0
Biotechnology A process that uses bacteria or other microorganisms to create a product for industry
Restriction Enzymes <ul><li>Viruses embed their DNA into bacteria using restriction enzymes. </li></ul><ul><li>Bacteria ne...
Blunt end cuts A  T  T  C  G  T  A  C  T  T  T  C  G  T  A T  A  A  G  C  A  T  G A  A  A  G  C A  T 
Blunt end cuts VIRUS!!
Sticky end cuts A  T  T  G  A  A  T  T  C  T  T  G  G C  T  T  A  A  C  T  T  A  A  G  A  A C  C  G A 
Action of Restriction Enzyme VIRUS !
Close-up of infected DNA What if this section of DNA is viral DNA instead of bacterial?
Bacterial defense <ul><li>Bacteria cut out the viral DNA using the same enzymes that the virus uses to embed the DNA. </li...
How industry uses restriction enzymes GENE X Insert Gene X into bacteria Use bacteria to make copies of Gene X
How to isolate “gene of interest” GENE Restriction enzyme site Restriction enzyme site
Cutting the gene out tube of restriction enzyme tube of DNA
Preparing bacteria PLASMID  Cut the bacteria with the same restriction enzyme used to cut out the gene of interest
“ Subcloning” gene of interest GENE
Cloning gene of interest Allow bacteria to grow
Obtaining gene from bacteria <ul><li>Cut bacterial DNA with restriction enzyme again. </li></ul><ul><li>Load cut DNA into ...
Pouring gel
Loading gel
Setting voltage gradient
Isolating gene Each band represents DNA pieces of different sizes.  One of these bands should have the gene of interest.
Sample gel of  Salmonella
Paternity test
Sickle cell anemia
Gels show relationship <ul><li>Gel electrophoresis, restriction enzymes, and biotechnology are helping scientists establis...
In what ways can this technology help or hurt man?
Upcoming SlideShare
Loading in...5
×

Biotechnology

685

Published on

Published in: Technology
0 Comments
0 Likes
Statistics
Notes
  • Be the first to comment

  • Be the first to like this

No Downloads
Views
Total Views
685
On Slideshare
0
From Embeds
0
Number of Embeds
0
Actions
Shares
0
Downloads
49
Comments
0
Likes
0
Embeds 0
No embeds

No notes for slide

Transcript of "Biotechnology"

  1. 1. Biotechnology A process that uses bacteria or other microorganisms to create a product for industry
  2. 2. Restriction Enzymes <ul><li>Viruses embed their DNA into bacteria using restriction enzymes. </li></ul><ul><li>Bacteria need to cut out viral DNA in self defense. </li></ul><ul><li>Bacteria use restriction enzymes as defense against viruses. </li></ul>Image of rhinovirus
  3. 3. Blunt end cuts A T T C G T A C T T T C G T A T A A G C A T G A A A G C A T 
  4. 4. Blunt end cuts VIRUS!!
  5. 5. Sticky end cuts A T T G A A T T C T T G G C T T A A C T T A A G A A C C G A 
  6. 6. Action of Restriction Enzyme VIRUS !
  7. 7. Close-up of infected DNA What if this section of DNA is viral DNA instead of bacterial?
  8. 8. Bacterial defense <ul><li>Bacteria cut out the viral DNA using the same enzymes that the virus uses to embed the DNA. </li></ul>
  9. 9. How industry uses restriction enzymes GENE X Insert Gene X into bacteria Use bacteria to make copies of Gene X
  10. 10. How to isolate “gene of interest” GENE Restriction enzyme site Restriction enzyme site
  11. 11. Cutting the gene out tube of restriction enzyme tube of DNA
  12. 12. Preparing bacteria PLASMID  Cut the bacteria with the same restriction enzyme used to cut out the gene of interest
  13. 13. “ Subcloning” gene of interest GENE
  14. 14. Cloning gene of interest Allow bacteria to grow
  15. 15. Obtaining gene from bacteria <ul><li>Cut bacterial DNA with restriction enzyme again. </li></ul><ul><li>Load cut DNA into gel for separation. </li></ul>
  16. 16. Pouring gel
  17. 17. Loading gel
  18. 18. Setting voltage gradient
  19. 19. Isolating gene Each band represents DNA pieces of different sizes. One of these bands should have the gene of interest.
  20. 20. Sample gel of Salmonella
  21. 21. Paternity test
  22. 22. Sickle cell anemia
  23. 23. Gels show relationship <ul><li>Gel electrophoresis, restriction enzymes, and biotechnology are helping scientists establish genetic relationships between different species. </li></ul>
  24. 24. In what ways can this technology help or hurt man?
  1. A particular slide catching your eye?

    Clipping is a handy way to collect important slides you want to go back to later.

×