First Biology on SANS2d

First Biology on SANS2D Will accelerator source time-of-flight SANS deliver for structural biology? Cameron Neylon

£300 investment http://www.flickr.com/photos/smigol/2467443756

Five years…so far… http://www.flickr.com/photos/joelanman/366972076

4 Q  sin(  / 2 ) 

A range of wavelengths… http://www.flickr.com/photos/7519597@N05/2466444043

…to access more lengthscales http://www.flickr.com/photos/smithco/76749100

Wikimedia Commons Succinate_Dehydrogenase_1YQ3_and_Membrane.png Biology is complicated… Wikimedia Commons MGMT BDNA_1T38.png

…on a wide range… William Crochot/NIST Wikimedia Commons, used with permission

…of lengthscales Wikimedia Commons: Yeast_membrane_proteins.jpg

SANS is good for complex systems Wu et al., JBC, 2009, Manuscript M109.039537

So TOF SANS is great for biology? http://www.flickr.com/photos/thetruthabout/2842830409

But when we look for examples… http://www.flickr.com/photos/suzanneandsimon/1561911861

That’s an overstatement but…

Secretory IgA2 Bonner et al, J. BiolChem (2009) 284:5077 SANS data from LOQ

…few examples of TOF-SANS used in model building

Why? http://www.flickr.com/photos/mattymatt/3017263513

Flux/Collection time

http://www.flickr.com/photos/fotoosvanrobin/3470106225

Background issues http://www.flickr.com/photos/god-country-history/1535244721

http://www.flickr.com/photos/akira_kev/2307577638

The source http://www.flickr.com/photos/scobleizer/2442984975

Low power… http://www.flickr.com/photos/wheany/3200055138

…more neutrons

The instrument The instrument http://www.flickr.com/photos/cellonaut/3225137280

Collimation L1 = 2 to 12 m, Sample at 19m from moderator, Sample to detector L2 = 2 to 12 m, Qmin~ 0.002 Å-1, λ = 1.5 to 12 Å by time of flight, Qmax~ 3 Å-1 STFC

STFC/Richard Heenan

Adjustable collimation without windows STFC

STFC/Richard Heenan

Two detectors for wide Q range STFC

STFC/Richard Heenan

The data

Caveats Thick-walled moderator at low pressure Data from one detector only Instrument not fully aligned; collimation issues Issues with data reduction software slowing commissioning down

Lysozyme/D2O 8 mg.mL-1 3 hours L2 = 6m 6m collimation

Lysozyme/H2O

GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 Å-1

GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 Å-1 …one detector, one position

Problems… Issues with background subtraction Systematic errors in reduction process Instrument scattering artefacts Data reduction issues slowing optimization

Prospects… Enormous simultaneous Q range Two-fold flux increase from source Two-fold increase from alignment and collimation Three-fold from additional detector

Conclusions… Lots of work to identify instrument configurations A versatile instrument for hierarchical systems Competitive collection times wrtbest instruments Real potential for structural biology

NIMROD NearandInterMediateRange OrderDiffractometer STFC

Data from a mesostructured glass STFC Currently optimized Q-range of 0.02Å-1 to 50Å-1

5Å 10Å 40Å 70Å STFC/Daniel Bowron 1Å-1 0.6Å-1 0.15Å-1 0.09Å-1

Prospects… World class SANS instrument for large samples High resolution data (but not verylow Q) Requires new data analysis techniques Combined data sets with differential labeling?

Reflectometers…

LMX ~2015

TS-2 and the biosciences Optimizedinstrumentation on a unique source Powerful tools for hierarchical systems Combining the best characteristics of instruments A view towards a future of long pulse sources?

http://www.flickr.com/photos/thetruthabout/2842830409

Acknowledgements SANS2d: Richard Heenan, David Turner, James Treadgold, Sarah Rogers, Ann Terry & Steve King Sample preparation: Luke Clifton Data reduction: The MANTID team UK Large Facilities Capital Fund STFC

First Biology on SANS2d

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