EVALUATION OF CITREX® ACTIVITY VS . A CHLORINE SOLUTION AS A DISINFECTANT DURING RAW SHRIMP PACKING PROCESS
PROCEDURE <ul><li>Samples were collected using the following protocol: </li></ul><ul><li>Product must be prepared using potable water, in agreement with the recommended dose rate: </li></ul><ul><li>2 milliliters per liter of solution for a 400 ppm final concentration. Concentrated product solution was 200,000 ppm (20%). </li></ul><ul><li>A fresh working solution must be prepared on the day of use, even though the molecule is stable, and preparation on the previous day does not decrease product’s microbicidal potency. </li></ul><ul><li>1.- Technical shrimp processing steps were performed. </li></ul><ul><li>2.- A 400 ppm Lonlife solution is used for final rinsing. This solution is not rinsed, but allowed to act on shrimp. </li></ul><ul><li>3.- Proceed with packing and freezing processes. </li></ul>
<ul><li>These assays were performed using a 200 ppm dyed Lonlife, solution, consistent with company’s regular practices, and without the previous use of any disinfectants. </li></ul><ul><li>Assays were carried out on both white shrimp (sea catch) and farm-harvested shrimp . </li></ul><ul><li>Assays were performed during different working shifts. A total of 3 assays were performed per product (i.e. 3 assays for sea shrimp and 3 for farm shrimp). </li></ul>
DETERMINATION OF PRODUCT-ESCORT HETEROTROPHIC BIOMASS <ul><li>Total mesophyllic microflora count was determined using PCA agar and deep seeding. </li></ul><ul><li>The first 1:100 dilution was obtained as follows: A 10-g shrimp sample was macerated using a sterilized mortar then diluted in 90 ml sterile 1% peptone water. </li></ul><ul><li>Afterwards, 1:100 serial dilutions were prepared by adding 1 ml of the first solution to the next, until a 10 5 dilution was obtained . </li></ul>
<ul><li>Preparation was then seeded by pipetting 1 ml of the mix to the bottom of a Petri dish. 20 ml PCA agar were added then incubated for 24 - 48 hours at 35°C. After this time results were read. </li></ul><ul><li>During January 2006 the first 12 samples were received. All 12 samples were processed in duplicate. </li></ul>
COLIMETRIC DETERMINATION TO ASSESS PRODUCT SANITARY SATUS POSTDISINFECTION <ul><li>Coliforms and E. coli were quantified by colimetric analysis. For this purpose the most probable number (MPN) technique was applied, using Brilla medium. </li></ul>
VIBRIO GENUS RESEARCH <ul><li>The genus V ibrio was studied on the samples submitted, using the 10 1 dilution exhaustion technique. Positive samples and isolated colonies were then biochemically identified. </li></ul>
CONCLUSIONS <ul><li>Microbiological studies and further result comparison showed that chlorine solutions had a lower performance (i.e.: they resulted in lower significant decreases of the organisms analyzed). </li></ul><ul><li>More research is needed to further support the disinfectant effect of organic acid complexes like Citrex®. </li></ul>
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