EVALUATION OF CITREX® ACTIVITY  VS . A  CHLORINE SOLUTION AS A DISINFECTANT DURING RAW SHRIMP PACKING PROCESS
PROCEDURE   <ul><li>Samples were collected using the following protocol: </li></ul><ul><li>Product must be prepared using ...
<ul><li>These assays were performed using a 200 ppm dyed Lonlife, solution, consistent with company’s regular practices, a...
DETERMINATION OF PRODUCT-ESCORT HETEROTROPHIC  BIOMASS <ul><li>Total mesophyllic microflora count was determined using PCA...
<ul><li>Preparation was then seeded by pipetting 1 ml of the mix to the bottom of a Petri dish. 20 ml PCA agar were added ...
 
400 ppm Citrex Assay 1 Assay 2 Sample  Mesophylls Sample  Mesophylls  Sample 1 3,000 Sample 1 400 Sample 2 4,000 Sample 2 ...
200 ppm Chlorine Assay 1 Assay 2 Sample  Mesophylls Sample  Mesophylls Sample 1 7,800 Sample 1 11,000 Sample 2 12,000 Samp...
Farm shrimp 400 ppm Citrex Assay 1 Assay 2 Sample  Mesophylls  Sample  Mesophylls  Sample 1 520 Sample 1 280 Sample 2 310 ...
200 ppm Chlorine Assay 1 Assay 2 Sample  Mesophylls Sample  Mesophylls Sample 1 3,500 Sample 1 1,500 Sample 2 4,700 Sample...
COLIMETRIC DETERMINATION TO ASSESS PRODUCT  SANITARY SATUS POSTDISINFECTION <ul><li>Coliforms and  E. coli  were quantifie...
 
 
 
Coliforms - 400 ppm Citrex Assay 1 Assay 2 Sample  Coliforms E. coli Sample Coliforms E. coli Sample 1 90 4 Sample 1 90 4 ...
200 ppm Chlorine Assay 1 Assay 2 Sample  Coliforms E. coli Sample  Sample E. coli Sample 1 200 21 Sample 1 200 9 Sample 2 ...
Coliform Assays: Only farm shrimp, 400 ppm Citrex (March) Assay 1 Assay 2 Sample  Coliforms  E. coli Sample  Coliforms  E....
Farm Shrimp - 200 ppm Chlorine Assay 1 Assay 2 Sample Coliforms E. coli Sample Coliforms E. coli Sample 1 11 <3 Sample 1 9...
GENUS DETERMINATION Salmonella  spp.
 
 
 
Salmonella  spp. Research 400 ppm Citrex Assay 1 Assay 2 Sample  Salmonella  spp. Sample Salmonella  spp. Sample 1 Negativ...
Sea shrimp -  Salmonella   200 ppm chlorine Assay 1 Assay 2 ID Sample  Salmonella  spp. Sample Salmonella  spp. Sample 1 P...
VIBRIO GENUS RESEARCH <ul><li>The genus V ibrio  was studied on the samples submitted, using the 10 1  dilution exhaustion...
Sea shrimp – Vibrio: 400 ppm Citrex Assay 1 Assay 2 Sample  Vibrio  spp. Sample Vibrio  spp. Sample 1 Negative Sample 1 Ne...
Sea shrimp - Vibrio  200 ppm Chlorine Assay 1 Assay 2 Sample Vibrio  spp. Sample Vibrio  spp. Sample 1 Negative Sample 1 N...
 
 
Mesophylls Decrease – January Samples No. of samplings Chlorine
Mesophylls Decrease – March Samples No. of samplings Chlorine
Coliform Decrease – January No. of samplings Chlorine
E. coli  Decrease No. of samplings Chlorine
CONCLUSIONS   <ul><li>Microbiological studies and further result comparison showed that chlorine solutions had a lower per...
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Citrex610 f-en-citrex vs cloro en camaron

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Citrex610 f-en-citrex vs cloro en camaron

