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First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
First Biology on SANS2d
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First Biology on SANS2d

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Talk given at the Neutrons in Biology Meeting, held at Santa Fe, 24-29 October 2009

Talk given at the Neutrons in Biology Meeting, held at Santa Fe, 24-29 October 2009

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  • 1. First Biology on SANS2D Will accelerator source time-of-flight SANS deliver for structural biology? Cameron Neylon
  • 2. £300 investment http://www.flickr.com/photos/smigol/2467443756
  • 3. Five years…so far… http://www.flickr.com/photos/joelanman/366972076
  • 4. 4 Q  sin(  / 2 ) 
  • 5. A range of wavelengths… http://www.flickr.com/photos/7519597@N05/2466444043
  • 6. …to access more lengthscales http://www.flickr.com/photos/smithco/76749100
  • 7. Wikimedia Commons Succinate_Dehydrogenase_1YQ3_and_Membrane.png Biology is complicated… Wikimedia Commons MGMT BDNA_1T38.png
  • 8. …on a wide range… William Crochot/NIST Wikimedia Commons, used with permission
  • 9. …of lengthscales Wikimedia Commons: Yeast_membrane_proteins.jpg
  • 10. SANS is good for complex systems Wu et al., JBC, 2009, Manuscript M109.039537
  • 11. So TOF SANS is great for biology? http://www.flickr.com/photos/thetruthabout/2842830409
  • 12. But when we look for examples… http://www.flickr.com/photos/suzanneandsimon/1561911861
  • 13. That’s an overstatement but…
  • 14. Secretory IgA2 Bonner et al, J. BiolChem (2009) 284:5077 SANS data from LOQ
  • 15. …few examples of TOF-SANS used in model building
  • 16. Why? http://www.flickr.com/photos/mattymatt/3017263513
  • 17. Flux/Collection time
  • 18. http://www.flickr.com/photos/fotoosvanrobin/3470106225
  • 19. Background issues http://www.flickr.com/photos/god-country-history/1535244721
  • 20. http://www.flickr.com/photos/akira_kev/2307577638
  • 21. The source http://www.flickr.com/photos/scobleizer/2442984975
  • 22. Low power… http://www.flickr.com/photos/wheany/3200055138
  • 23. …more neutrons
  • 24. The instrument The instrument http://www.flickr.com/photos/cellonaut/3225137280
  • 25. Collimation L1 = 2 to 12 m, Sample at 19m from moderator, Sample to detector L2 = 2 to 12 m, Qmin~ 0.002 Å-1, λ = 1.5 to 12 Å by time of flight, Qmax~ 3 Å-1 STFC
  • 26. STFC/Richard Heenan
  • 27. STFC/Richard Heenan
  • 28. Adjustable collimation without windows STFC
  • 29. STFC/Richard Heenan
  • 30. STFC/Richard Heenan
  • 31. STFC/Richard Heenan
  • 32. STFC/Richard Heenan
  • 33. Two detectors for wide Q range STFC
  • 34. STFC/Richard Heenan
  • 35. The data
  • 36. Caveats Thick-walled moderator at low pressure Data from one detector only Instrument not fully aligned; collimation issues Issues with data reduction software slowing commissioning down
  • 37. Lysozyme/D2O 8 mg.mL-1 3 hours L2 = 6m 6m collimation
  • 38. Lysozyme/H2O
  • 39. GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 Å-1
  • 40. GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 Å-1 …one detector, one position
  • 41. Problems… Issues with background subtraction Systematic errors in reduction process Instrument scattering artefacts Data reduction issues slowing optimization
  • 42. Prospects… Enormous simultaneous Q range Two-fold flux increase from source Two-fold increase from alignment and collimation Three-fold from additional detector
  • 43. Conclusions… Lots of work to identify instrument configurations A versatile instrument for hierarchical systems Competitive collection times wrtbest instruments Real potential for structural biology
  • 44. NIMROD NearandInterMediateRange OrderDiffractometer STFC
  • 45. Data from a mesostructured glass STFC Currently optimized Q-range of 0.02Å-1 to 50Å-1
  • 46. 5Å 10Å 40Å 70Å STFC/Daniel Bowron 1Å-1 0.6Å-1 0.15Å-1 0.09Å-1
  • 47. Prospects… World class SANS instrument for large samples High resolution data (but not verylow Q) Requires new data analysis techniques Combined data sets with differential labeling?
  • 48. Reflectometers…
  • 49. Reflectometers…
  • 50. LMX ~2015
  • 51. TS-2 and the biosciences Optimizedinstrumentation on a unique source Powerful tools for hierarchical systems Combining the best characteristics of instruments A view towards a future of long pulse sources?
  • 52. http://www.flickr.com/photos/thetruthabout/2842830409
  • 53. Acknowledgements SANS2d: Richard Heenan, David Turner, James Treadgold, Sarah Rogers, Ann Terry & Steve King Sample preparation: Luke Clifton Data reduction: The MANTID team UK Large Facilities Capital Fund STFC

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