Citrex610 f-en-citrex vs cloro en camaron
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Citrex610 f-en-citrex vs cloro en camaron

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  • 1. EVALUATION OF CITREX® ACTIVITY VS . A CHLORINE SOLUTION AS A DISINFECTANT DURING RAW SHRIMP PACKING PROCESS
  • 2. PROCEDURE
    • Samples were collected using the following protocol:
    • Product must be prepared using potable water, in agreement with the recommended dose rate:
    • 2 milliliters per liter of solution for a 400 ppm final concentration. Concentrated product solution was 200,000 ppm (20%).
    • A fresh working solution must be prepared on the day of use, even though the molecule is stable, and preparation on the previous day does not decrease product’s microbicidal potency.
    • 1.- Technical shrimp processing steps were performed.
    • 2.- A 400 ppm Lonlife solution is used for final rinsing. This solution is not rinsed, but allowed to act on shrimp.
    • 3.- Proceed with packing and freezing processes.
  • 3.
    • These assays were performed using a 200 ppm dyed Lonlife, solution, consistent with company’s regular practices, and without the previous use of any disinfectants.
    • Assays were carried out on both white shrimp (sea catch) and farm-harvested shrimp .
    • Assays were performed during different working shifts. A total of 3 assays were performed per product (i.e. 3 assays for sea shrimp and 3 for farm shrimp).
  • 4. DETERMINATION OF PRODUCT-ESCORT HETEROTROPHIC BIOMASS
    • Total mesophyllic microflora count was determined using PCA agar and deep seeding.
    • The first 1:100 dilution was obtained as follows: A 10-g shrimp sample was macerated using a sterilized mortar then diluted in 90 ml sterile 1% peptone water.
    • Afterwards, 1:100 serial dilutions were prepared by adding 1 ml of the first solution to the next, until a 10 5 dilution was obtained .
  • 5.
    • Preparation was then seeded by pipetting 1 ml of the mix to the bottom of a Petri dish. 20 ml PCA agar were added then incubated for 24 - 48 hours at 35°C. After this time results were read.
    • During January 2006 the first 12 samples were received. All 12 samples were processed in duplicate.
  • 6.  
  • 7. 400 ppm Citrex Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 3,000 Sample 1 400 Sample 2 4,000 Sample 2 4,000 Sample 3 1,800 Sample 3 3,000
  • 8. 200 ppm Chlorine Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 7,800 Sample 1 11,000 Sample 2 12,000 Sample 2 4,500 Sample 3 16,000 Sample 3 7,000
  • 9. Farm shrimp 400 ppm Citrex Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 520 Sample 1 280 Sample 2 310 Sample 2 1,500 Sample 3 5,400 Sample 3 1,100 Sample 4 2,700 Sample 4 950
  • 10. 200 ppm Chlorine Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 3,500 Sample 1 1,500 Sample 2 4,700 Sample 2 2,600 Sample 3 1,900 Sample 3 2,200 Sample 4 3,300 Sample 4 1,100
  • 11. COLIMETRIC DETERMINATION TO ASSESS PRODUCT SANITARY SATUS POSTDISINFECTION
    • Coliforms and E. coli were quantified by colimetric analysis. For this purpose the most probable number (MPN) technique was applied, using Brilla medium.
  • 12.  
  • 13.  
  • 14.  
  • 15. Coliforms - 400 ppm Citrex Assay 1 Assay 2 Sample Coliforms E. coli Sample Coliforms E. coli Sample 1 90 4 Sample 1 90 4 Sample 2 23 4 Sample 2 23 4 Sample 3 4 < 3 Sample 3 4 < 3
  • 16. 200 ppm Chlorine Assay 1 Assay 2 Sample Coliforms E. coli Sample Sample E. coli Sample 1 200 21 Sample 1 200 9 Sample 2 200 3 Sample 2 200 3 Sample 3 200 40 Sample 3 200 9
  • 17. Coliform Assays: Only farm shrimp, 400 ppm Citrex (March) Assay 1 Assay 2 Sample Coliforms E. coli Sample Coliforms E. coli Sample 1 11 < 3 Sample 1 9 < 3 Sample 2 7 <3 Sample 2 4 <3 Sample 3 3 < 3 Sample 3 11 3 Sample 4 21 3 Sample 4 9 3
  • 18. Farm Shrimp - 200 ppm Chlorine Assay 1 Assay 2 Sample Coliforms E. coli Sample Coliforms E. coli Sample 1 11 <3 Sample 1 9 4 Sample 2 70 7 Sample 2 15 3 Sample 3 9 3 Sample 3 <3 <3 Sample 4 21 <3 Sample 4 23 11
  • 19. GENUS DETERMINATION Salmonella spp.
  • 20.  
  • 21.  
  • 22.  
  • 23. Salmonella spp. Research 400 ppm Citrex Assay 1 Assay 2 Sample Salmonella spp. Sample Salmonella spp. Sample 1 Negative Sample 1 Negative Sample 2 Negative Sample 2 Negative Sample 3 Negative Sample 3 Negative
  • 24. Sea shrimp - Salmonella 200 ppm chlorine Assay 1 Assay 2 ID Sample Salmonella spp. Sample Salmonella spp. Sample 1 POSITIVE Sample 1 POSITIVE Salmonella enteritidis Sample 2 POSITIVE Sample 2 POSITIVE Salmonella enteritidis Sample 3 Negative Sample 3 Negative
  • 25. VIBRIO GENUS RESEARCH
    • The genus V ibrio was studied on the samples submitted, using the 10 1 dilution exhaustion technique. Positive samples and isolated colonies were then biochemically identified.
  • 26. Sea shrimp – Vibrio: 400 ppm Citrex Assay 1 Assay 2 Sample Vibrio spp. Sample Vibrio spp. Sample 1 Negative Sample 1 Negative Sample 2 Negative Sample 2 Negative Sample 3 Negative Sample 3 Negative
  • 27. Sea shrimp - Vibrio 200 ppm Chlorine Assay 1 Assay 2 Sample Vibrio spp. Sample Vibrio spp. Sample 1 Negative Sample 1 Negative Sample 2 Negative Sample 2 POSITIVE Sample 3 Negative Sample 3 Negative
  • 28.  
  • 29.  
  • 30. Mesophylls Decrease – January Samples No. of samplings Chlorine
  • 31. Mesophylls Decrease – March Samples No. of samplings Chlorine
  • 32. Coliform Decrease – January No. of samplings Chlorine
  • 33. E. coli Decrease No. of samplings Chlorine
  • 34. CONCLUSIONS
    • Microbiological studies and further result comparison showed that chlorine solutions had a lower performance (i.e.: they resulted in lower significant decreases of the organisms analyzed).
    • More research is needed to further support the disinfectant effect of organic acid complexes like Citrex®.