  1. 1. EVALUATION OF CITREX® ACTIVITY VS . A CHLORINE SOLUTION AS A DISINFECTANT DURING RAW SHRIMP PACKING PROCESS
  2. 2. PROCEDURE <ul><li>Samples were collected using the following protocol: </li></ul><ul><li>Product must be prepared using potable water, in agreement with the recommended dose rate: </li></ul><ul><li>2 milliliters per liter of solution for a 400 ppm final concentration. Concentrated product solution was 200,000 ppm (20%). </li></ul><ul><li>A fresh working solution must be prepared on the day of use, even though the molecule is stable, and preparation on the previous day does not decrease product’s microbicidal potency. </li></ul><ul><li>1.- Technical shrimp processing steps were performed. </li></ul><ul><li>2.- A 400 ppm Lonlife solution is used for final rinsing. This solution is not rinsed, but allowed to act on shrimp. </li></ul><ul><li>3.- Proceed with packing and freezing processes. </li></ul>
  3. 3. <ul><li>These assays were performed using a 200 ppm dyed Lonlife, solution, consistent with company’s regular practices, and without the previous use of any disinfectants. </li></ul><ul><li>Assays were carried out on both white shrimp (sea catch) and farm-harvested shrimp . </li></ul><ul><li>Assays were performed during different working shifts. A total of 3 assays were performed per product (i.e. 3 assays for sea shrimp and 3 for farm shrimp). </li></ul>
  4. 4. DETERMINATION OF PRODUCT-ESCORT HETEROTROPHIC BIOMASS <ul><li>Total mesophyllic microflora count was determined using PCA agar and deep seeding. </li></ul><ul><li>The first 1:100 dilution was obtained as follows: A 10-g shrimp sample was macerated using a sterilized mortar then diluted in 90 ml sterile 1% peptone water. </li></ul><ul><li>Afterwards, 1:100 serial dilutions were prepared by adding 1 ml of the first solution to the next, until a 10 5 dilution was obtained . </li></ul>
  5. 5. <ul><li>Preparation was then seeded by pipetting 1 ml of the mix to the bottom of a Petri dish. 20 ml PCA agar were added then incubated for 24 - 48 hours at 35°C. After this time results were read. </li></ul><ul><li>During January 2006 the first 12 samples were received. All 12 samples were processed in duplicate. </li></ul>
  6. 7. 400 ppm Citrex Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 3,000 Sample 1 400 Sample 2 4,000 Sample 2 4,000 Sample 3 1,800 Sample 3 3,000
  7. 8. 200 ppm Chlorine Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 7,800 Sample 1 11,000 Sample 2 12,000 Sample 2 4,500 Sample 3 16,000 Sample 3 7,000
  8. 9. Farm shrimp 400 ppm Citrex Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 520 Sample 1 280 Sample 2 310 Sample 2 1,500 Sample 3 5,400 Sample 3 1,100 Sample 4 2,700 Sample 4 950
  9. 10. 200 ppm Chlorine Assay 1 Assay 2 Sample Mesophylls Sample Mesophylls Sample 1 3,500 Sample 1 1,500 Sample 2 4,700 Sample 2 2,600 Sample 3 1,900 Sample 3 2,200 Sample 4 3,300 Sample 4 1,100
  10. 11. COLIMETRIC DETERMINATION TO ASSESS PRODUCT SANITARY SATUS POSTDISINFECTION <ul><li>Coliforms and E. coli were quantified by colimetric analysis. For this purpose the most probable number (MPN) technique was applied, using Brilla medium. </li></ul>
  11. 15. Coliforms - 400 ppm Citrex Assay 1 Assay 2 Sample Coliforms E. coli Sample Coliforms E. coli Sample 1 90 4 Sample 1 90 4 Sample 2 23 4 Sample 2 23 4 Sample 3 4 < 3 Sample 3 4 < 3
  12. 16. 200 ppm Chlorine Assay 1 Assay 2 Sample Coliforms E. coli Sample Sample E. coli Sample 1 200 21 Sample 1 200 9 Sample 2 200 3 Sample 2 200 3 Sample 3 200 40 Sample 3 200 9
  13. 17. Coliform Assays: Only farm shrimp, 400 ppm Citrex (March) Assay 1 Assay 2 Sample Coliforms E. coli Sample Coliforms E. coli Sample 1 11 < 3 Sample 1 9 < 3 Sample 2 7 <3 Sample 2 4 <3 Sample 3 3 < 3 Sample 3 11 3 Sample 4 21 3 Sample 4 9 3
  14. 18. Farm Shrimp - 200 ppm Chlorine Assay 1 Assay 2 Sample Coliforms E. coli Sample Coliforms E. coli Sample 1 11 <3 Sample 1 9 4 Sample 2 70 7 Sample 2 15 3 Sample 3 9 3 Sample 3 <3 <3 Sample 4 21 <3 Sample 4 23 11
  15. 19. GENUS DETERMINATION Salmonella spp.
  16. 23. Salmonella spp. Research 400 ppm Citrex Assay 1 Assay 2 Sample Salmonella spp. Sample Salmonella spp. Sample 1 Negative Sample 1 Negative Sample 2 Negative Sample 2 Negative Sample 3 Negative Sample 3 Negative
  17. 24. Sea shrimp - Salmonella 200 ppm chlorine Assay 1 Assay 2 ID Sample Salmonella spp. Sample Salmonella spp. Sample 1 POSITIVE Sample 1 POSITIVE Salmonella enteritidis Sample 2 POSITIVE Sample 2 POSITIVE Salmonella enteritidis Sample 3 Negative Sample 3 Negative
  18. 25. VIBRIO GENUS RESEARCH <ul><li>The genus V ibrio was studied on the samples submitted, using the 10 1 dilution exhaustion technique. Positive samples and isolated colonies were then biochemically identified. </li></ul>
  19. 26. Sea shrimp – Vibrio: 400 ppm Citrex Assay 1 Assay 2 Sample Vibrio spp. Sample Vibrio spp. Sample 1 Negative Sample 1 Negative Sample 2 Negative Sample 2 Negative Sample 3 Negative Sample 3 Negative
  20. 27. Sea shrimp - Vibrio 200 ppm Chlorine Assay 1 Assay 2 Sample Vibrio spp. Sample Vibrio spp. Sample 1 Negative Sample 1 Negative Sample 2 Negative Sample 2 POSITIVE Sample 3 Negative Sample 3 Negative
  21. 30. Mesophylls Decrease – January Samples No. of samplings Chlorine
  22. 31. Mesophylls Decrease – March Samples No. of samplings Chlorine
  23. 32. Coliform Decrease – January No. of samplings Chlorine
  24. 33. E. coli Decrease No. of samplings Chlorine
  25. 34. CONCLUSIONS <ul><li>Microbiological studies and further result comparison showed that chlorine solutions had a lower performance (i.e.: they resulted in lower significant decreases of the organisms analyzed). </li></ul><ul><li>More research is needed to further support the disinfectant effect of organic acid complexes like Citrex®. </li></ul>
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