First Biology on SANS2d

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Talk given at the Neutrons in Biology Meeting, held at Santa Fe, 24-29 October 2009

Talk given at the Neutrons in Biology Meeting, held at Santa Fe, 24-29 October 2009

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  • 1. First Biology on SANS2D Will accelerator source time-of-flight SANS deliver for structural biology? Cameron Neylon
  • 2. £300 investment http://www.flickr.com/photos/smigol/2467443756
  • 3. Five years…so far… http://www.flickr.com/photos/joelanman/366972076
  • 4. 4 Q  sin(  / 2 ) 
  • 5. A range of wavelengths… http://www.flickr.com/photos/7519597@N05/2466444043
  • 6. …to access more lengthscales http://www.flickr.com/photos/smithco/76749100
  • 7. Wikimedia Commons Succinate_Dehydrogenase_1YQ3_and_Membrane.png Biology is complicated… Wikimedia Commons MGMT BDNA_1T38.png
  • 8. …on a wide range… William Crochot/NIST Wikimedia Commons, used with permission
  • 9. …of lengthscales Wikimedia Commons: Yeast_membrane_proteins.jpg
  • 10. SANS is good for complex systems Wu et al., JBC, 2009, Manuscript M109.039537
  • 11. So TOF SANS is great for biology? http://www.flickr.com/photos/thetruthabout/2842830409
  • 12. But when we look for examples… http://www.flickr.com/photos/suzanneandsimon/1561911861
  • 13. That’s an overstatement but…
  • 14. Secretory IgA2 Bonner et al, J. BiolChem (2009) 284:5077 SANS data from LOQ
  • 15. …few examples of TOF-SANS used in model building
  • 16. Why? http://www.flickr.com/photos/mattymatt/3017263513
  • 17. Flux/Collection time
  • 18. http://www.flickr.com/photos/fotoosvanrobin/3470106225
  • 19. Background issues http://www.flickr.com/photos/god-country-history/1535244721
  • 20. http://www.flickr.com/photos/akira_kev/2307577638
  • 21. The source http://www.flickr.com/photos/scobleizer/2442984975
  • 22. Low power… http://www.flickr.com/photos/wheany/3200055138
  • 23. …more neutrons
  • 24. The instrument The instrument http://www.flickr.com/photos/cellonaut/3225137280
  • 25. Collimation L1 = 2 to 12 m, Sample at 19m from moderator, Sample to detector L2 = 2 to 12 m, Qmin~ 0.002 Å-1, λ = 1.5 to 12 Å by time of flight, Qmax~ 3 Å-1 STFC
  • 26. STFC/Richard Heenan
  • 27. STFC/Richard Heenan
  • 28. Adjustable collimation without windows STFC
  • 29. STFC/Richard Heenan
  • 30. STFC/Richard Heenan
  • 31. STFC/Richard Heenan
  • 32. STFC/Richard Heenan
  • 33. Two detectors for wide Q range STFC
  • 34. STFC/Richard Heenan
  • 35. The data
  • 36. Caveats Thick-walled moderator at low pressure Data from one detector only Instrument not fully aligned; collimation issues Issues with data reduction software slowing commissioning down
  • 37. Lysozyme/D2O 8 mg.mL-1 3 hours L2 = 6m 6m collimation
  • 38. Lysozyme/H2O
  • 39. GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 Å-1
  • 40. GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 Å-1 …one detector, one position
  • 41. Problems… Issues with background subtraction Systematic errors in reduction process Instrument scattering artefacts Data reduction issues slowing optimization
  • 42. Prospects… Enormous simultaneous Q range Two-fold flux increase from source Two-fold increase from alignment and collimation Three-fold from additional detector
  • 43. Conclusions… Lots of work to identify instrument configurations A versatile instrument for hierarchical systems Competitive collection times wrtbest instruments Real potential for structural biology
  • 44. NIMROD NearandInterMediateRange OrderDiffractometer STFC
  • 45. Data from a mesostructured glass STFC Currently optimized Q-range of 0.02Å-1 to 50Å-1
  • 46. 5Å 10Å 40Å 70Å STFC/Daniel Bowron 1Å-1 0.6Å-1 0.15Å-1 0.09Å-1
  • 47. Prospects… World class SANS instrument for large samples High resolution data (but not verylow Q) Requires new data analysis techniques Combined data sets with differential labeling?
  • 48. Reflectometers…
  • 49. Reflectometers…
  • 50. LMX ~2015
  • 51. TS-2 and the biosciences Optimizedinstrumentation on a unique source Powerful tools for hierarchical systems Combining the best characteristics of instruments A view towards a future of long pulse sources?
  • 52. http://www.flickr.com/photos/thetruthabout/2842830409
  • 53. Acknowledgements SANS2d: Richard Heenan, David Turner, James Treadgold, Sarah Rogers, Ann Terry & Steve King Sample preparation: Luke Clifton Data reduction: The MANTID team UK Large Facilities Capital Fund STFC