 0.09 0.009Treatments TOTAL 14 1.48Legend: *significant at ∞ =.05 CV = 5.83 % 29 Click here to see sample computation
  • Pairwise Comparison of the Mean Time using Scheffés Test Between F’ F(.05)(k-1) ∞ = .05 Treatments T1 vs. T2 1 896.3 13.44 significant T1 vs. T3 9 074.1 13.44 significant T1 vs. T4 7.41 13.44 not significant T1 vs. T5 474 13.44 significant T2 vs. T3 2 674 13.44 significant T2 vs. T4 1 666.7 13.44 significant T2 vs. T5 4 266.7 13.44 significant T3 vs. T4 8 563 13.44 significant T3 vs. T5 13 696.3 13.44 significant T4 vs. T5 600 13.44 significant 30
  • Null Hypotheses1. There are no significant differences in the length of time the termites are killed after the application of the five treatments.2. There are no significant differences in the length of time the termites are killed between pairs of treatments.Decision: Reject Ho 31
  • Conclusions:• 60% C. odorata leaf extract solution is effective in killing the sample termites with the shortest length of time• The positive control and 30% C. odorata leaf extract solution are not significantly different from each other 32
  • Conclusions:• There are significant differences between the pairwise comparisons of: • T1 vs. T2 • T2 vs. T5 • T1 vs. T3 • T3 vs. T4 • T1 vs. T5 • T3 vs. T5 • T2 vs. T3 • T4 vs. T5 • T2 vs. T4• T1 vs. T4 = not significant between .05 level 33
  • Recommendations:• C. odorata be the best alternative termiticide for it is health and environment friendly.• Leaf decoction and expressed leaf juice are better to use for practical reasons. It is more economical to use and easy to obtain. 34
  • Recommendations:• Further studies should be conducted > utilize the same preparations of C.odorata to investigate its effects on other type of pest (ants, bedbugs, cockroaches, mosquitoes, flies, mice, rats, etc.) as well as on plants (weeds, herbs, etc.) > oil extraction and fractionation of the various chemical constituents of C. odorata to analyze which of these substances have the potential to cause such termiticidal effect 35
  • Recommendations:• Other researchers may employ the similar arrangement and set-up of treatments of the study using variety of plants in analyzing and determining their potential not only as termiticide but also another type of pesticide• Develop a patented termiticidal product out of C. odorata which is efficient, cheap, economical, organic, and environmental and health friendly 36