How to Prepare an Agar Culture
Medium
Objectives:
At the end of this presentation, one must be able to:
 Prepare an Agar Petri dish using the right
procedures
...
Introduction:
Agar is a gelatinous substance perfect for
biological experiments since it holds up bacteria and
does not di...
Procedures:
 1 Begin by sterilizing the Petri dishes. Place in a

bath of boiling water and allow them to dry upside
down...
Procedures:
 2 Prepare agar powder by placing it in the

microwave with the appropriate amount of water.
The label should...
Procedures:

 3 Treat bottled agar formula carefully by loosening

the bottle cap (but not removing) before placing in
th...
Procedures:
 4 Place the Petri dishes the right way up and crack

but do not remove the lids. This is to prepare them
for...
Procedures:
 6 Pour an amount of agar liquid into each dish as

you hover the lid. About 1/8 inch is the standard
thickne...
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Agar Culture Medium

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Agar Culture Medium

  1. 1. How to Prepare an Agar Culture Medium
  2. 2. Objectives: At the end of this presentation, one must be able to:  Prepare an Agar Petri dish using the right procedures  Identify the materials used in the preparation
  3. 3. Introduction: Agar is a gelatinous substance perfect for biological experiments since it holds up bacteria and does not disintegrate quickly. You can use a bottle of specially prepared liquid, tablet or powder to make your Agar plate.
  4. 4. Procedures:  1 Begin by sterilizing the Petri dishes. Place in a bath of boiling water and allow them to dry upside down on a sanitized drying rack or lab work bench. If the Petri dishes are still enclosed in the original packaging, leave them until ready to use. For 500 milliliters of agar mixture, you can fill 25 Petri dishes.
  5. 5. Procedures:  2 Prepare agar powder by placing it in the microwave with the appropriate amount of water. The label should provide specific directions although 6.9 grams to 500 ml of water is standard. For agar tablets, 10 tablets dissolved in two cups of water is standard.
  6. 6. Procedures:  3 Treat bottled agar formula carefully by loosening the bottle cap (but not removing) before placing in the microwave to warm and soften into a liquid state. Sterilize the bottle after microwaving by placing the neck of the bottle over an open flame a few times to ensure any airborne germs are eradicated before proceeding to plate making.
  7. 7. Procedures:  4 Place the Petri dishes the right way up and crack but do not remove the lids. This is to prepare them for individual handling when the other hand is holding the agar mixture in a suitable pouring vessel such as a glass pitcher.  5 Gently lift the lid of the Petri dish with one hand and hover the lid immediately over the bottom dish to remove any chance of airborne germs entering.
  8. 8. Procedures:  6 Pour an amount of agar liquid into each dish as you hover the lid. About 1/8 inch is the standard thickness of the agar liquid.  7 Allow the agar to set into the dishes by leaving them on a flat, clean surface to dry in room temperature. Secure lids once set and store upside down.

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