First Biology on SANS2d
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First Biology on SANS2d



Talk given at the Neutrons in Biology Meeting, held at Santa Fe, 24-29 October 2009

Talk given at the Neutrons in Biology Meeting, held at Santa Fe, 24-29 October 2009



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First Biology on SANS2d First Biology on SANS2d Presentation Transcript

  • First Biology on SANS2D Will accelerator source time-of-flight SANS deliver for structural biology? Cameron Neylon
  • £300 investment
  • Five years…so far…
  • 4 Q  sin(  / 2 ) 
  • A range of wavelengths…
  • …to access more lengthscales
  • Wikimedia Commons Succinate_Dehydrogenase_1YQ3_and_Membrane.png Biology is complicated… Wikimedia Commons MGMT BDNA_1T38.png
  • …on a wide range… William Crochot/NIST Wikimedia Commons, used with permission
  • …of lengthscales Wikimedia Commons: Yeast_membrane_proteins.jpg
  • SANS is good for complex systems Wu et al., JBC, 2009, Manuscript M109.039537
  • So TOF SANS is great for biology?
  • But when we look for examples…
  • That’s an overstatement but…
  • Secretory IgA2 Bonner et al, J. BiolChem (2009) 284:5077 SANS data from LOQ
  • …few examples of TOF-SANS used in model building
  • Why?
  • Flux/Collection time
  • Background issues
  • The source
  • Low power…
  • …more neutrons
  • The instrument The instrument
  • Collimation L1 = 2 to 12 m, Sample at 19m from moderator, Sample to detector L2 = 2 to 12 m, Qmin~ 0.002 Å-1, λ = 1.5 to 12 Å by time of flight, Qmax~ 3 Å-1 STFC
  • STFC/Richard Heenan
  • STFC/Richard Heenan
  • Adjustable collimation without windows STFC
  • STFC/Richard Heenan
  • STFC/Richard Heenan
  • STFC/Richard Heenan
  • STFC/Richard Heenan
  • Two detectors for wide Q range STFC
  • STFC/Richard Heenan
  • The data
  • Caveats Thick-walled moderator at low pressure Data from one detector only Instrument not fully aligned; collimation issues Issues with data reduction software slowing commissioning down
  • Lysozyme/D2O 8 mg.mL-1 3 hours L2 = 6m 6m collimation
  • Lysozyme/H2O
  • GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 Å-1
  • GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 Å-1 …one detector, one position
  • Problems… Issues with background subtraction Systematic errors in reduction process Instrument scattering artefacts Data reduction issues slowing optimization
  • Prospects… Enormous simultaneous Q range Two-fold flux increase from source Two-fold increase from alignment and collimation Three-fold from additional detector
  • Conclusions… Lots of work to identify instrument configurations A versatile instrument for hierarchical systems Competitive collection times wrtbest instruments Real potential for structural biology
  • NIMROD NearandInterMediateRange OrderDiffractometer STFC
  • Data from a mesostructured glass STFC Currently optimized Q-range of 0.02Å-1 to 50Å-1
  • 5Å 10Å 40Å 70Å STFC/Daniel Bowron 1Å-1 0.6Å-1 0.15Å-1 0.09Å-1
  • Prospects… World class SANS instrument for large samples High resolution data (but not verylow Q) Requires new data analysis techniques Combined data sets with differential labeling?
  • Reflectometers…
  • Reflectometers…
  • LMX ~2015
  • TS-2 and the biosciences Optimizedinstrumentation on a unique source Powerful tools for hierarchical systems Combining the best characteristics of instruments A view towards a future of long pulse sources?
  • Acknowledgements SANS2d: Richard Heenan, David Turner, James Treadgold, Sarah Rogers, Ann Terry & Steve King Sample preparation: Luke Clifton Data reduction: The MANTID team UK Large Facilities Capital Fund STFC